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Citations for Miltenyi Biotec :
1701 - 1750 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells underwent a TCRαβ depletion step (anti-TCRαβ antibody REA652 clone, anti-biotin microbeads and LD column, all Miltenyi Biotec) with an overnight recovery followed by cryopreservation in 45% TexMACS media (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... activated with TransAct reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured at a density of 1×106 per mL in TexMACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... with an overnight recovery followed by cryopreservation in 45% TexMACS media (Miltenyi Biotec), 45% heat-inactivated human serum (Seralab) ...
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bioRxiv - Immunology 2024Quote: ... media with 3% heat-inactivated human serum (Seralab, Sussex, UK) and 20 ng/mL human recombinant IL-2 (Miltenyi Biotec). BE-CAR7 products and CART19 control cells were made as previously described using pCCL-CAR7 or pTT-CAR19 vector ...
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bioRxiv - Immunology 2024Quote: ... and cultured at a density of 1×106 per mL in TexMACS (Miltenyi Biotec) media with 3% heat-inactivated human serum (Seralab ...
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bioRxiv - Immunology 2024Quote: ... Mouse and Rat (Miltenyi Biotec, Gaithersburg, MD; Cat No. 130-107-677) per manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... and subjected them to anti-mouse CD45 bead-based separation (Miltenyi Biotec; Cat. No 130-052-301) per manufactural protocol ...
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bioRxiv - Immunology 2024Quote: ... mouse (Miltenyi Biotec). Total RNA was isolated from the DCs using TRIzol (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and put into gentleMACS C-tubes (Miltenyi Biotec, #130-093-237) with dissociation enzymes prepared for initial tissue homogenization using the 37C_ABDK_01 protocol on the gentleMACS Octo-Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... with dissociation enzymes prepared for initial tissue homogenization using the 37C_ABDK_01 protocol on the gentleMACS Octo-Dissociator with heaters (Miltenyi Biotec, #130-096-427) for dissociation ...
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bioRxiv - Immunology 2024Quote: ... BRD1-KO cells were re-stimulated with anti-CD3 and anti-CD28 T cell activation beads (TAB, Miltenyi Biotec) and cytokines (IL-1β and IL-23 ...
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bioRxiv - Immunology 2024Quote: ... Memory CD4+ T cells were isolated using an untouched negative isolation kit (Miltenyi Biotec). Isolated memory CD4+ T cells were stimulated with plate bound α-CD3 (5 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... Treg cells were then isolated from PBMCs using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Primary human monocytes were positively enriched from buffy coat PBMCs with CD14 Microbeads and LS columns (Miltenyi Biotec). TrueCut Cas9 Protein v2 and TrueGuide Synthetic single guide (sg)RNAs (both from Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... on an autoMACS Pro Separator (Miltenyi Biotec). Naïve T cells (106 cells/ml ...
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bioRxiv - Immunology 2024Quote: ... and passed through LS columns attached to MidiMACS or QuadroMACS separators (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... incubated with streptavidin microbeads (Miltenyi Biotec) and passed through LS columns attached to MidiMACS or QuadroMACS separators (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and positively selected using LS columns (Miltenyi Biotec). Enriched CD64+ cells were stained with antibodies (Table S1 ...
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bioRxiv - Genetics 2024Quote: ... Cells were washed three times in permeabilization buffer and re-suspended in autoMACS Running Buffer (130-091-221, Miltenyi Biotec, Bergisch Gladbach, Germany). We used several negative controls in each flow cytometry experiment ...
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bioRxiv - Immunology 2024Quote: ... Cells were simultaneously stained with CD45 microbeads (Miltenyi Biotec) and antibodies ...
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bioRxiv - Immunology 2024Quote: ... Hematopoietic cells were depleted using the LS MACS (Miltenyi Biotec) column as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Isolated T cells were stimulated with plate-bound anti-CD3 and anti-CD28 (Miltenyi Biotec) at 2 million cells/ml ...
