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Citations for Miltenyi Biotec :
1201 - 1250 of 1728 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 1 × 10^7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... + 10 U/μL DNase I DNase I (Roches)) or MACS Human Tumor Dissociation kit (DS_130-095-929, Miltenyi Biotec, Germany). The incubation was terminated when the digestive fluid turned turbid and the tissue block disappeared ...
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bioRxiv - Cancer Biology 2024Quote: ... hSS2 mouse tumors were harvested and digested to single cell suspension with the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). Approximately 5-10 million cells were crosslinked with 1% of formaldehyde ...
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bioRxiv - Biochemistry 2024Quote: We purified CD4+CD25-CD44lowCD62Lhigh naïve T cells obtained from the spleens of WT and Cars2+/– littermates with the Naïve CD4+ T Cell Isolation Kit II (130-093-227; Miltenyi Biotec) and an autoMACS Pro Cell Separator (Miltenyi Biotech) ...
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bioRxiv - Immunology 2024Quote: ... then mechanically dissociated in enzyme mix for 30 min at 37°C following the manufacturer’s protocol (Lung Dissociation Kit, gentleMACS Tissue Dissociator, Miltenyi Biotec). The single cell suspension was applied to a 70 µm filter with 2.5 mL Separation Buffer ...
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bioRxiv - Immunology 2024Quote: ... and negative magnetic selection was then used to purify CD4+ T cells (CD4 T cell isolation Kit, human, Miltenyi Biotec). Isolated CD4+ T cells were plated at a density of 0.8x106/ml in AIM media supplemented with CTS Immune Cell SR (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were magnetically labelled with Dead Cell Removal Microbeads from the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101). For 1.0x107 cells ...
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bioRxiv - Neuroscience 2021Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were minced with a scalpel and then enzymatically digested with a Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929, Germany) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2021Quote: ... dead cells were removed from our 24h activation sample (∼50% viability) using a MACS® Dead Cell Removal Kit (Miltenyi Biotec). In brief ...
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bioRxiv - Genomics 2020Quote: ... Brain slices were dissociated mechanically using a gentleMACS™ Octo Dissociator and enzymatically using the Adult Brain Dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were then filtered through 70-µm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... Para-tumour lung tissue samples from metastatic cancer or fibrosis patients were processed using a tumour dissociation kit (MACS Miltenyi Biotec) to isolate immune and epithelial cells ...
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...
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bioRxiv - Immunology 2019Quote: B220+ B cells were recovered from bone marrow mononuclear cells from WT or CCR7−/− mice by negative selection using a MACS microbeads isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... cells were purified with magnetic cell sorting (MACS) from healthy donor PBMCs using the CD1c (BDCA1) DC isolation kit (Miltenyi Biotec). To obtain a CD1c+CD14- population ...
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bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
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bioRxiv - Neuroscience 2020Quote: ... P1 and P2 were re-suspended in PBS and myelin was depleted from both fractions according to the instructions of a commercial myelin-removal kit (Miltenyi Biotec), before sorting MF-CA3 synaptosomes from myelin-depleted P1.
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bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Micro-dissected dentate gyri were cut into pieces with a scalpel blade and enzymatically dissociated using the Neural Tissue Dissociation Kit P (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were processed as previously described 54 and CD4+ T cells were enriched with the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... femurs of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... multiple femur of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... from control and CL- and cold-treated mice were collected after CL treatment and processed for SVC isolation using mouse adipose tissue dissociation kit (Miltenyi biotec) according to manufactures.
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD8a+ isolation kit (Cat no.-130-104-075) and Mini Separator Columns (Cat no.-130-042-201) were purchased from Miltenyi Biotec. H-2Kb OVA Tetramer-SIINFEKL-PE (Cat no.-TS-5001-1C ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation (Dakewe) and T cells were purified using the Pan T Cell Isolation Kit (Miltenyi Biotec), and stimulated with CD3/CD28 T cell Activator Dynabeads (Thermofisher ...