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Citations for Miltenyi Biotec :
1501 - 1550 of 1758 citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Bone marrow aspirates from healthy donor and pathological patients were enriched for plasma cells by magnetic cell separation using a human CD138 positive selection and Macs Separator kits (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested spleens from intramuscularly vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Spleen Dissociation Kit (Miltenyi Biotec) with slight modifications to the manufacturer’s protocol ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested lungs from intranasally vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Lung Dissociation Kit (Miltenyi Biotec) according to manufacturer’s instructions including RBC lysis ...
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were magnetically purified using CD4+ T cell isolation kit and the autoMACS® Columns Pro Separator machine (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Lung tissue fragments were mechanically minced and dissociated with enzymes according to a Lung Dissociation Kit protocol (Miltenyi Biotec, 130-095-927). A Miltenyi Gentle MACS dissociator was used for mincing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... hiPSC-aCMs and -vCMs at day 20-30 post-differentiation were dissociated into single cells which were then enriched using a MidiMACS PSC-derived Cardiomyocyte Isolation Kit (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... We used a commercially available neural tissue dissociation kit for enzymatic dissociation following the manufacturer’s protocol (Miltenyi Biotec, San Diego, CA, USA). Specimens were subsequently washed with cold PBS ...
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bioRxiv - Immunology 2020Quote: ... Skin T cells from the breast and abdomen were isolated from punch biopsies following a standardized digestion protocol with the gentleMACS™ Dissociator and the whole skin dissociation kit containing enzyme A and D (both Miltenyi Biotec) before FACS-sorting for live (Hoechst 33258 negative or propidium iodide negative ...
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bioRxiv - Immunology 2019Quote: ... Bone-marrow (BM)-PMNs were isolated from the bone marrow using magnetic separation (mouse Neutrophil isolation kit, Miltenyi Biotec, 130-097-658) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: NK cells were purified by negative selection from mice splenocytes with an NK cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Granulocytes/neutrophils were isolated from the erythrocyte fraction by dextran sedimentation or from whole blood by immunomagnetic selection (130-104-434, MACSxpress® Whole Blood Neutrophil Isolation Kit; Miltenyi Biotec), and CD10neg/CD10pos neutrophils were separated by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Immunology 2022Quote: γδ T cells were sorted from the spleen of MCMV infected or control TCRα-/- mice using the TCR γδ T cell Isolation kit (Miltenyi Biotec). For γδ TCM and TEM sorting ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cancer Biology 2022Quote: ... Neutrophils and the other cell types were isolated from the suspension using the MACS Neutrophil isolation kit (Miltenyi Biotec, 130-097-658) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Epithelial cells were isolated from colon samples in the pre-digestion buffer using the Lamina Propria Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A purified monocyte fraction was isolated from the thawed cells using the negative selection MACS® Pan Monocyte Isolation Kit (Miltenyi Biotec) with LS columns according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... melanomas of α-SMAcreAtg5fl/fl and Atg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). RNA was extracted with the Macherey-Nagel NucleoSpin® RNA kit ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains from Control and Serpina3n cKO mice were processed using the Neural Tissue Dissociation Kit (P) (Cat# 130-092-628, Miltenyi Biotec, Germany) in conjunction with the gentleMACS Dissociator (Cat# 130-093-235 ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were cut into small pieces and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). Leukocytes were enriched by centrifugation with 40% Percoll ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic-activated cell sorting (MACS) with the CD34 Microbead Kit (catalog number #130-046-702, Miltenyi Biotec, Germany) and further purified by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Developmental Biology 2023Quote: ... single-cell suspensions were generated by dissociation of the organoids with a papain-based neural dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T cells were magnetically separated by negative selection with a Human Pan T cell isolation Kit (#130-096-535, Miltenyi Biotec, Germany) and stored long-term in Bambanker serum-free cell freezing medium (#BB01 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Lamina propria lymphocytes were isolated using a lamina propria dissociation kit in combination with a gentleMACS tissue dissociator according to the manufacturer’s instructions (Miltenyi Biotec, Gaithersburg, MD). In brief ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from RBC-depleted splenocytes using the Miltenyi CD8+ negative selection kit (Miltenyi Biotec, Cat # 130-104-075). Reporter-expressing CD8+ T cells were sorted using a BD Aria III ...
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bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cancer Biology 2023Quote: ... RBCs and debris of dead cells in single cell suspensions were respectively removed using Red Blood Cell Lysis Solution (10x) Kit (Miltenyi Biotec, Germany) and Dead Cell Removal Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissected from the limb and dissociated by shaking for 45 min at 37°C in an enzyme mix from the tumor dissociation kit (Miltenyi Biotec, 130096730). Cell suspension was then strained through a 40 µm filter ...
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bioRxiv - Microbiology 2023Quote: ... Neutrophils were isolated from 4 different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were then purified from the isolated PBMCs by negative selection using humanCD4+ T cell isolation kits (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Pineal tumors from IPDrosha/IPDicer1 mice were dissociated using the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec, cat. no. 130-095-942). Male and female NSG mice at 6-8 weeks of age were subcutaneously injected with 0.5-1.5 million tumor cells ...
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bioRxiv - Immunology 2024Quote: ... Resected tumor tissue not needed for diagnosis was digested using the Miltenyi Brain Tumor Dissociation kit (Miltenyi Biotec, cat. 130-095-42) and gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...