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Citations for Miltenyi Biotec :
701 - 750 of 1072 citations for Recombinant Human CD40 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody staining for FACS was performed in Flow Cytometry Staining Buffer (ThermoFischer Scientific) and following antibodies were used: PE-GLAST (ACSA-1, Miltenyi Biotec, 1/40), APC-CD133 (13A4 ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained for 10 minutes at room temperature with an intracellular stain cocktail containing the following antibodies: IFN-γ APC and TNF-α PE (all from Miltenyi Biotec). Cells were analyzed on a MACSQuant10 flow cytometer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ml of Enzyme R and 25 ml of Enzyme A (Miltenyi Biotec, Tumor Dissociation Kit 130-095-929). Tumor samples were dissociated using the gentleMACS h_tumor_01 program for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Human ESCs were maintained in StemMACS™ iPS-Brew XF (iPS-B, Miltenyi Biotec) on Laminin-521 (L521 ...
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bioRxiv - Cancer Biology 2020Quote: ... Human MSCs were cultured in StemMACS MSC Expansion Medium (Miltenyi Biotec, Bergisch Gladbach, Germany). Murine MSCs and LPR-MSCs were isolated and cultured as previously described (Albarenque et al. ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CD11b MicroBeads (mouse/human; Miltenyi Biotec Inc., Auburn, CA) and passed through MACS® LS magnetic columns for positive selection of CD11b+ cells ...
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bioRxiv - Immunology 2019Quote: ... filtered human AB serum then subjected to gentle mechanical dissociation using gentleMACS (Miltenyi Biotec) followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were incubated with anti-human CD14 antibodies coupled to magnetic beads (Miltenyi Biotec) and subjected to magnetic separation using LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Human and mouse lung tissues were digested using the Tumor Dissociation Kit (Miltenyi Biotec) for 30 minutes at 37°C and 1,500 rpm ...
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bioRxiv - Immunology 2020Quote: ... total PBMC were enriched in CD14+ monocytes using the human CD14 microbeads (Miltenyi Biotec) for positive magnetic selection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were isolated using the human Pan T cell Isolation Kit (Miltenyi Biotec) and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec, 130-120-709) for 1 h at room temperature in the dark in 0.75% saponin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... human cells were incubated with anti-biotin microbeads (Miltenyi Biotec, Cat#130-090-485) in MACS buffer at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... All setups were stained with 5μL of human FcR blocking reagent (Miltenyi Biotec, USA) to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Human bone marrow CD45+ cells were sorted by immunomagnetic selection with AutoMACS (Miltenyi Biotec) after immunostaining with the CD45 MicroBead Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... and CD38 (20 µl per 107 cells, from human CD38 MicroBead kit, Miltenyi Biotec) followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells ...
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bioRxiv - Microbiology 2022Quote: ... CD8 T cells were depleted using a kit from Miltenyi Biotec (CD8 MicroBeads, human) and cells cultured in RPMI-1640 (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Enzymes and reagents from the human tumor dissociation kit (Miltenyi Biotec: 130-095-929) were added to the tumor tissue along with 1.75 mL of DMEM ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Thawed and fresh samples were dissociated using the Tumor Dissociation Kit (human, Miltenyi Biotec) and tough dissociation protocol on gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... and CD45 (clone 30-F11) (ebioscience), Sca1+ cells were isolated with anti-Sca1-PE-Cy7 (D7, ebioscience) and anti-Cy7 MicroBeads (Miltenyi Biotec, 130-091-652).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... fluorophore-conjugated anti-HA antibody (either anti-HA-APC, catalogue number 130-098-404, or anti-HA-PE, catalogue number 130-092-257, both Miltenyi Biotec, Bergisch Gladbach, Germany) was added to obtain an 11X dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained for 30 min at 4 °C in the dark in PBS using the following antibodies and conjugates: qNSCs and aNSCs were discriminated by a combination of PE-conjugated anti-mGLAST (1:50, clone ACSA-1, Miltenyi Biotec, Bergisch Gladbach, Germany), APC-conjugated anti-mCD133 (1:75 ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... the labeled cells were incubated with 100 µl per 10 million cells of MACS dead cell removal beads (Miltenyi Biotec, 130-090-101) and 20 µl of each microbeads specific against AlexaFluor647 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension of the lung was generated from influenza infected mice as described above and labeled and enriched with CD11c and CD11b or CD8 microbeads (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2020Quote: ... and spike proteins (Prot_S) were purchased from Miltenyi Biotec. Optimal peptides for the manufacture of HLA class I tetramers were synthesized at >95% purity by Peptides & Elephants GmbH ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Structures were enzymatically dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec) with a gentleMACS™ Octo Dissociator (with heaters ...
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bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
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bioRxiv - Cell Biology 2021Quote: Human neutrophils were isolated on a MACS LS column system (Miltenyi Biotec #130-042-401), enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601 ...
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bioRxiv - Immunology 2022Quote: ... resting NK cells were obtained using the human NK cell isolation kit (MACS Miltenyi Biotec), following manufacture’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... Human monocytes were isolated from PBMC using a magnetic-based monocyte isolation kit (Miltenyi Biotec) and differentiated into macrophages with GMCSF (50 ng/mL ...
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bioRxiv - Systems Biology 2020Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (MACS Miltenyi Biotec) and LS Columns (Miltenybiotec) ...