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Citations for Miltenyi Biotec :
651 - 700 of 1548 citations for Mouse Anti Crimean Congo Hemorrhagic Fever Virus Gn Protein BH4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Bioengineering 2023Quote: ... the yeast cells were washed once with PBSB and incubated with 750 μL of Protein A microbeads (Miltenyi Biotec, 130071001) per 109 cells ...
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bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...
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bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
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bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...
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bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD25+CD127low cells were isolated from a healthy donor and expanded for 21 days using anti-CD3 and anti-CD28-coated beads (Miltenyi Biotec) and 300 units/mL rhIL-2 (Chiron) ...
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bioRxiv - Immunology 2021Quote: ... WKy CD4+CD25high cells were isolated by magnetic and fluorescence-activated cell sorting and expanded with anti-CD3 and anti-CD28 coated beads (Miltenyi Biotec) and 1000 units/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: TCR-deficient J.RT3-T3.5 cells were transduced with MEL5 TCR lentiviral particles and magnetically enriched using anti-murine TCRβ–PE (clone REA318) in conjunction with anti-PE MicroBeads (Miltenyi Biotec). MEL5 TCR+ J.RT3-T3.5 cells were then transduced with CD8αβ lentiviral particles ...
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bioRxiv - Immunology 2022Quote: ... 2 mM EDTA in PBS) and CD31+ and CD45+ cells were sequentially positively selected using anti-CD31 and anti-CD45 MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with APC-eFluor780-conjugated anti-c-Kit (clone: 2B8) and fractionated using anti-APC magnetic beads and LS columns (both Miltenyi Biotec). c-Kit+ cells were then stained with combinations of antibodies as described in Supplemental Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were enriched by MACS using an in-house biotinylated anti-EGFR antibody (Cetuximab, Eli Lilly and Company, Indianapolis, IN, USA) and anti-Biotin Microbeads (Miltenyi Biotec). Purity of enriched CAR modified T cells was analyzed as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... human T-ALL cells were purified after labeling with anti-huCD45 PE conjugated antibody followed by immuno magnetic selection with anti-Phycoerythrin (PE) microbeads (130-048-801, Miltenyi Biotec).
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bioRxiv - Bioengineering 2023Quote: ... the cells were labeled with a rat anti-CPM antibody (in-house) and microbead-conjugated anti-rat immunoglobulin kappa light chain antibody (130-047-401, Miltenyi Biotec). This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec, Macquarie Park, Australia) at 1µg mL-1 on day 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Cancer Biology 2020Quote: Tumors were dissected and minced following the manufacturer’s protocol using MACS C tubes and the mouse Tumor Dissociation Kit (Miltenyi Biotec). After tumor dissociation ...
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bioRxiv - Immunology 2019Quote: ... Murine B cells were enriched by negative selection of IgMpos cells with a mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
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bioRxiv - Molecular Biology 2019Quote: ... Lineage negative cells were collected by applying the Direct Lineage Cell Depletion Kit mouse (130-090-858, Miltenyi Biotec GmbH) and magnetic separation following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
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bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were incubated on ice for 20 minutes with B220 microbeads (Miltenyi Biotec,; 20 μL/1000 μL SM per mouse), washed with SM and enriched on an AutoMACS Pro ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... The cells were resuspended in 200 μl of PBS containing 0.5% BSA and 2 mM EDTA and incubated with 15 μl of mouse FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Immunology 2024Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 3h ...
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bioRxiv - Developmental Biology 2021Quote: ... and then stained with anti-SOX2 (APC; Miltenyi Biotec), anti-OCT3/4 (PE ...
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bioRxiv - Immunology 2021Quote: ... and APC anti-IL-1β (clone REA1172, Miltenyi Biotec) antibody at 1/50 for 30 min at room temperature ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... anti-CD142-PE-Vio616 (130-115-720, Miltenyi Biotec). Anti-GP2 (D277-3 ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... anti-H-2KK-APC (130-117-324, Miltenyi Biotec), Anti-CD177-APC (130-101-512 ...
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bioRxiv - Immunology 2022Quote: ... followed by incubating with anti-APC microbeads (Miltenyi Biotec) for an additional 20 minutes at 4°C.
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bioRxiv - Microbiology 2022Quote: ... FITC-conjugated anti-HLA-DR (clone AC122, Miltenyi Biotec), mouse anti-HSP90 (clone F-8 ...
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bioRxiv - Immunology 2019Quote: ... an anti-XCR1 (PE, REA707 1/200 Miltenyi Biotec) was used to confirm the identity of cDC1 ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by incubating with anti-Biotin microbeads (Miltenyi Biotec) at 4°C for 10 min ...