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Citations for Miltenyi Biotec :
1601 - 1650 of 1776 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2024Quote: ... human monocytes were isolated by negative selection with magnetic microbeads according to the manufacturer’s instructions (Pan Monocyte Isolation Kit) (Miltenyi Biotec, Bergisch Gladbach, Germany). A total of 3 x 106 monocytes/mL were seeded in complete RPMI medium in hydrophobic Lumox dishes (Sarstedt ...
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bioRxiv - Immunology 2024Quote: ... to obtain monocyte-derived macrophages which were purified with magnetic bead separation (CD14 negative isolation protocol, Monocyte Isolation Kit II. Miltenyi Biotec, Auburn, CA). Isolated CD14+ monocyte-derived macrophages (MDMs ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... we further isolated the CD34+ hematopoietic stem cells (HSCs) using magnetic-activated cell sorting according to the manufacturer’s instructions (CD34 MicroBead Kit, Miltenyi Biotec #130-046-702). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... and processed into a single cell suspension according to the manufacturers’ protocol using the adult brain dissociation kit (Miltenyi Biotec 130-107-677). Cells were Fc blocked (Biolegend #101320 ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: CD3+ T cells were enriched from the BM of 30 MM patients using the Pan T cell isolation kit with MS columns (both Miltenyi Biotec, Bergisch, Germany). 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector to target ratio ranging from 7.75:1 to 9:1 ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary NK cells were isolated from the spleens of mice (8 weeks old) by using the NK cell isolation kits according to the manufacturer’s instructions (Miltenyi Biotec #130-090-864). Primary NK cells were cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2023Quote: T2 and T3 cells were then depleted for CD45+ cells using the Miltenyi LD column prep kit (Miltenyi Biotec Inc., Charlestown, MA, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were collected from wistar rat at P5 and cortices and corpus callosum were dissected and dissociated using neural tissue dissociation kit (P) (Miltenyi Biotec, Paris, France) with gentle MACS Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Separations were performed on Control and EIF4G2KD HEC-1A cells using magnetic beads for CD133 (CD133 Microbead Kit, Miltenyi Biotec, 130-097-049), or CD44 (CD44 Microbeads Kit ...
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bioRxiv - Neuroscience 2024Quote: The assessment of expanded surface protein markers on sEV was carried out using a MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer’s protocol.44 Thirty seven distinct surface marker antibodies (CD1c ...
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bioRxiv - Cancer Biology 2024Quote: Mouse cells were depleted from the xenograft tumor to enrich for human cells using Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694) as previously described24 ...
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bioRxiv - Cancer Biology 2024Quote: Primary human monocytes were isolated from PBMCs from three donors using a negative selection classical monocyte isolation kit (Miltenyi Biotec 130-117-337) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were isolated using a CD34 MicroBead kit according to manufacturer’s instructions (magnetically activated cell sorting or MACS; Miltenyi Biotec, San Diego, CA). Pure populations of CD34+ HPCs were infected with TB40/E-WT or TB40/E-ΔUL138STOP HCMV (MOI = 2 ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cell Biology 2024Quote: CD34+ cells were purified from human cord blood (Cord Blood Bank of Beijing) using the MACS MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The two-phase erythroid differentiation protocol was modified from the previous study23.
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bioRxiv - Microbiology 2024Quote: ... CD34+ HSPC were isolated from human fetal liver obtained from the UCLA CFAR Gene and Cellular Therapy Core Laboratory (Los Angeles, California) or Advanced BioScience Resources, Inc (Alameda, CA) using a CD34+ microbead isolation kit (Miltenyi Biotec, Auburn, CA).
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bioRxiv - Immunology 2024Quote: ... naïve CD8+ T cells were purified by magnetic bead separation using mouse naïve CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-096-543) following vendor instructions ...
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bioRxiv - Immunology 2024Quote: ... Human primary NK cells were isolated from the cryopreserved PBMCs utilizing the NK Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-092-657) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... weighed and enzymatically digested using a Neural Tissue Dissociation Kit (130-092-628 for microglia and oligodendrocyte progenitors and neurons; 130-093-231 for astrocytes; Miltenyi Biotec, Germany Germany). Dissociated tissue was passed through a 100-μm cell strainer (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched for using the Miltenyi Mouse Naïve CD4+ T cell Purification Kit (Miltenyi Biotec, Cat. # 130-104-453) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Primary T cells were sorted from primary PBMC from healthy donors using Pan T cell isolation kit – human (Miltenyi Biotec, # 130-096-535). Isolated T cells were stimulated with plate-bound anti-CD3 and anti-CD28 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: PDC were depleted from PBMC by AutoMACS (deplete_s) after preincubation with Fc block and anti-CD304 microbeads (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). Total PBMC controls were subject to the same AUTOMACS protocol ...
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bioRxiv - Cell Biology 2024Quote: Each 60 mg portion of freshly obtained skeletal muscle was suspended in 1 mL ice-cold Lysis Buffer (Mitochondria Isolation Kit, human, 130-094-532, Miltenyi Biotec, CA, USA) containing 5 μL/mL Protease Inhibitor Cocktail (P8340 ...
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bioRxiv - Cancer Biology 2024Quote: ... before CD8+ T cells were isolated by negative selection using a CD8 T cell Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec; 130-104-075). Isolated CD8+ T cells were incubated in RPMI media supplemented with 10% FBS and stimulated for 1 hour with PMA (20 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: Tumours were dissected at endpoint and were processed into single-cell suspensions using a Mouse Tumour Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mechanically and enzymatically dissociated with fire-polished Pasteur pipettes and the neural tissue dissociation kit with papain (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Lamina propria fractions were prepared by enzymatic digestion and dissociation with the Lamina Propria Dissociation Kit and GentleMacs Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Dissociated cells were collected for use in qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Biotinylated RNA was incubated with constant rotation for 15 minutes at room temperature with μMACs streptavidin beads and was isolated using μMACs columns (μMACs Strepavidin Kit, Miltenyi Biotec 130-074-101) that had been previously equilibrated with wash buffer (100mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was obtained by mechanical resuspension followed by filtration through a 40-μm-pore-size cell strainer and dead cel;ls were removed using Dead Cell Removal Kit (Miltenyi Biotec 130-090-101). Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+ ...