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Citations for Miltenyi Biotec :
901 - 950 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and Streptavidin MicroBeads (Miltenyi BioTec, 130-048-102) per manufacturer.s instructions ...
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bioRxiv - Immunology 2024Quote: ... then mechanically dissociated in enzyme mix for 30 min at 37°C following the manufacturer’s protocol (Lung Dissociation Kit, gentleMACS Tissue Dissociator, Miltenyi Biotec). The single cell suspension was applied to a 70 µm filter with 2.5 mL Separation Buffer ...
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bioRxiv - Immunology 2024Quote: ... mouse (Miltenyi Biotec 130-052-301).
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells isolated from spleen and lymph nodes were purified by incubating with anti-CD4 and CD8 microbeads (Miltenyi Biotec) and passing through LS magnetic columns following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Dead cells were removed from culture using the Dead Cell Removal Kit (Miltenyi Biotec, cat. 130-090-101). Then ...
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bioRxiv - Immunology 2024Quote: ... human (Miltenyi Biotec, cat. 130-045-901) and prepared for cell sorting exactly as described (Weymar et al. ...
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bioRxiv - Immunology 2024Quote: ... using a gentleMacs dissociator (Miltenyi Biotec). The lungs were then incubated for 30 min at 37°C and then further homogenized a second time with the gentleMacs ...
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bioRxiv - Immunology 2024Quote: ... BDCA-4-magnetic beads (MACS Miltenyi Biotec), LS Columns (MACS Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Purification of primary pDC was performed using the Plasmacytoid Dendritic Cell Isolation Kit II (#130-097-415 Miltenyi Biotec), reaching >93% of purity ...
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bioRxiv - Immunology 2024Quote: ... anti-IFNγ (Miltenyi Biotec)) +/-APC (Cambridge ProteinWorks ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Immunology 2024Quote: ... and negative magnetic selection was then used to purify CD4+ T cells (CD4 T cell isolation Kit, human, Miltenyi Biotec). Isolated CD4+ T cells were plated at a density of 0.8x106/ml in AIM media supplemented with CTS Immune Cell SR (Gibco ...
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bioRxiv - Immunology 2024Quote: ... followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... streptavidin microbeads (Miltenyi Biotec), and LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... B cells were enriched by incubating cells with anti-CD43-biotin (S7) and magnetic enrichment with LS columns (Miltenyi Biotec). For class switching to IgG1 ...
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bioRxiv - Immunology 2024Quote: ... and LS columns (Miltenyi Biotec). FACS was performed on a FACSAria Fusion (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... following the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). A 200 µL portion of each lung digest was designated for lung fungal burden assessment ...
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bioRxiv - Immunology 2024Quote: ... mouse (Miltenyi Biotec, Bergish Gladbach, Germany), according to manufacturer’s directions ...
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bioRxiv - Immunology 2024Quote: ... and PE anti-HER2 (Miltenyi Biotec, Bergisch Gladbach, Germany). PE ...
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bioRxiv - Immunology 2024Quote: ... bone marrow was harvested from donor mice and enriched for neutrophils using a Neutrophil Isolation Kit (Miltenyi Biotec). Isolated cells were labeled with Cell Trace CFSE or Violet Proliferation Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Labelled and unlabelled cells were then again separated by MACS (Miltenyi Biotec).
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were isolated by MACS using the human CD14+ isolation kit (Miltenyi Biotec). CD14+ monocytes were differentiated into DCs over 6 days by culturing in RPMI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and pDCs were positively selected from PBMCs using BDCA-4-magnetic beads (MACS Miltenyi Biotec), as previously described (Decembre et al. ...
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bioRxiv - Immunology 2024Quote: ... LS Columns (MACS Miltenyi Biotec) and bottle top vacuum filters 0.22 μm (Nalgene) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified by incubation with anti-CD4 microbeads and passing through a magnetic column (Miltenyi Biotec). The purity of cells was higher than 95% as determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: Primary CD4+ T cells were enriched from healthy donor cryopreserved PBMCs by negative selection (CD4+ T Cell Isolation Kit human, Miltenyi Biotec, cat. 130-096-533) and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3 ...
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bioRxiv - Molecular Biology 2024Quote: ... CD271 and CD133 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2024Quote: ... The sorting was performed on the basis of the manufacturer’s instructions (Miltenyi Biotec) with minor adjustments ...
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bioRxiv - Molecular Biology 2024Quote: ... The CD34+ cell fraction was then isolated from the MNCs using the MidiMACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were isolated using a CD34 MicroBead kit according to manufacturer’s instructions (magnetically activated cell sorting or MACS; Miltenyi Biotec, San Diego, CA). Pure populations of CD34+ HPCs were infected with TB40/E-WT or TB40/E-ΔUL138STOP HCMV (MOI = 2 ...
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bioRxiv - Microbiology 2024Quote: ... which were isolated from cord samples collected from full term deliveries (Miltenyi Biotec CD133 MicroBead Kit, Human). Human CD45+ levels were assessed at 13-16 weeks post transplantation (wpt ...
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bioRxiv - Molecular Biology 2024Quote: ... and mean fluorescence intensity (MFI) was measured (MACSQuant Analyser 10, Miltenyi Biotec, Germany). As a control ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s instructions and analysed the samples with MACSQuant Analyzer 10 (Miltenyi Biotec, Germany). In short ...
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Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spreadbioRxiv - Microbiology 2024Quote: ... and 1% penicillin-streptomycin in gentleMACS™ M tubes (Miltenyi Biotec) to generate a 10% tissue homogenate.
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bioRxiv - Neuroscience 2024Quote: ... tissues were enzymatically digested in the gentle MACS dissociator (Miltenyi Biotec, #130-093-235) for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... single cell dissociation was performed using the Adult brain dissociation kit (Miltenyi Biotec, #130-107-677) according to the manufacturer’s instructions of the single cell dissociation kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Bone marrow progenitors (c-Kit+ cells) were selected using the CD117 MicroBead Kit (Miltenyi Biotec) and transduced with the pMYs-IRES-GFP vector ...
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bioRxiv - Molecular Biology 2024Quote: ... we used a commercial multiplex bead-based flow cytometry kit (MACSPlex Exosome Kit, human, Miltenyi Biotec, Germany) according to the manufacturer’s instructions and analysed the samples with MACSQuant Analyzer 10 (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... Using the MACS Mouse Cell Depletion Kit (Miltenyi Biotec), cells were labelled according to manufacturer’s instructions and sorted by depletion of murine hepatocytes using the autoMACS Pro Separator ...
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bioRxiv - Molecular Biology 2024Quote: ... The muscle slurry was filtered through 70 µm (352350, Falcon) and 30 µm (130-098-458, Miltenyi Biotec) cell strainers to remove large pieces of tissue and cell debris ...
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bioRxiv - Microbiology 2024Quote: ... The harvested lungs were homogenized in 4.5 ml of distilled water with the gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Bergish Gladbach, Germany) and the homogenates were plated on Middlebrook 7H10–oleic acid ADC (7H10-OADC ...
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bioRxiv - Microbiology 2024Quote: ... Single plane illuminated (light-sheet) image stacks of cleared lung lobes were acquired using a Miltenyi-LaVision Biotec Ultramicroscope II Light Sheet system (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) equipped with Olympus MVX-10 Illumination microscope body and MV PLAPO 2XC (Olympus ...
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bioRxiv - Bioengineering 2024Quote: ... with gentleMACS™ Perfusers (Miltenyi Biotec, 130-128-151), gentleMACS™ Perfusion Sleeves (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... and Liver Perfusion Kit (Miltenyi Biotec, 130-128-030). For the liver immune cell flow cytometry analysis ...
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bioRxiv - Bioengineering 2024Quote: ... primary hepatocytes and hepatic non-parenchymal cells (NPCs) were isolated using the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427) with gentleMACS™ Perfusers (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... liver tissues from the ALF model were dissociated using the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... gentleMACS™ Perfusion Sleeves (Miltenyi Biotec, 130-128-752), and Liver Perfusion Kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... at 37 ℃ utilizing gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Dissociated liver tissues were filtered through 70 μm cell strainers (SPL ...
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bioRxiv - Bioengineering 2024Quote: ... The livers were transferred to gentleMACS™ C Tubes (Miltenyi Biotec, 130-096-334) and dissociated into single-cell suspension with DMEM containing DNase (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... 5 × 106 CD4+ cells were purified from splenocytes obtained from WT and Cars2+/– female mice by using murine CD4 (L3T4) Microbeads (130-117-043; Miltenyi Biotec), and cells were transferred into Rag2-/- recipients on day 0 ...