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Citations for Miltenyi Biotec :
701 - 750 of 792 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Immunology 2024Quote: ... The lung was perfused with 1X PBS and processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). Blood was treated with RBC lysis buffer for five minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL of each sample was removed and set aside as representative “crude mitochondrial fraction.” The remaining 300 μL was diluted to 2 mL total with ICB and combined with 60 μL of superparamagnetic microbeads coated with mouse IgG1 antibodies against GFP (#130-091-125; Miltenyi Biotec). These samples were then placed on a rotating wheel at +4°C for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting single cell suspension was incubated with anti-mouse CD11b (i.e., microglia-specific) magnetic MicroBeads (Miltenyi Biotec, #130–093–634) and microglia were positively selected for via column purification (Miltentyi Biotec ...
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bioRxiv - Immunology 2024Quote: Control or DON-treated tumors (n = 1 per group) were harvested after 6 days of treatment and positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) before submission ...
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bioRxiv - Immunology 2024Quote: ... The remaining tissue was placed in a gentleMACS C-tube and processed using the gentleMACS Mouse Adipose Tissue Dissociation Kit and the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec). Cell suspensions were diluted to 107 cells/mL and kept on ice ...
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bioRxiv - Immunology 2024Quote: ... were isolated from spleens and lymph nodes of donor mice using magnetic cell separation (MACS) using mouse CD8a+ T cell isolation kits (Miltenyi Biotec), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Tumors were mechanically dissociated with scissors and digested enzymatically using the Tumor Dissociation Kit mouse and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... B and Treg cells were further isolated by negative selection using the mouse Pan T cell isolation kit II (130-095-130; Miltenyi Biotec), the mouse Pan B cell isolation kit II (130-104-443 ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution; 130-113-806, Miltenyi Biotec) and anti-mouse CD45-APC (1.15:100 dilution ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were enriched by depletion of CD43+ cells with anti-CD43-conjugated magnetic beads (MACS anti-mouse CD43; Miltenyi Biotec). Resultant populations were >97% B cells based on B220 staining and FACS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were subjected to a CD45+ enrichment using anti-CD45 microbeads (mouse: 130-052-301, human: 130-045-801, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng ml−1 mouse recombinant vascular endothelial growth factor and 20 ng ml−1 human recombinant fibroblast growth factor-2 (all from Miltenyi Biotec) as described 85 and plated on pre-coated (Collagen I (10 µg ml−1 Millipore) ...
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bioRxiv - Bioengineering 2024Quote: Hemolysates were prepared from either erythroid cell after 18 days of differentiation for in vitro differentiation experiments or human erythroid cells directly purified from xenotransplanted mouse BM by CD235a microbeads (130-050-501, Miltenyi Biotec). For single-chain globin variants ...
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bioRxiv - Cancer Biology 2024Quote: Tumors from mouse mammary-fat pad (MFP) were harvested and placed in MACS Tissue Storage Buffer (Miltenyi Biotec, 130-100-008) on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Cortical cells were then cleared from microglia by incubating with anti-CD11b human/mouse magnetic microbeads (Miltenyi Biotec, 130-093-634) for 10 minutes at 4°C with rotation and passed through an LS separation column (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2024Quote: Isolation of satellite cells from Poglut1-cKO mouse skeletal muscle was performed as described by the manufacturer with minor modifications (#130-098-305, #130-104-268; Miltenyi Biotec). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Then 1.5x 105 cells/cm2 in 24-well dishes were seeded and mouse receptor activator of nuclear factor kb ligand (RANKL, Miltenyi Biotec, Germany) was added at the final concentration of 35 ng/ml to initiate osteoclasts (OC ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells from MLNs of Hpb cured VillinCre-A2BARfl/fl and corresponding controls VillinCre infected mice were magnetically sorted from MLN and spleen single cell suspensions using mouse CD4 MicroBeads (Miltenyi Biotec, Inc.) sorted on positive selection columns ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were cut into small pieces and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). Leukocytes were enriched by centrifugation with 40% Percoll ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... melanomas of α-SMAcreAtg5fl/fl and Atg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). RNA was extracted with the Macherey-Nagel NucleoSpin® RNA kit ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Immunology 2024Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Immunology 2024Quote: ... wild-type CD4+ T cells were isolated using the Miltenyi Mouse CD4+ T cell isolation kit (Miltenyi Biotec, Cat. # 130-104-454) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD4+ naïve T cells were purified using the naïve CD4+ T cell Isolation kit for mouse from Miltenyi Biotec (130-104-453). For short term kinetics 10×106/ml naïve CD4 T cells were stimulated with 1µg/ml of α- CD3 and α-CD28 for the indicated time points ...
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bioRxiv - Immunology 2024Quote: ... cells were pre-enriched using the “Pan B Cell Isolation Kit II mouse” enrichment kit from Miltenyi Biotec (ref. 130-104-443) in which we added a biotinylated anti-IgD antibody for further enrichment of IgDneg B cells (Biolegend ref ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells from cord blood or adult donors were sorted using microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (Miltenyi Biotec MACS) through positive magnetic bead selection on LS columns.
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... excised tumors (200 mg) were dissociated in RPMI media containing enzyme cocktail mix from the mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA) and placed into gentleMACS dissociators per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...