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Citations for Miltenyi Biotec :
1351 - 1400 of 1758 citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using magnet activated cell sorting (MACS) using the Pan T cell Isolation Kit (negative selection) according to the manufacturer’s instructions (Miltenyi Biotec, Germany). Following separation ...
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bioRxiv - Cell Biology 2020Quote: Single intestinal cells were obtained after enzymatic dissociation of the tunica muscularis using a Tumor Dissociation Kit (Miltenyi Biotec, Woking, UK), and YFP+ cells isolated using fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then negatively selected for CD45 and positively selected for CD117 (c-kit) using magnetic bead separation (Miltenyi Biotec). After selection ...
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bioRxiv - Immunology 2021Quote: ... and microglia was isolated with the Papain-based Neural Tissue Dissociation Kit (P) (# 130-092-628, Miltenyi Biotec B.V. & Co. KG) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with the LIVE/DEAD Fixable Aqua Dead Cell Staining kit (ThermofisherScientific) and acquired on a MACS Quant (Miltenyi Biotec) cytometer ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.
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bioRxiv - Immunology 2022Quote: ... brains were collected from P2-P5 pups and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628) on a gentleMACS Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... a region spanning the dorsal pallial midline (see Figure 1A) was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) on the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then immunoisolated with GFP-LC3B using 50 μL of GFP microbeads from a GFP isolation kit (Miltenyi Biotec; 130091125) added to each sample ...
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bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... tumors from unimmunized or mice immunized with WTL+LPS+PGPC were harvested then dissociated using the tumor Dissociation Kit and the gentleMACS dissociator (Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... ratio were purified by magnetic-activated cell sorting (MACS®) according to the manufacturer’s instructions (NK cell Isolation kit; Miltenyi Biotec). Total RNA from the samples was isolated using TRIzol® reagent following the manufacturer’s isolation protocol (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: Isolated Tregs from WT or NAC1-/- mice were activated and expanded with kits (# 130-104-454 and #130-095-925; Miltenyi Biotec), then treated with cycloheximide (150 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the mixture of unlabeled RNA and biotinylated RNA was incubated with streptavidin beads from uMACS Streptavidin kits (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Primary neurons were derived from the cerebral cortex of p0-p1 mice following standard operational procedure using the neural tissue dissociation kit-postnatal neurons (Cat. 130-094-802, Miltenyi Biotec) as previously described 30 ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Neuroscience 2022Quote: ... P10 and P20 were extracted from brains and dissociated using Adult Brain Dissociation Kit and OctoMACS Separator (both from Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were manually dissociated with a razor blade on ice and placed in the gentleMACSTM Octo Dissociator and Adult Brain Dissociation Kit (Cat# 130-107-677) (Miltenyi Biotec), supplemented with the inhibitor cocktail ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was cut into small pieces and processed using Neural Tissue Dissociation kit (P)-papain (Miltenyi Biotec 130-092-628) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... supernatants were discarded and cell pellets were processed according to the manufacturers protocol for MACS of astroglial cells using the ACSA2 isolation kit (Miltenyi Biotec). Afterwards ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with APC-eFluor780-conjugated anti-c-Kit (clone: 2B8) and fractionated using anti-APC magnetic beads and LS columns (both Miltenyi Biotec). c-Kit+ cells were then stained with combinations of antibodies as described in Supplemental Table 1 ...