Labshake search
Citations for Miltenyi Biotec :
651 - 700 of 1072 citations for Recombinant Human CD40 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
-
bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures with the surface antibody CD34-PE (clone AC136, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
-
bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
-
bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
-
bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
-
bioRxiv - Genetics 2020Quote: ... depletion of lineage+ cells from MNCs was performed using Biotin labeled Lin+ cocktail and Streptavidin MicroBeads (Macs; Miltenyi Bio-tec) and magnetic columns (Macs; Miltenyi Biotec). For cell sorting of LSK ...
-
bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...
-
bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
-
bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
-
bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
-
Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
-
bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
-
bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
-
Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... Human lung single T cells were prepared from surgical resection and lungs in patients as previously described by selection with biotin-labeled antibodies by autoMACs (Miltenyi Biotec) (Yuan et al. ...
-
bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
-
bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
-
bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
-
bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells from BM of non-metastasized breast cancer patients were subjected to immunofluorescent staining for EpCAM (Ber-EP4-FITC, Agilent or HEA-125-PE, Miltenyi Biotec, Germany). Positive cells were isolated with a micromanipulator (Eppendorf PatchMan NP2 ...
-
bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with anti-human CD8-FITC (BW135/80, Miltenyi biotec) and anti-human CD4-PE-Cy7 (SK3 ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 1X human FcR blocker (stock: 50x Mac’s Miltenyi Biotec 130-059-901). After washing ...
-
bioRxiv - Immunology 2021Quote: Human and mouse DCs were stained with the following antibodies from Miltenyi Biotec or as specified ...
-
bioRxiv - Microbiology 2020Quote: ... and monocytes were isolated using human CD14 MicroBeads (130-050-201, Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
-
bioRxiv - Immunology 2020Quote: ... Two-fold dilutions of the anti-human CD303 BDCA2 antibody (AC144) (Miltenyi Biotec), chimeric CBS004 (Capella Bioscience ...
-
bioRxiv - Neuroscience 2022Quote: ... human BDNF (50 µL, 100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (25 µL ...
-
bioRxiv - Cancer Biology 2019Quote: ... an unconjugated mouse anti-human CD133 antibody was used (Miltenyi Biotec; 1:20), and for experiments including FAP ...
-
bioRxiv - Cell Biology 2021Quote: ... Human NP tissue samples were transported in MACS Tissue Storage Solution (Miltenyi Biotec) immediately after surgical resection ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples for surface staining were incubated with human FcR Blocking Reagent (Miltenyi Biotec) together with PE-conjugated mouse anti-GLAST 1:15 (Miltenyi Biotec 130-095-821 ...
-
bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec) and activated with T Cell TransAct (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2021Quote: ... Human CD14 microbeads were used in combination with LS columns (both Miltenyi Biotec) to positively select CD14+ blood monocytes ...
-
bioRxiv - Immunology 2021Quote: ... T cells were selected using human CD4 microbeads and MS columns (Miltenyi Biotec). Procedure was carried out according to manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Monocytes were isolated from PBMCs by anti-human CD14 (REA599, APC, Miltenyi Biotec) antibody labeling and subsequent isolation using a magnetic anti-APC-Microbead Kit (Miltenyi Biotec) ...
-
bioRxiv - Pathology 2023Quote: ... followed by immunomagnetic separation using a human anti-CD14 purification kit (Miltenyi Biotec). The cell purity was always confirmed by flow cytometry and was superior to 95% ...
-
bioRxiv - Biophysics 2023Quote: ... Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labelled with the human NK Cell Isolation Kit (Miltenyi Biotec), with subsequent NK cell isolation using the “depletes” program on an autoMACS Pro Separator and collecting the negative fraction ...
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were activated with TransAct Human T cell activation reagent (Miltenyi Biotec) and 100 U/ml human IL-2 IS (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 50 IU/ml premium-grade human IL2 (Miltenyi Biotec, 130-097-746) at 1x106 cells per mL and cm2 ...