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Citations for Miltenyi Biotec :
801 - 850 of 954 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Plant Biology 2020Quote: ... 30 μg was detected with horseradish peroxidase (HRP)-conjugated antibodies against GFP (Anti-GFP-HRP, 1:10000, Miltenyi Biotec) or anti-tubulin (mouse monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Neonatal murine microglia were isolated from P0 CD-1 pups using CD11b microbeads (Ca #130-093-634, Miltenyi Biotec) and their purity was assessed by immunocytochemistry of myeloid cell markers (Iba-1 and CD11b ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated with the surface antibody CD34-PE-Vio770 (clone AC136, lot #5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP [GG4-2C2.12.10] (1:5000; 130-091-833; Miltenyi Biotec), rabbit anti-EXOC1 antibody (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×107 cells per replicate were purified using 100ul of Pluripotent Stem Cell microbeads (Miltenyi Biotec 130-095-804), and LS columns (Miltenyi Biotec 130-042-401) ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in MACS buffer (0.5% BSA and 1 mM EDTA in PBS, pH 7.4) and incubated with CD11b MicroBeads (Miltenyi Biotec) for 30 minutes to label the microglial cells ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was removed and 1 mL of wash buffer (PBS + 0.5% BSA (Miltenyi Biotec, Cat. # 130-091-376) + 1 U/uL of RNase inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... antibody labeling and boosting step was performed with the addition of CD11b (1:50) (Miltenyi Biotec, 130-110-611) and GFP-Nanobooster LOT 102 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... added with 40μL of magnetic microbeads conjugated with anti-Thy-1 antibody (Miltenyi Biotec 130–049–101, Auburn, CA), and mixed well ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
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bioRxiv - Genomics 2023Quote: ... TB6505 and TB6545 (1 givinostat and 7 vehicle slices) were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) with modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Overnight cultures were diluted in 1:100 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells11 ...
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bioRxiv - Genomics 2024Quote: ... 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot no. 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were by counter-staining with anti-CD4-APC antibody (1:100) using flow cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Immunology 2024Quote: CD8 T cells were isolated from spleens of OT-1 mice using the CD8 mouse isolation kit (Miltenyi Biotec) according to the instructions of the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were plated and then treated 24 h later with 0.1 µM and 1 µM Taxol or 0.1% DMSO in the presence of propidium iodide (Miltenyi Biotec). The plate was incubated in an IncuCyte Live Cell Analysis System (Essen Bioscience) ...
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bioRxiv - Cancer Biology 2020Quote: ... for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... The perfused brains were dissected and cut into small 1 mm3 pieces before homogenizing them in DPBS++ containing 0.4% DnaseI on the tissue dissociator (Miltenyi Biotec) at 37 °C for 35 mins ...
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bioRxiv - Cell Biology 2021Quote: ... The high pluripotent stem cells form colonies were selected by Anti-Tra-1-60-PE and Anti-PE MicroBeads following the manufacturer’s instructions (MACS Miltenyi Biotec). The stable colonies were frozen down at -80°C ...
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bioRxiv - Systems Biology 2020Quote: ... Cell suspensions (up to 1×108 cells/500 µL of Magnetic Bead Buffer) were applied onto MS columns (Miltenyi Biotec) in a magnetic field ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Genetics 2023Quote: ... and the precipitated pellet was resuspended in 2.5 mL 1× Buffer and filtered with a 70 µM MACS SmartStrainer (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
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bioRxiv - Microbiology 2024Quote: ... they were stained with 100 μl of PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770 ...
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bioRxiv - Immunology 2024Quote: ... conjugate and staining information are as follows: anti-human CD3 (Miltenyi Biotec, Clone BW264/56, APC-R700, 1:200, 4℃); anti-human CD19 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of 0.2% Tween-20/1X PBS containing 1 μL of PE-Foxp3 (Miltenyi Biotec cat# 130-111-678) was added and cells were incubated for 30 min in the dark ...
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bioRxiv - Genetics 2024Quote: ... 1 × 10^7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... then washed with 3 mL washing buffer (PBS + 1% BSA + 0.2 U/μL RNase inhibitor) and filtered through 30 μm MARC SmartStrainer (Miltenyi Biotec) twice ...
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bioRxiv - Cell Biology 2024Quote: ... Lineage depletion (B220, CD3, Gr-1, and Ter119) was performed prior to sorting using MACS magnetic microbead kits (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were further incubated overnight at 4°C with anti-PD1 antibody (Miltenyi Biotec, 130-117-384; 1:100) and anti-CK antibody (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...