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Citations for Miltenyi Biotec :
1351 - 1400 of 1616 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was cut into small pieces and processed using Neural Tissue Dissociation kit (P)-papain (Miltenyi Biotec 130-092-628) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... supernatants were discarded and cell pellets were processed according to the manufacturers protocol for MACS of astroglial cells using the ACSA2 isolation kit (Miltenyi Biotec). Afterwards ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with APC-eFluor780-conjugated anti-c-Kit (clone: 2B8) and fractionated using anti-APC magnetic beads and LS columns (both Miltenyi Biotec). c-Kit+ cells were then stained with combinations of antibodies as described in Supplemental Table 1 ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: YFP+ Treg cells were isolated by sorting lymph node samples pre-enriched for CD25+ cells using a mouse CD25 Microbead kit (Miltenyi Biotec) on a FACSAria (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec; CAT#130-097-095) was used to purify T cells ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: EpCAM-positive cells were isolated from the differentiated endodermal cells using a magnetic cell sorting kit (MACS; Miltenyi Biotec K.K. Cologne, Germany) with the CD326 (EpCAM ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: NK cells were purified from PBMCs from healthy donors using an NK cell isolation kit (Miltenyi Biotec, Inc., San Diego, CA, USA). NK cells were stimulated with irradiated (100 Gy ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Bone marrow aspirates from healthy donor and pathological patients were enriched for plasma cells by magnetic cell separation using a human CD138 positive selection and Macs Separator kits (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested spleens from intramuscularly vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Spleen Dissociation Kit (Miltenyi Biotec) with slight modifications to the manufacturer’s protocol ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested lungs from intranasally vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Lung Dissociation Kit (Miltenyi Biotec) according to manufacturer’s instructions including RBC lysis ...
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were magnetically purified using CD4+ T cell isolation kit and the autoMACS® Columns Pro Separator machine (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Lung tissue fragments were mechanically minced and dissociated with enzymes according to a Lung Dissociation Kit protocol (Miltenyi Biotec, 130-095-927). A Miltenyi Gentle MACS dissociator was used for mincing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... hiPSC-aCMs and -vCMs at day 20-30 post-differentiation were dissociated into single cells which were then enriched using a MidiMACS PSC-derived Cardiomyocyte Isolation Kit (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... We used a commercially available neural tissue dissociation kit for enzymatic dissociation following the manufacturer’s protocol (Miltenyi Biotec, San Diego, CA, USA). Specimens were subsequently washed with cold PBS ...
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bioRxiv - Immunology 2020Quote: ... Skin T cells from the breast and abdomen were isolated from punch biopsies following a standardized digestion protocol with the gentleMACS™ Dissociator and the whole skin dissociation kit containing enzyme A and D (both Miltenyi Biotec) before FACS-sorting for live (Hoechst 33258 negative or propidium iodide negative ...
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bioRxiv - Immunology 2019Quote: ... Bone-marrow (BM)-PMNs were isolated from the bone marrow using magnetic separation (mouse Neutrophil isolation kit, Miltenyi Biotec, 130-097-658) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: NK cells were purified by negative selection from mice splenocytes with an NK cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Granulocytes/neutrophils were isolated from the erythrocyte fraction by dextran sedimentation or from whole blood by immunomagnetic selection (130-104-434, MACSxpress® Whole Blood Neutrophil Isolation Kit; Miltenyi Biotec), and CD10neg/CD10pos neutrophils were separated by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Immunology 2022Quote: γδ T cells were sorted from the spleen of MCMV infected or control TCRα-/- mice using the TCR γδ T cell Isolation kit (Miltenyi Biotec). For γδ TCM and TEM sorting ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cancer Biology 2022Quote: ... Neutrophils and the other cell types were isolated from the suspension using the MACS Neutrophil isolation kit (Miltenyi Biotec, 130-097-658) according to the manufacturer’s instructions ...