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Citations for Miltenyi Biotec :
101 - 150 of 861 citations for 3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to electroporation.
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bioRxiv - Microbiology 2022Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to infection.
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). All HTLV-1 infected T-cell clones were maintained in 10 µM of the integrase inhibitor ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike peptides (PepTivator SARS-CoV-2 Prot_S, Miltenyi Biotec) were added at a final concentration of 0.6 nmol/ml in the presence of 1 μ (total cell culture volume ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Cancer Biology 2022Quote: ... and then blocked in FACS buffer (PBS, 2% BSA) containing FcR blocking reagent at 1:50 (Miltenyi Biotec) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... was homogenized in 1 ml PBS for 2 × 55 sec on a gentleMACS™ Octo Dissociator (Miltenyi Biotec) using a C-Tube (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were activated with 1 µg/ml PHA in presence of 10 ng/ml IL-2 (Miltenyi Biotec). PBMCs were mock- treated or infected with multiplicity of infection (MOI ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Neuroscience 2024Quote: ... and dissociated with enzyme mix 1 and 2 using gentleMACS™ Octo Dissociator (Miltenyi Biotec, 130-096-427). The dissociated cell suspension was applied to pre-wet 100 µm Cell Strainer (Falcon ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% B21 (Miltenyi biotec), 1% P/S (Sigma ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 200U/mL recombinant human IL-2 (rhIL-2, Miltenyi Biotec, Germany). Fresh complete RPMI medium supplemented with rhIL-2 was replaced every 6 days and T-cell lines were used for the experiments (i.e. ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... penicillin (100 U/ml) and interleukin 2 (IL-2, Miltenyi Biotec #130-097-745) (10 ng/ml) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-2 (rhIL-2, Miltenyi Biotec, Germany) with a an IL-10 secretion assay (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM CHIR99021 (Miltenyi Biotec), 0.5 μM A83-01 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/ml FGF8 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/mL FGF8 (Peprotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...
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bioRxiv - Pathology 2023Quote: ... GMCSF/IL-9 cytokine ratios of 1:2 diluted amniotic fluid were evaluated by the cytokine bead array (Miltenyi Biotec MACSPlex Cytokine 12 Kit ...
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bioRxiv - Systems Biology 2024Quote: ... tumor was cut into 1-2 mm pieces and transferred to a gentleMACS C Tube (Miltenyi Biotec #130-093-237) containing 4.7 mL of sterile media (Knockout DMEM/F12) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 ng/mL Fibroblast Growth Factor 2 (FGF-2) (Miltenyi Biotec, 130-105-787), for 14 days with medium change every 2 days ...
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bioRxiv - Microbiology 2024Quote: ... Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2020Quote: ... Cells were then washed three times and cultured with 1 ng/mL rhIL-15 or 20 IU/mL rhIL-2 (Miltenyi Biotec). After 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and incubated in 2% FBS in PBS with anti-ROR1-APC antibody (1:25, #130-119-860, Miltenyi Biotec) on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec). TCR tetramers were created by adding TCR to tetrameric AlexaFluor647-conjugated streptavidin (produced in-house ...
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...