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Citations for Miltenyi Biotec :
1801 - 1850 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... freshly isolated neutrophils were resuspended in 100μL MACS Separation Buffer (130-091-221, Miltenyi Biotec Inc.) and stained with APC-conjugated anti-human CSTA antibody (2μL/106 cells ...
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bioRxiv - Immunology 2024Quote: ... cells labeled with MACS beads are captured by the magnetic field of the separator (130-090-312, Miltenyi Biotec Inc.), whereas unlabeled cells pass the magnetic field and end up in the flow through fraction ...
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bioRxiv - Immunology 2024Quote: ... freshly isolated neutrophils were resuspended in 100μL MACS Separation Buffer (130-091-221, Miltenyi Biotec Inc.) and stained with PE-conjugated anti-human CCL4 antibody (2 μL/106 cells ...
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bioRxiv - Immunology 2024Quote: ... cells labeled with MACS beads are captured by the magnetic field of the separator (130-090-312, Miltenyi Biotec Inc), whereas unlabeled cells pass the magnetic field and end up in the flow through fraction ...
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bioRxiv - Immunology 2024Quote: ... in 80μL MACS Separation Buffer (130-091-221, Miltenyi Biotec Inc.) for 15min in the dark ...
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bioRxiv - Immunology 2024Quote: ... PDC were isolated via autoMACS or MidiMACS using LS Columns (Miltenyi Biotec, Bergisch-Gladbach, Germany), with the anti-CD304 (BDCA-4/Neuropilin-1 ...
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bioRxiv - Immunology 2024Quote: PDC were depleted from PBMC by AutoMACS (deplete_s) after preincubation with Fc block and anti-CD304 microbeads (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). Total PBMC controls were subject to the same AUTOMACS protocol ...
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bioRxiv - Immunology 2024Quote: ... with the anti-CD304 (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) and identified as BDCA-2 positive ...
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bioRxiv - Immunology 2024Quote: ... Cells were then passed through LD separation columns (Miltenyi Biotec) placed on magnetic stands ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, cat. no. 130-090-101). Live cells were diluted to 1000 cells/µL with ice-cold 0.04% BSA in PBS and sent to Genome Access Technology Center at the McDonnell Genome Institute for library preparation using 10x Genomics Chromium Single Cell 5’ Reagent Kits and sequencing ...
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bioRxiv - Immunology 2024Quote: ... separation and MACS-sorting according to protocol (Miltenyi Biotec). After OKT3-activation (plate-bound ...
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bioRxiv - Immunology 2024Quote: ... T cells from Buffy coats of healthy donors were isolated by Ficoll separation followed by CD8- and CD4-magnetic bead isolation according to protocol (Miltenyi Biotec). After activation with plate-bound OKT-3 (30ng/μl ...
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bioRxiv - Immunology 2024Quote: ... and were cultured with αCD40 (150 ng/mL; clone FGK-45; Miltenyi Biotec) and IL-4 (25 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 80uL buffer and 20uL CD45 microbeads (Miltenyi Biotec 130-109-682) at 4°C for 15 minutes before adding to a prepared Pre-Separation Filter (130-101-812 ...
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bioRxiv - Immunology 2024Quote: ... The CD11c+ DCs were isolated from the PP-derived cells using CD11c Micro Beads Ultra Pure (Miltenyi Biotec). The total RNA was isolated from the DCs using TRIzolTM reagent (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... mouse were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2024Quote: ... the cells were harvested and CD11c+ population was enriched using CD11c Micro Beads UltraPure (Miltenyi Biotec, Bergisch Gladbach, Germany). The quality of BMDCs was analyzed by flow cytometry in every culture ...
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bioRxiv - Immunology 2024Quote: ... Fc-receptor blocking was performed following manufacturer’s instructions (Miltenyi Biotec). Cells were washed twice with PBS and centrifuged (800 g ...
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bioRxiv - Immunology 2024Quote: ... Imaging was performed using a LaVision Ultramicroscope II (Miltenyi Biotec). Image stacks were acquired with a step size of 6 µm at x2 magnification using an optic zoom with a numerical aperture of 9 µm ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in Red Blood Cell Lysis Solution (Miltenyi Biotec) for 5 min at room temperature and then passed through a 40μm strainer ...
