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Citations for Miltenyi Biotec :
1451 - 1500 of 1776 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Primary BM mononuclear cells isolated from MM patients or healthy donors were enriched in CD138+ PCs using magnetic sorting with the CD138 Microbead Kit (Miltenyi Biotec). Cells were plated in 48-well plates previously seeded with human BM-derived mesenchymal cells.
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC were differentiated into cells of all three germ layers using the StemMACS Trilineage Differentiation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Genetics 2024Quote: ... were harvested and T cells were isolated by magnetic separation with the human Pan T Cell isolation kit and stimulated using TransAct (both Miltenyi Biotec). Cells were then cultured in TexMacs medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The lung was perfused with 1X PBS and processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). Blood was treated with RBC lysis buffer for five minutes ...
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bioRxiv - Cell Biology 2024Quote: ... CB mononucleated cells were enriched for CD34+ cells using the human CD34 MicroBeads Kit following manufacturer instructions (130-046-703, Miltenyi Biotec), assessed for purity (≥70% CD34+ cells) ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8+T cells in single-cell suspensions were purified from tumors using a magnetic cell sorting column purification system (CD8+ T Cell Isolation Kit, Miltenyi Biotec). The conditioned medium (CM ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from healthy donors by Ficoll gradient followed by primary CD4+ T-cell isolation by negative selection and magnetic separation using the human CD4+ T Cell Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tissue samples were then minced into fragments and enzymatically digested for 1 hour at 37°C into single-cell suspensions using the gentleMACS Octo dissociator and human tumor dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... mouse cell depletion was performed on whole bone marrow cells using the Mouse Cell Depletion Kit and LS columns (Miltenyi Biotec). RIPA lysis was performed with purified human cells and 25µg of protein were loaded on 10% Bis-Tris-Gel NuPAGE gels with MOPS SDS running buffer (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... the tumor was carefully dissected from the affected hemisphere and processed using the Adult Brain Dissociation Kit (130-107-677, Miltenyi Biotec) for digestion ...
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bioRxiv - Immunology 2024Quote: ... was used to lyse red blood cells before T cell isolation using the naive CD4+ T Cell Isolation Kit from Miltenyi Biotec or the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kits from BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and Tconvs (CD4+CD25-) were purified (90-95% pure) by using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: Blood and tonsil myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) were obtained by using CD1c+ Dendritic Cell Isolation Kit (Miltenyi Biotec) and the Diamond Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The remaining tissue was placed in a gentleMACS C-tube and processed using the gentleMACS Mouse Adipose Tissue Dissociation Kit and the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec). Cell suspensions were diluted to 107 cells/mL and kept on ice ...
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bioRxiv - Immunology 2024Quote: ... total CD4+ T cells or total CD8a+ T cells were isolated from WT or Aim2−/−mice using a magnetic cellular isolation kit (CD4+, Miltenyi Biotec, 130-104-454 ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... were isolated from spleens and lymph nodes of donor mice using magnetic cell separation (MACS) using mouse CD8a+ T cell isolation kits (Miltenyi Biotec), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-075), and 1 × 103 OT-I T cells in 100 mL of dPBS were transferred by retroorbital injection into recipient mice.
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ Tregs and CD4+CD25− T cells were purified from the peripheral LNs and spleen of Apoe−/− or Ccr4−/−Apoe−/− mice using a CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) and anti-CD4 beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and Flt3-Ligand (Bio-Techne) A fresh aliquot of donor cells was thawed and the MACS CD8+ T-cell isolation kit (Miltenyi Biotec) was used according to the manufacturers instruction to enrich for CD8 positive cells ...
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bioRxiv - Immunology 2024Quote: Tumors were mechanically dissociated with scissors and digested enzymatically using the Tumor Dissociation Kit mouse and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from whole blood by the ficoll density gradient method (CL5020) and CD4+ T cells were selected using the human CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... B and Treg cells were further isolated by negative selection using the mouse Pan T cell isolation kit II (130-095-130; Miltenyi Biotec), the mouse Pan B cell isolation kit II (130-104-443 ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting pellet was resuspended in 100 μl of microbeads from the dead cell removal kit (Miltenyi Biotec, 130-090-101) and processed according to the commercial protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... tumour fragments from the lymphatic melanoma and skin lesion to chest wall metastases were obtained respectively during the surgical procedure and dissociation was performed on the same day utilising the human tumour dissociation kit (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Different sample types were subjected to bead-based multiplex EV analysis by flow cytometry (MACSPlex EV IO Kit, human, Miltenyi Biotec). Unless indicated otherwise ...
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bioRxiv - Immunology 2024Quote: ... Human fetal thymus samples were HPCs were obtained from Ficoll-Hypaque–purified CB samples using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec), as described (38) ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor fragments were minced and enzymatically dissociated using a Miltenyi Human Tumor Dissociation Kit and Miltenyi gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Neuroscience 2024Quote: ... and each SEZ was cut into 5 or 6 small pieces of tissue and digested enzymatically using the Neural Tissue Dissociation Kit (T) in a GentleMACS Octo Dissociator with heating (Miltenyi Biotec). When the program was finished ...
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bioRxiv - Neuroscience 2024Quote: ... Then cortex and hippocampus parts were taken and dissociated into single cell suspension by using Adult Brain Dissociation Kit (Miltenyi Biotec) based on the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The flow through was collected and astrocytes were then acutely isolated using anti-ACSA-2+ (astrocyte cell surface antigen 2) Microbead Kit (Miltenyi Biotec). The flow through cell suspension was incubated in 15 µl of FcR blocking buffer for 10 min at 4°C followed by 15 µl of ACSA-2 microbeads for 10 min at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Neonatal ventricular cardiomyocytes (NRVM) were obtained by enzymatic dissociation of 1-day-old Wistar rats heart using Neonatal Heart Dissociation Kit (Miltenyi Biotec). Ventricles were cut into 1-2 mm pieces and incubated with digestive enzymes (37°C ...
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bioRxiv - Neuroscience 2024Quote: Cortices were dissected from P1 and P14 brains and dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628 for P1 and 130-094-802 for P14) ...
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bioRxiv - Cancer Biology 2024Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Cancer Biology 2024Quote: ... The remaining cell suspensions were then resuspended in DMEM medium and enriched with viable cells using the Dead Cell Removal Kit magnetic sorting protocol (Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: Surface protein profiling of primary and TERT EVs was performed via Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV IO Kit, human, 130-108-813, Miltenyi Biotec). EV-containing samples were processed as follows ...
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bioRxiv - Cancer Biology 2024Quote: Human monocytes were purified from buffy coats of healthy donors as reported elsewhere38 or using Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2020Quote: EpCAM-positive cells were isolated from the differentiated endodermal cells using a magnetic cell sorting kit (MACS; Miltenyi Biotec K.K. Cologne, Germany) with the CD326 (EpCAM ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: NK cells were purified from PBMCs from healthy donors using an NK cell isolation kit (Miltenyi Biotec, Inc., San Diego, CA, USA). NK cells were stimulated with irradiated (100 Gy ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...