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Citations for Miltenyi Biotec :
1251 - 1300 of 3164 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Immunology 2023Quote: NK cells were enriched from the spleens of C57BL/6 Kaede mice using NK Cell Isolation Kit (#130-115- 818, Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... CD34+ cells were purified from a portion of the cells using the CD34 Microbead Kit Ultrapure and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Part of the single-cell suspension was depleted of CD45+ cells to enrich for tumour cells using a CD45 MicroBeads kit (catalog no. 130-045-801; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Immunology 2023Quote: ... Lineage depletion was carried out before cell sorting by MACS Cell Separation and LS columns (Miltenyi Biotec, cat#130-042-401) to deplete CD3+ ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from wildtype mice spleens using CD8a+ T Cell Isolation Kit (130-104-075) from Miltenyi Biotec according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... following which the cells were cryopreserved at a concentration of 5×106-1×107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step.
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bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
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bioRxiv - Immunology 2023Quote: CD4 T cells were isolated from the spleens of donor mice and purified using the mouse CD4 T Cell Isolation Kit (Miltenyi Biotec). The purity and cell count of donor CD4 T cells were assessed using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.
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bioRxiv - Immunology 2023Quote: ... and the circulating CD45-PE+ cells were removed from the single cell suspension using magnetic sorting with anti-PE microbeads (Miltenyi Biotec) according to manufacturer’s recommendations (S1A Figure).
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... Cells were filtered through a 30μm cell strainer and incubated with CD90.2 magnetic beads (Miltenyi Biotec; Catalog no. 130-121-278) at 4°C for 10 minutes and then isolated using MACs LS (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... After dissociation the cell suspension was filtered through a 40 µm strainer and treated with red blood cell lysis solution (Miltenyi Biotec). Lastly ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were enriched by depletion of CD43+ cells with anti-CD43-conjugated magnetic beads (MACS anti-mouse CD43; Miltenyi Biotec). Resultant populations were >97% B cells based on B220 staining and FACS analysis ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100,000 cells were counted and the fraction of BFP and YFP positive cells was determined by flow cytometry (MACSQuant VYB, Miltenyi Biotec). The rate of change of the different strains is plotted as the natural log of the ratio of different strains at different time-points.
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bioRxiv - Immunology 2021Quote: ... On day4 transduced cells were positively selected by magnetic assisted cell sorting (MACS) via the cell surface antigen CD90.1 (Thy1.1) using microbeads (Miltenyi Biotec: 130-094-523); the manufacturer’s protocol was adjusted by using 4x less reagents and by maintaining cells at room temperature ...
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bioRxiv - Immunology 2020Quote: NK cells were purified from PBMCs from healthy donors using an NK cell isolation kit (Miltenyi Biotec, Inc., San Diego, CA, USA). NK cells were stimulated with irradiated (100 Gy ...
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bioRxiv - Developmental Biology 2019Quote: ... stained cells were passed through a 40-μm cell strainer and then collected over a MS column (Miltenyi Biotec, 130-042-201) in a magnetic field ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were magnetically purified using CD4+ T cell isolation kit and the autoMACS® Columns Pro Separator machine (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly cells were strained through a 70 μm cell strainer and then stained with CD11b (1:50 Miltenyi Biotec 130-113-800) and MEFSK4 (1:50 Miltenyi Biotec 130-120-802) ...
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bioRxiv - Immunology 2021Quote: NK cells were purified by negative selection from mice splenocytes with an NK cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic-activated cell sorting (MACS) with the CD34 Microbead Kit (catalog number #130-046-702, Miltenyi Biotec, Germany) and further purified by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Immunology 2023Quote: Fetal liver cells were processed into a single cell suspension and cKit-enriched using CD117 MicroBeads (Miltenyi Biotec, San Diego, CA, USA). The cKit-enriched population was stained with an antibody cocktail for surface markers of hematopoietic stem and progenitor cells ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were then purified from the isolated PBMCs by negative selection using humanCD4+ T cell isolation kits (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were separated by autoMACS Pro Separator (Miltenyi Biotec) with the program “posseld.” Cells in the flow-through fraction were washed and plated in a 6-well plate for 1 h in the medium supplemented with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 40 μm cell sieve (Miltenyi Biotec) and analyzed by flow cytometry (FACSaria III ...
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bioRxiv - Physiology 2019Quote: ... on an OctoMACS™Cell Separator system (Miltenyi Biotec). ATMs were cultured for 24h in RPMI-1630 supplemented with 10% FCS and 1% P/S ...
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bioRxiv - Immunology 2020Quote: CD8+ T cells were isolated by MACS (Miltenyi Biotec) from draining inguinal lymph nodes of MB49 bearing male and female mice 14 days post implantation (Fig ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were removed with a kit (Miltenyi Biotec, 130-090-101 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with FcR blocking Reagent (Miltenyi Biotec) and then stained with the appropriate combinations of mAbs and fluorochrome-conjugated streptavidin ...
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bioRxiv - Immunology 2021Quote: ... using an NK cell magnetic purification kit (Miltenyi Biotec). Murine NK were cultured in RPMI 1640 supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... strained through a 70 μm cell strainer (Miltenyi biotec), counted ...
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bioRxiv - Genetics 2022Quote: ... or Pan B Cell (Miltenyi Biotec, 130-104-433) isolation kits ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with CD45+ MACS beads (Miltenyi Biotec) and isolated by positive selection using LS columns (Miltenyi Biotec) ...