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Citations for Miltenyi Biotec :
1601 - 1650 of 1728 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Lamina propria fractions were prepared by enzymatic digestion and dissociation with the Lamina Propria Dissociation Kit and GentleMacs Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Dissociated cells were collected for use in qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Biotinylated RNA was incubated with constant rotation for 15 minutes at room temperature with μMACs streptavidin beads and was isolated using μMACs columns (μMACs Strepavidin Kit, Miltenyi Biotec 130-074-101) that had been previously equilibrated with wash buffer (100mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2019Quote: ... the live cell fraction was subjected to a similar MACS based isolation by application of mouse MDSC isolation kit (Miltenyi Biotec, 130-094-538). Isolated cells were washed twice with cold MACS buffer and counted with a hemocytometer and diluted in cold PBS with 0.1% BSA and 2 mM EDTA at desired densities for Drop-seq.
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bioRxiv - Developmental Biology 2020Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was obtained by mechanical resuspension followed by filtration through a 40-μm-pore-size cell strainer and dead cel;ls were removed using Dead Cell Removal Kit (Miltenyi Biotec 130-090-101). Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+ ...
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bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was enzymatically and mechanically dissociated into a single-cell suspension using the magnetic activated cell sorting (MACS) Neural Tissue Dissociation Kit (Miltenyi Biotec 130-093-231). Myelin was removed using the MACS Myelin Removal Beads (Miltenyi Biotec 130-096-733) ...
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bioRxiv - Pathology 2021Quote: Single cells from hamstring muscles were isolated using a skeletal muscle dissociation kit (Miltenyi Biotech) and GentleMACS Dissociator tissue homogenizer (Miltenyi Biotec, Macquarie Park, Australia) as previously described20 ...
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bioRxiv - Immunology 2020Quote: ... OT-I CD8+ T cells were then purified by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec: 130-104-075). For cell division or microscopy studies ...
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bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
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bioRxiv - Immunology 2022Quote: ... and used for eosinophil purifications by negative selection on an AutoMACS Pro separator using the Eosinophil Purification Kit (Cat. No. 130-092-010, Miltenyi Biotec, Bergisch Gladbach, Germany). Eosinophil purity was >98% for all samples as determined by counting a minimum of 300 cells on cytospin slides stained with Shandon™ Diff-Kwik™ stains (Cat ...
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bioRxiv - Cancer Biology 2022Quote: B cells were purified by negative selection from single-cell suspensions from spleen using magnetic separation B cell isolation kit (Miltenyi Biotec 130-090-862). Purified cells were cultured for 4 days in RPMI (Corning ...
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bioRxiv - Neuroscience 2021Quote: Mice were perfused with cold PBS and cortical and hippocampal tissues were dissected and dissociated into a single cell suspension using the Adult Brain Dissociation Kit (Miltenyi Biotec 130-107-677), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: The exosomes extracted from SVV-green fluorescence protein (GFP)-infected cells and normal cells were purified using an exosome purification kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and the copy number of SVV was detected ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining cortex from ipsilateral and contralateral sides were dissected and stored in ice-cold Hibernate E (Brain Bits) until being subjected to enzyme digestion to generate single cell suspensions according to the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677). Myelin was removed from the samples using myelin removal beads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole brain samples were removed and prepared into cell suspension using brain tumor dissociation kit (#130-095-942, Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACSTM Dissociator (#130-093-235 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed by Annexin V exposure and PI incorporation using the FITC-Annexin V/PI kit from Miltenyi Biotec (#130-092-052) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... T cells were enriched from spleen homogenate by magnetic-activated cell sorting (MACS) using Miltenyi Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Single cell suspensions were obtained by adapting the protocol described in (Cowan et al., 2020) and using a papain-based dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
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bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions from liver and BM were depleted of lineage-positive (Lin+) cells using a Direct Lineage Cell Depletion Kit (Miltenyi Biotec, 130-110-470) and stained for the following HSPCs ...
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bioRxiv - Immunology 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, The Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Mononuclear cells were isolated by density gradient centrifugation and CD34+ cells were subsequently isolated using the MACS CD34 MicroBead kit and the autoMACS (Miltenyi Biotec, Amsterdam, The Netherlands). CB-MLL-AF9 (here mBlueberry2 ...
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bioRxiv - Cancer Biology 2019Quote: Half of each tumor was dissociated using the Human Tumor Dissociation Kit and Gentle MACS Tissue Dissociator with Heaters (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions prior to enumeration of live cells using trypan blue.
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bioRxiv - Immunology 2020Quote: ... The supernatant was removed and the pellet resuspended and processed as directed by the Multi-tissue Dissociation Kit I (Miltenyi Biotec, 130-110-201). Brains were enzymatically digested into a single cell suspension by rotating at 37°C for approximately 25 minutes with trituration halfway through incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Gt(ROSA26)SorCreERT2/+ mice as described (Hatsell et al., 2015)for tamoxifen regime using the Skeletal Muscle Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: Brain tissue samples including the cortex and hippocampus obtained from experimental mice that did or did not receive the mCRP treatment were gently dissociated into single-cell suspensions using the Adult Brain Dissociation kit (#130107677, Miltenyi Biotec, Auburn, CA, USA). Single-cell isolation and characterization of CD31+ cells were performed as previously described with a minor modification 10 ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected into single lobes before being dissociated into single cell suspension using gentleMACS octo-dissociator and Miltenyi lung dissociation kit (Miltenyi Biotec, 130-095-927). Red blood cells (RBCs ...
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bioRxiv - Bioengineering 2021Quote: ... and was then dissociated with an enzymatic and mechanical method using a MACS Neural Tissue Dissociation Kit for postnatal neurons (Miltenyi Biotec, 130-094-802) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Lung was collected in RPMI and was dissociated into single-cell suspension using the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) as per the company’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Fully de-identified human CD34 cells enriched blood samples were obtained from Bone Marrow and Transplantation Laboratory at UCSF and were MACS sorted using CD34 MicroBead Kit (Miltenyi Biotec, 130-046-702) and incubated with CD3 antibody (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Physiology 2023Quote: Single cardiomyocytes were isolated from the ventricles of C57BL/6J WT mouse neonates aged from postnatal day 0 to 3 by enzymatic and mechanical dissociation in a semi-automated procedure by using the Neonatal Heart Dissociation kit and the GentleMACS™ dissociator (Miltenyi Biotec, Gaithersburg, MD). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...