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Citations for Miltenyi Biotec :
1051 - 1100 of 2979 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Physiology 2021Quote: ... Dead cells were removed by using a dead cell removal kit and MS columns (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Single-cell RNA-seq libraries were made with the 10x Genomics Chromium Next GEM Single Cell 3’ Library Kit (v3.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2020Quote: ... Germinal center B cells were enriched by staining cells with GL7-PE followed with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec). Positive enrichment of GL7-expressing cells was performed using MACS LS columns (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... or for scRNAseq following an additional step of dead cell removal using the MACS Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... the cell suspension was loaded onto an LD column and αβTCR+ T cells were depleted by MACS cell separation per the manufacturer’s protocol (Miltenyi Biotec). After depletion ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were enriched magnetically by their expression of H2Kk using a MACSelect Transfected Cell Selection kit (Miltenyi Biotec, Sunnyvale, CA). Cas9-mediated indel formation at the Acvr1 or Bmpr1a locus was confirmed by T7E1 mismatch analysis on the enriched cell populations (T7E1 Nuclease – New England Biolabs ...
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bioRxiv - Immunology 2022Quote: The CD3+ cells and CD4 T cells were enriched from PBMCs using CD3 MicroBeads (Miltenyi biotec, #130-050-101, German) and CD4 MicroBeads (Miltenyi biotec #130-045-101 ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2022Quote: ... cells from lymph nodes and spleens of naïve OT-II or HNT transgenic mice were enriched for naïve cells by percoll gradients and CD4 T cells isolated by CD4 positive selection (Miltenyi Biotec) or using a CD4 naïve positive selection kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... first CD4+ T cells were isolated via positive selection using Magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). APCs were isolated with the same system via CD4 and CD3 negative selection ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Neuroscience 2022Quote: ... hRGC differentiation was monitored by tdTomato expression and cells were purified during days 45-55 using THY1.2 microbeads and magnetic activated cell sorting system (MACS, Miltenyi Biotec) as explained before19,22 ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 24h ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Immunology 2024Quote: T-cells were isolated from healthy donor PBMCs using the Pan T-cell Isolation Kit following manufacturer’s instructions (Miltenyi Biotec). Isolated T-cells were activated using anti-CD3/CD28 antibody-coated Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... red blood cells were eliminated using Red Blood Cell Lysis Solution (cat # 130-094-183, Miltenyi Biotec, Gaithersburg, MD, USA). These steps were followed by application of Debris Removal Solution ...
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bioRxiv - Cell Biology 2020Quote: ... Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... TAG1+ cells were isolated by MACS (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cell Isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with MACS buffer (Miltenyi Biotec) and stained with antibodies against CD40 (3/23 ...
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bioRxiv - Immunology 2022Quote: ... using magnetic-activated cell sorting (MACS, Miltenyi Biotec) according to manufacturer’s protocols and labeled with the proliferation dye CellTrace Violet (CTV) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in MACS buffer (Miltenyi Biotec) and incubated with antibodies to Ly6C conjugated to BV421 ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec); 36-48 h after activation ...
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bioRxiv - Immunology 2021Quote: ... and the magnetic cell sorter (autoMACS, Miltenyi Biotec). The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50 ...
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bioRxiv - Immunology 2020Quote: ... An additional dead cell removal step (Miltenyi Biotec) was conducted on hospitalized PBMC samples prior to aliquot for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ T cell isolation kits (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... B cell kit (Miltenyi Biotec, 130-090-862) was followed according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... the Direct Lineage Cell Depletion Kit (Miltenyi Biotec) was employed according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL stem cell factor (Miltenyi Biotec), 50 ng/mL vascular endothelial growth factor (GIBCO-PHC9394) ...
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bioRxiv - Cancer Biology 2023Quote: ... and a Dead Cell Removal kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dead Cell Removal Kit (Miltenyi Biotec, Germany) if needed ...
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bioRxiv - Developmental Biology 2023Quote: ... and a MiniMACS Magnetic Cell Separator (Miltenyi Biotec). Following dead cell removal ...
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bioRxiv - Molecular Biology 2023Quote: ... and red blood cell lysis solution (Miltenyi Biotec), respectively ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were analyzed with MACSQuant VYB (Miltenyi Biotec). For experiments involving 3-ORF SEMPER ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells treated with anti-FITC MicroBeads (Miltenyi Biotec) were magnetically separated via a MACS column (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... A dead cell removal kit from Miltenyi Biotec was employed after thawing ...
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bioRxiv - Immunology 2024Quote: ... Cells were also stained for NKG2D (Miltenyi Biotec). The PBMCs were then fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... the cell suspension was applied onto an LD column and αβ TCR-positive (αβ TCR+) T cells were depleted by Magnetic-Activated Cell Sorting (MACS) according to the manufacture’s protocol (Miltenyi Biotec).