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Citations for Miltenyi Biotec :
451 - 500 of 861 citations for 3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... intracellular staining for vGLUT1 (1/200, Miltenyi Biotec, #130-120-764 ...
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bioRxiv - Immunology 2023Quote: ... human TGFβ-1 (5 ng/ml, Miltenyi Biotec), anti-mouse IL-4 (11B11 ...
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bioRxiv - Molecular Biology 2024Quote: ... human CD11b (1/100 CD11b-APC; Miltenyi Biotec), human CD19 (1/100 CD19-BV510 ...
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bioRxiv - Molecular Biology 2024Quote: ... human CD45 (1/50 hCD45-APCvio770; Miltenyi Biotec). BM cells were also stained with human CD3 (1/50 CD3-APC ...
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bioRxiv - Molecular Biology 2024Quote: ... human CD15 (1/50 CD15-PE; Miltenyi Biotec), human CD11b (1/100 CD11b-APC ...
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bioRxiv - Microbiology 2024Quote: ... and 1:200 FcR blocking reagent (Miltenyi Biotec) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Synthetic Biology 2024Quote: ... the beads were removed and IL-2 was replaced with IL-7 and IL-15 (Miltenyi Biotec GmbH, 130-095-367 and 130-096-567) at 10 ng/ml each ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in 400 µl DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). Small samples of cells were fixed and air-dried on glass slides to control for cell purity by immunocytochemical staining of GLUL ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). After 24h or 48h ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:10 CD61-PE-Vio770 (Miltenyi Biotec, 130102627) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... CD11b (Clone M1/70, PE, 1:50, Miltenyi Biotec), Ly6G (clone 1A8 ...
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bioRxiv - Systems Biology 2020Quote: ... 1:10 Emb-PE (Miltenyi Biotec, 130-107-948). The staining was done in the dark at 4°C with agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% v/v BSA (Miltenyi Biotec, 130-091-376), 0.5% v/v Triton-X (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng/ml TGFβ (130-095-067, Miltenyi Biotec), 12.5 ng/ml IFN-γ (130-096-872 ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...
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bioRxiv - Cell Biology 2021Quote: ... and MEFSK4 (1:50 Miltenyi Biotec 130-120-802). This allows for the removal of CD11b cells which are known to express high levels of MBNL1 ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated with TransAct (1:150, Miltenyi Biotec) or PBS (negative activation control ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... and anti-c-myc-FITC (1:50, Miltenyi Biotec, cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec), 1× MEM NEAA (GIBCO™) ...
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bioRxiv - Systems Biology 2024Quote: ... 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec), 1× MEM NEAA (GIBCO™) ...
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bioRxiv - Molecular Biology 2024Quote: ... and CD34 (1/100 CD34-PE-Vio770; Miltenyi Biotec). Cells were analyzed by flow cytometry using the Novocyte analyzer (Agilent ...
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bioRxiv - Immunology 2024Quote: ... MEFSK4-PE (clone mEF-SK4, 1:100, Miltenyi Biotec), CD64-PE/Cy7 (clone X54-5/7.1 ...
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bioRxiv - Bioengineering 2024Quote: ... with 1% FcR blocking reagent (Miltenyi Biotec, Gaithersburg, MD) on ice ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... (Supp Fig.1) (Miltenyi Biotec, Inc., San Diego, CA) and were stimulated with anti-CD2/CD3/CD28 monoclonal Ab beads (Dynabeads ...
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bioRxiv - Cell Biology 2024Quote: ... CD31-APC (1:50) (130-111-354, Miltenyi Biotec), CD34-PE (1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody staining for FACS was performed in Flow Cytometry Staining Buffer (ThermoFischer Scientific) and following antibodies were used: PE-GLAST (ACSA-1, Miltenyi Biotec, 1/40), APC-CD133 (13A4 ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in cold selection buffer (PBS + 0.5% BSA + 2 mM EDTA) mixed with 20% (v/v) CD20 MicroBeads (Miltenyi Biotec #130-091-104 and #130-042-201) for 15 min at 4 ºC ...
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bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... MM cells were pelleted and resuspended in flow cytometry buffer (1× PBS with 1% FBS) then incubated with human FcR blocking reagent (Miltenyi Biotec, # 130-059-901) before staining with diverse fluorescent dye-conjugated monoclonal antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained for 30 min at 4 °C in the dark in PBS using the following antibodies and conjugates: qNSCs and aNSCs were discriminated by a combination of PE-conjugated anti-mGLAST (1:50, clone ACSA-1, Miltenyi Biotec, Bergisch Gladbach, Germany), APC-conjugated anti-mCD133 (1:75 ...