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Citations for Miltenyi Biotec :
1751 - 1800 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single-cell suspensions using Neural Tissue Dissociation kit (P) (Miltenyi Biotec, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The cells were incubated with anti-CD4 (clone L3T4) covalently linked to microbeads (Miltenyi Biotec) used at a ratio of 100 µL beads/108 cells then incubated for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells were resuspended in binding buffer (cat# 130-090-101, Miltenyi Biotec) with dead cell removal beads ...
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bioRxiv - Bioengineering 2022Quote: ... The solution was passed through a 70 µm cell strainer (Miltenyi Biotec, #130-098-462) followed by a 40 µm cell strainer (Corning ...
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bioRxiv - Developmental Biology 2019Quote: ... adult thymic tissue was depleted of T cells using anti-CD45 MACS beads (Miltenyi Biotec); fetal tissue was not T cell depleted ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were purified using magnetic-activated cell sorting (MACS) with Tra1-60 microbeads (Miltenyi Biotec) on LS columns as described by vendor and cultured to 10-20% confluence ...
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bioRxiv - Systems Biology 2019Quote: ... cell suspensions were stained with anti-mouse CD45-PE-Vio770 (Miltenyi Biotec, 130-110-661), CD326-BV421 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... human CAR T cells were sorted for CD34+ (CD34 MicroBead Kit UltraPure, MACS, Miltenyi Biotec) CAR T cells and expanded for up to 15 days in the presence of human IL-2 ...
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bioRxiv - Immunology 2019Quote: ... and CD8+ T-cells purified by positive magnetic bead selection (Miltenyi Biotec, 130-117-044) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were analyzed on a flow cytometer (MACSQuant Analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany), and data were processed with the MACSQuantify software (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from 16 healthy individuals and enriched for CD19+ B cells (Miltenyi Biotec). Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG− ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysate extracts were incubated with the anti-GFP microbeads (Miltenyi Biotec GmbH, Gladbach, Germany) for 1h ...
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bioRxiv - Microbiology 2021Quote: ... Single-cell suspensions were obtained with adult brain dissociation kit (Miltenyi Biotec, 130-107-677). EGFP positive cells were sorted using the GFP channels of the Bio-Rad S3e instrument ...
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bioRxiv - Immunology 2020Quote: ... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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bioRxiv - Immunology 2019Quote: ... PBMC from 8 healthy blood donors were enriched for CD19+ cells by MACS (Miltenyi Biotec) and 100,000 naive B cells (CD20+IgD+IgM+IgG−CD27− ...
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bioRxiv - Cell Biology 2020Quote: ... and brain microglia cells were isolated using CD11b microbeads and MACS sorting columns (Miltenyi Biotec). Single cell suspensions were subjected to scRNA-seq at the National Cancer Institute single cell analysis Core facility ...
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bioRxiv - Immunology 2020Quote: ... Lungs were processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). The following fluorochrome-conjugated antibodies were used for labelling the cells ...
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bioRxiv - Cell Biology 2021Quote: ... 3-5% parasitemia) were purified using magnetic activated cell sorting (VarioMACS™ Separator, Miltenyi Biotec). Importantly ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated in basal differentiation medium supplemented with 20 ng/ml BMP4 (Miltenyi Biotec) and 10ng/ml FGF10 (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were resuspended in 1mL of AutoMACS Running Buffer (Miltenyi Biotec, Cat. 130-091-221) and passed through a 30 um pre-separation filter (Milteni Biotec ...
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bioRxiv - Microbiology 2021Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... B cells were excluded from whole splenocytes by incubating with anti-CD19 beads (Miltenyi Biotec) or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... PBMC cell suspensions were pre-treated with anti-CD19-conjugated microbeads (Miltenyi Biotec, CA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: Splenic total CD4+T cells were isolated by magnetic sorting using commercial kit (Miltenyi Biotec.) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25− Th cells were purified by positive selection with CD4-specific microbeads (Miltenyi Biotec). Memory T-cell subsets were sorted to more than 97% purity as CD4+CD45RA− RO+CD127+CD25− using APC-780-conjugated anti-CD45RA Ab (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and the BM was depleted of T cells by autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany) utilizing CD90.2 microbeads (#130-121-278 ...
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bioRxiv - Immunology 2020Quote: ... The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632) and CX3CR1 (Biolegend #149039 ...
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bioRxiv - Immunology 2021Quote: Lung epithelial cells separated by magnetic bead: the anti-mouse CD45 magnetic bead (Miltenyi Biotec) was used to enrich the epithelial cells in single-cell suspension ...
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bioRxiv - Immunology 2020Quote: ... and single-cell suspensions were prepared according to the manufacturer’s instructions (Miltenyi Biotec GmbH, 2008). Cells were blocked with CD16/CD32 Fc block (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: Glial cells and neurons were isolated according to the adult brain dissociation protocol (Miltenyi biotec) form corpus callosum and/or cortex ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were isolated from PBMNCs using a magnetic micro-bead CD14+ kit (Miltenyi Biotec), LS columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded to a MACSQuant Tyto HS Cartridge (#130-121-549, Miltenyi Biotec) and sorted according to the instrument instructions until 1 x 107 positive events had been collected ...
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bioRxiv - Immunology 2022Quote: ... samples were first positively selected for CD11c+ cells using Mouse UltraPure CD11c Microbeads (Miltenyi Biotec) prior to surface marker staining ...
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bioRxiv - Immunology 2022Quote: ... CD8 or CD4 T cells were purified from human PBMCs using isolation kits (Miltenyi Biotec) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were positively selected with CD4 (L3T4) magnetic microbeads (Miltenyi Biotec, Auburn, CA), pooled and suspended in PBS ...
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bioRxiv - Immunology 2022Quote: ... ECs were purified by magnetic-activated cell sorting (MACS) using anti-CD146 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... single cells were passed through a 70μm MACS SmartStrainer (Miltenyi Biotec, Cat# 130-098-462) that was pre-wet with 3mL of 20% FBS in PBS into a 15mL conical tube on ice ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The cell suspension was first incubated with FcR blocking reagent (Miltenyi Biotec, 130-092-575) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4+ T cells were isolated from PBMCs by positive selection using CD4+ microbeads (Miltenyi Biotec) or FACS sorting and subsequently expanded through a REP.
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bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
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bioRxiv - Immunology 2023Quote: ... Next we applied the Dead Cell Removal Kit (catalog no. 130-090-101; Miltenyi BioTec) to the remaining cell pellet ...
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bioRxiv - Immunology 2023Quote: ... followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells, Miltenyi Biotec) and the iMag (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were purified using a negative-sorting CD4+ isolation kit (Miltenyi Biotec, Inc.). CD4+ T cell isolation was done following the manufacturer’s instructions and were consistently found to be > 90% pure ...
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bioRxiv - Immunology 2022Quote: ... cells were isolated by positive selection using an LS Column and MidiMACS Separator (Miltenyi Biotec). Cells were resuspended in full DMEM and plated onto collagen I-coated glass or polyacrylamide gels in FluoroDish cell culture dishes (10-mm well ...
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bioRxiv - Genomics 2023Quote: ... we passed the solution through a 30μm cell straining filter (Miltenyi Biotec, Bergisch Gladbach, Germany), which captured the scales (>100 μm length ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with MACS buffer and MACS was performed using an AutoMACS (Miltenyi Biotec) running the program possel_s ...