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Citations for Miltenyi Biotec :
751 - 800 of 1548 citations for Mouse Anti Crimean Congo Hemorrhagic Fever Virus Gn Protein BH4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Cell Biology 2024Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash three times with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
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bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Each mouse was sacrificed at day 17 to collect feces and intestinal sections all homogenized in PBS using gentleMACSTM OCtoDissociator (Miltenyi Biotec) and plated on both Drigalski plates and LB plates overlayed with strain Mt1B1 ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Cancer Biology 2020Quote: ... After two weeks of treatment the mice were euthanized and the lungs were dissociated according to the kit instructions (Mouse Lung Dissociation Kit, MACS Miltenyi Biotec) and the cells were sorted for the GFP positive fraction ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Cell Biology 2019Quote: ... For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770, 130-104-224, Miltenyi Biotec), CD105 (APC ...
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bioRxiv - Cancer Biology 2020Quote: ... femurs of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... multiple femur of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... from control and CL- and cold-treated mice were collected after CL treatment and processed for SVC isolation using mouse adipose tissue dissociation kit (Miltenyi biotec) according to manufactures.
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Genetics 2022Quote: ... kidneys were quickly dissected after mouse euthanization and transferred to gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in 2ml of digestion buffer (13.5 ml of Dispase (STEMCELL ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed and CD11c+ dendritic cells were enriched using CD11c MicroBeads UltraPure (mouse) (Miltenyi Biotec, #130-125-835).
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bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
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bioRxiv - Cancer Biology 2021Quote: ... live CD45− cells were isolated by negative selection from digested WT and CCN4-KO tumors using the mouse CD45 (TIL) MicroBeads kit (Miltenyi Biotec). A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... whole bone marrow (WBM) cells isolated by crushing femur and tibia from each mouse were incubated with 1x RBC Lysis Solution (Miltenyi Biotec) to eliminate red blood cells ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T-cells were isolated from lymph nodes and spleens of OT-II TCR transgenic mice using negative selection with a mouse CD4+ T Cell Isolation Kit (Miltenyi Biotec). Splenocytes from C57BL/6 mice were T cell depleted by complement lysis of Thy1.2+ cells (clone J1J ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: Mouse spleens and lymph nodes were removed from RNAlater solution and transferred to gentleMACS M tubes (Miltenyi Biotec 130-093-236) and 2 ml microtubes (OMNI 19-629-3 ...
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: YFP+ Treg cells were isolated by sorting lymph node samples pre-enriched for CD25+ cells using a mouse CD25 Microbead kit (Miltenyi Biotec) on a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Cell Biology 2023Quote: ... Three independent replicates of Epcam-positive kidney epithelial cells (control kidney, CK) were MAC-sorted from freshly isolated single cell suspensions from whole mouse kidneys using microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Magnetic enrichment was carried out on an AutoMACS using microbeads conjugated to antibodies recognizing biotin or mouse CD117 (all from Miltenyi Biotec). HSCs (CD45.2+ ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor pieces were dissociated using a modified protocol based on Miltenyi (mouse) Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...