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Citations for Miltenyi Biotec :
901 - 950 of 954 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Neonatal ventricular cardiomyocytes (NRVM) were obtained by enzymatic dissociation of 1-day-old Wistar rats heart using Neonatal Heart Dissociation Kit (Miltenyi Biotec). Ventricles were cut into 1-2 mm pieces and incubated with digestive enzymes (37°C ...
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bioRxiv - Immunology 2024Quote: ... the cells were resuspended in 1 mL of MACS buffer and applied to LS columns (Miltenyi Biotec, Cat #: 130-042-401) for enriching tetramer-bound T cells ...
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bioRxiv - Immunology 2024Quote: ... The production of several major cytokines from CD4+ T cells was examined as described previously.30 Splenic CD4+ T cells (1×105 cells) isolated using MACS (Miltenyi Biotec) were stimulated with plate-bound anti-CD3 (10 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... with an end concentration of 1 µg/ml was added and the samples were measured in the MACSQant Analyzer 16 (Miltenyi Biotec). Cell Counts were analyzed with Flowlogic software.
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bioRxiv - Cancer Biology 2024Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Cancer Biology 2024Quote: Fresh spleen was collected in 3mL complete medium (CM; DMEM + 10%FBS + 1% peniciline/streptomycin) in a homogenization tube (gentleMACSTM C Tubes, Miltenyi Biotec). Spleen was dissociated using tissue dissociator gentleMACS Octo (gentleMACSTM ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed to dopaminergic patterning medium composed of CTS™ DMEM-F12 (with CTS™ N2 1:100) plus freshly added 200 ng/ml GMP Sonic Hedgehog (SHH, 130-095-727, Miltenyi Biotec), 100 ng/ml GMP FGF-8b (130-095-740 ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly cells were strained through a 70 μm cell strainer and then stained with CD11b (1:50 Miltenyi Biotec 130-113-800) and MEFSK4 (1:50 Miltenyi Biotec 130-120-802) ...
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bioRxiv - Cell Biology 2022Quote: ... 250 μl of each sample were incubated on ice for 1 h with 50 μl of α-GFP µMACS beads (Miltenyi Biotec). Immunoprecipitates were isolated using the μMACS GFP isolation kit ...
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bioRxiv - Microbiology 2022Quote: ... Tonsil and lung tissue samples were dissected to approximately 1 g pieces and placed into gentleMACS C Tubes (Miltenyi Biotec, Auburn, CA). The weight of each sample was recorded and PBS was added to a 10% weight/volume ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of each sample was transferred to an Eppendorf tube and assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). GFP-labeled B ...
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bioRxiv - Cell Biology 2024Quote: ... an equivalent of 1,000 human CD34+ cells from mBM and hOss were plated in 1 mL of methylcellulose-based medium (130-125-042, StemMACS™ HSC-CFU Assay Kit; Miltenyi Biotec) that contained human SCF ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Human myeloid CD1c+ DC were isolated from blood and kidney by positive immuno-magnetic selection using anti-CD1c/BDCA-1 microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) or on a FACS ARIA (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Immunology 2024Quote: The left lung was weighed and homogenized in 1 mL of RPMI supplemented with 10% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of microdissected vmPFC grey matter was homogenized in 2 ml of cold homogenization buffer (1M sucrose + 1% BSA dissolved in DEPC-treated water) using the benchtop gentleMACS™ Dissociator (Miltenyi Biotec, Germany) with the rotating paddle set to the Lung 02.01 program ...
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bioRxiv - Plant Biology 2024Quote: ... membranes were probed overnight at 4°C with the corresponding antibodies : anti-GFP-conjugated with HRP (Miltenyi Biotec 130-091-833, 1:5000), anti-RFP (Abcam 34767 ...
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bioRxiv - Immunology 2024Quote: PDC were depleted from PBMC by AutoMACS (deplete_s) after preincubation with Fc block and anti-CD304 microbeads (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). Total PBMC controls were subject to the same AUTOMACS protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...
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bioRxiv - Cell Biology 2024Quote: Each 60 mg portion of freshly obtained skeletal muscle was suspended in 1 mL ice-cold Lysis Buffer (Mitochondria Isolation Kit, human, 130-094-532, Miltenyi Biotec, CA, USA) containing 5 μL/mL Protease Inhibitor Cocktail (P8340 ...
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bioRxiv - Bioengineering 2021Quote: ... Lymph nodes were stained for CD19 expression using the panel described in supplemental table 1 following incubation with FcR blocking reagent (Miltenyi Biotec # 130-092-575). Cells were analyzed on a BD LSRFortessa ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were cultured for one week and then the same procedure was followed with anti-Prominin-1 MicroBeads (Miltenyi Biotec, Cat# 130-092-564) for the negative selection of radial glia ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was replaced with a neural maintenance medium (NMM) supplemented with 1 µM StemMACS™ LDN-193189 (Miltenyi Biotec, #130-106-540) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Plant Biology 2022Quote: ... isolated from transfected protoplasts were separated by SDS-PAGE and subjected to immunoblotting using an anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000).
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were cut into smaller pieces and transferred into Enzyme Mix 1 (955 μl of Buffer Z, 25 μl of Enzyme P [Miltenyi Biotec, 130-094-802]) in a 1.5-ml tube ...
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bioRxiv - Immunology 2023Quote: ... cells were transduced with 2uL per well of concentrated Lenti-CRISPR-v2-GFP supernatant (transduction efficiency kept <20% to promote single copy integration) and 1uL Vectofusin-1 (1X, Miltenyi Biotec, 130-111-163) diluted in 50 uL of cord-blood optimized media ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellet was gently tapped to loosen it and then resuspended in 1 mL of wash buffer containing 50 μL of CD43 (Ly-48) MACS microbeads (Miltenyi Biotec, #130-049- 801). The mixture was incubated on ice for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... to label and exclude islet cells, HPx1-Dylight 488 (Novus, NBP1-18951G) to label acinar cells and CD133/1(AC141)-PE (Miltenyi Biotec 130-080-801), CD133/2(293C3)-PE (Miltenyi Biotec 130-090-853 ...
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bioRxiv - Developmental Biology 2024Quote: ... was added to the bottom of the plate and cultured in the organoid culture medium 1 (50% DMEM/F12, 50% MACS Neuro Medium (Miltenyi Biotec, 130-093-570), 1 mM MEM non-essential amino acids ...
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bioRxiv - Cell Biology 2021Quote: ... The hiPSC-CMs were incubated with the conjugated antibodies cTnT-Vioblue or cTnT-FITC (1:50, Miltenyi Biotec, #130-120-402 or #130-119-575). All antibodies were diluted in permeabilization medium (medium B ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Genomics 2024Quote: ... the cytotrophoblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin (APC)-conjugated anti-human leukocyte antigen (HLA)-ABC antibody (APC-anti-HLA-ABC, Miltenyi Biotec, #130-101-467, lot #5191002371). The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR ...
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bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR) antibody (APC-anti-EGFR, Miltenyi Biotec, #130-110-529, lot #5190107002). Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec ...