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Citations for Miltenyi Biotec :
751 - 792 of 792 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Immunology 2024Quote: ... naïve CD8+ T cells were purified by magnetic bead separation using mouse naïve CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-096-543) following vendor instructions ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched for using the Miltenyi Mouse Naïve CD4+ T cell Purification Kit (Miltenyi Biotec, Cat. # 130-104-453) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Tumours were dissected at endpoint and were processed into single-cell suspensions using a Mouse Tumour Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
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bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and efficiently depleted of non-target cells by addition of magnetic microbeads conjugated to mouse anti-biotin monoclonal IgG1 antibodies and passage through LS ferromagnetic columns (Miltenyi Biotec, 130-042-401). The flow through ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Immunology 2024Quote: ... CD4 4+ T cells and total T cells were purified from cell suspensions using Mouse CD4+ T cell and total T cell Isolation Kits from Miltenyi Biotec (Auburn, CA, USA) (Supplemental material) ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
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bioRxiv - Cell Biology 2024Quote: ... Flowthrough was then spun down and resuspended in MACS buffer and incubated with blocking buffer and anti-ACSA2 mouse magnetic microbeads (Miltenyi Biotec, 130-097-678) for 30 minutes at 4°C with rotation to isolate astrocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... forelimb and hindlimb muscles from P4 Poglut1-cKO and sibling controls were dissociated using the mouse Skeletal Muscle Dissociation Kit and the gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Immunology 2023Quote: Spleens from four- to six-week-old mice were processed to single cell suspensions and CD4+ T cells were enriched using mouse CD4 (L3T4) Microbeads kit (Miltenyi Biotec Cat # 130-117-043) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: Murine nCMs were isolated from the ventricles of 1-2 days old wildtype C57BL/6 and Lmnaflox/flox mouse pups using MACS neonatal heart dissociation kit according to the manufacturer (Miltenyi Biotec cat# 130-098-373). Following digestion ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Cancer Biology 2024Quote: ... Mouse prostate tumor and normal tissues were dissociated for single cell RNA sequencing using the Tumor Dissociation kits from Miltenyi Biotec (cat. no. 130-096-730). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...