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bioRxiv - Immunology 2024Quote: Primary T cells were sorted from primary PBMC from healthy donors using Pan T cell isolation kit – human (Miltenyi Biotec, # 130-096-535). Isolated T cells were stimulated with plate-bound anti-CD3 and anti-CD28 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and anti-biotin MicroBeads (Miltenyi Biotec, 15min) with washes in MACS buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... followed by purification with magnetic anti-CD14 microbeads (Miltenyi Biotec, LS Columns 130-042-401; USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... lungs were digested to single cell suspension using the gentleMACS lung dissociation kit and dissociator per the manufacturer’s instructions (Miltenyi Biotec). BALF cells or lung digest cells were stained with Ly6G-APC-Cy7 (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were then enriched using the Anti-PE MicroBeads kit (Miltenyi Biotec). Cells were subsequently surface stained for FACS sorting ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ Tregs and CD4+CD25− T cells were purified from the peripheral LNs and spleen of Apoe−/− or Ccr4−/−Apoe−/− mice using a CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) and anti-CD4 beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The production of several major cytokines from CD4+ T cells was examined as described previously.30 Splenic CD4+ T cells (1×105 cells) isolated using MACS (Miltenyi Biotec) were stimulated with plate-bound anti-CD3 (10 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD11c+ DCs were isolated from spleen of Apoe−/− mice treated with Liberase TM using MACS (Miltenyi Biotec). The purity of the CD11c+ population was approximately 93% ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD4 beads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... via immunomagnetic isolation (Miltenyi Biotec). Extended experimental details are reported in the supplementary methods.
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bioRxiv - Immunology 2024Quote: ... 1 µg/ml PepTivator SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec), or an equivalent volume of DMSO (or sterile water as indicated ...
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bioRxiv - Immunology 2024Quote: ... and anti-PE microbeads (Miltenyi Biotec) as per manufacturer’s protocol were seeded at 1-2e6 cells per well ...
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bioRxiv - Immunology 2024Quote: ... human (Miltenyi Biotec). Cells were frozen and kept in nitrogen until DNA extraction and sequencing as stated below.
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bioRxiv - Immunology 2024Quote: ... Neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions within 1–2 hours of blood draw ...
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bioRxiv - Immunology 2024Quote: ... erythrocyte depletion (Miltenyi Biotec) was performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells (tNGFR+) were sorted using MACSelect LNGFR microbeads (Miltenyi Biotec, 130-091-330).
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated using CD14 microbeads (Miltenyi Biotec, 130-050-201) and plated at 150’000 cells/cm2 in F+ high attachment plates (Sarstedt ...
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bioRxiv - Immunology 2024Quote: ... and (iii) anti-NKG2A-VioBright FITC (clone REA110) from Miltenyi Biotec. Cytosolic/intranuclear expression of Ki67 was detected using anti-Ki67–BV421 (clone B56 ...
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bioRxiv - Immunology 2024Quote: ... F4/80+ macrophages were positively selected using Microbeads (Catalog# 130-110-443, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... or MACS anti-REA Comp Bead kit (Miltenyi Biotec). Data were acquired using an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Naive B cells were isolated from the peripheral lymph nodes of mice using the B cell isolation kit (Miltenyi Biotec) and cultured in Dutch-modified RPMI-1640 media supplemented with 8% FBS ...
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bioRxiv - Immunology 2024Quote: ... Brains were immediately removed and dissociated using a Neural Tissue Dissociation kit (P) (Miltenyi Biotec, #130-092-628). Cells were then resuspended in 30% Percoll solution diluted in RPMI medium ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed again and blocked using 1% Fc block (Miltenyi Biotec) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... and 1% Fc block (Miltenyi Biotec) in FACS buffer were added for 15 minutes (4°C) ...
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bioRxiv - Immunology 2024Quote: ... The cell pellet was then resuspended in MACS buffer and separated using magnetic-activated cell sorting columns according to the manufacturer’s instructions (Miltenyi Biotec). Single-cell suspensions were collected and B cell numbers and purity (CD19+ ...
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bioRxiv - Immunology 2024Quote: ... total CD4+ T cells or total CD8a+ T cells were isolated from WT or Aim2−/−mice using a magnetic cellular isolation kit (CD4+, Miltenyi Biotec, 130-104-454 ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells and naïve CD8a+ T cells were enriched using either a naïve CD4 isolation kit (Miltenyi Biotec, 130-104-453) or naïve CD8a isolation kit (Miltenyi Biotec ...