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bioRxiv - Immunology 2024Quote: ... Cells were then stained with CD45 and CD31 MicroBeads (Miltenyi Biotec) for magnetic depletion of immune and endothelial cells according to manufacturer’s protocol and then stained with a fluorescent-conjugated EpCAM antibody for FACS sorting(6 ...
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bioRxiv - Immunology 2024Quote: ... collected with BD FACSDiva 6.3.1 software and analyzed using Flowlogic software (Miltenyi Biotec). All antibodies used for extracellular and intracellular stainings are listed in the supplemental table 2.
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bioRxiv - Immunology 2024Quote: Tumors were mechanically dissociated with scissors and digested enzymatically using the Tumor Dissociation Kit mouse and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... After filtration through a 70 μm cell strainer (Miltenyi Biotec), the single-cell suspension was washed two times with complete RPMI-1640 ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+CD44- T lymphocytes were subsequently isolated using the CD8α T Cell Isolation Kit (Miltenyi biotec) through addition of an anti-CD44 biotinylated antibody (clone IM7 ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured at 37°C ...
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bioRxiv - Immunology 2024Quote: ... we enriched CD8+ T cells using a mouse CD8α T Cell Isolation kit (Miltenyi Biotec) to >95% purity as verified by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... securely placed in a MACSmix tube rotator (Miltenyi Biotec) in an incubator at 37°C with 5%CO2 ...
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bioRxiv - Immunology 2024Quote: ... magnetic-activated cell sorting (MACS) was performed using Anti-Ly6G Microbeads Ultrapure (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Mouse whole blood samples were processed as follows: 200 µL of blood was incubated with 2ml of 1x Red Blood Cell Lysis buffer diluted in water (Miltenyi Biotec #130-094-183) for 10min at RT ...
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bioRxiv - Immunology 2024Quote: ... and tumors were harvested and digested with Tumor Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec). T cells were isolated from target K562 cells using the Dynabeads™ Untouched™ Human T Cells Kit (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... mouse and AutoMACS® Pro Separator (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Antibody-labeled cells were depleted by labeling with anti-FITC MACS beads (Miltenyi Biotec, catalog# 130-048-701) and subsequent negative sorting using autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... and subsequent negative sorting using autoMACS Pro Separator (Miltenyi Biotec). The enriched CD4+ T cell fraction was further stained with PerCP-Cy5.5-labeled anti-CD4 mAb and APC-labeled anti-CD62L mAb ...
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bioRxiv - Immunology 2024Quote: ... and GentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The lungs were processed with Lung Dissociation Kit (Miltenyi Biotec, catalog# 130-095-927) and GentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The lymphoid organs were then ground through a 30 μm filter mesh (Miltenyi Biotec, Bergisch Gladbach, Germany) and washed with HBSS/BSA (Sigma Life Science ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were fully homogenized using RNA_01.01 program provided in gentleMACS™ Dissociator (Miltenyi Biotec, Germany). The lysate was then incubated with proteinase K (600 mAU/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... the homogenate was incubated with microbeads conjugated to a cocktail of monoclonal antibodies against no-target cells (Miltenyi Biotec, Bergisch Gladbach, Germany) at 2-8 °C for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell suspension was applied onto the MACS Column placed in a MACS Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). We first collected the flow-through containing unlabeled cells ...
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bioRxiv - Bioengineering 2024Quote: ... 1% penicillin-streptomycin and 5ng/ml Human Recombinant Fibroblast Growth factor-2 (FGF-2) (130093564, Miltenyi Biotec). Fibroblasts were passaged using trypsin EDTA 0.5% ...
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bioRxiv - Bioengineering 2024Quote: ... lineage cells were depleted with a lineage cell depletion kit (#130-090-858, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 20ng/ml ACTIVIN A (Miltenyi Biotec, #130-115-009) and 1.75μM CHIR99021 (Selleckchem ...
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bioRxiv - Bioengineering 2024Quote: ... Mouse cells were removed using the Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694). Dissociated cells from two mice per condition were pooled together ...
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bioRxiv - Bioengineering 2024Quote: ... Myelin was removed using Myelin Removal Beads II (Miltenyi Biotec, 130-096-731). Mouse cells were removed using the Mouse Cell Depletion Kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... Spleens were dissociated in gentleMACS C Tubes (Miltenyi Biotec, 130-093-237), filtered through a 70 µm mesh and treated with ammonium-chloride-potassium lysis buffer to eliminate red blood cells ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...