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Citations for Miltenyi Biotec :
701 - 750 of 996 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2021Quote: ... also known as LPEC-1 and -6 in previous studies) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) 10-13 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
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bioRxiv - Immunology 2021Quote: B cells were purified from splenocytes by positive selection using magnetic beads-conjugated monoclonal anti-mouse B220 antibody (clone: RA3-6B2, Miltenyi Biotec, Bergisch Gladbach, Germany). Isolated B cells (5 × 105/mL ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... CD14 + monocytes were then isolated from the PBMCs by positive selection using magnetic beads conjugated to anti-CD14 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Cancer Biology 2023Quote: CD40 stimulation medium were prepared by incubation of 40 uL of CD40 ligand and 40 uL of cross-linking antibody (Miltenyi Biotec 130-106-196) mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196) ...
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bioRxiv - Cancer Biology 2022Quote: ... and efficiently depleted of non-target cells by addition of magnetic microbeads conjugated to mouse anti-biotin monoclonal IgG1 antibodies and passage through LS ferromagnetic columns (Miltenyi Biotec, 130-042-401). The flow through ...
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bioRxiv - Physiology 2023Quote: ... the cells were mixed with CD45 antibody-coated magnetic beads (CD45 MicroBeads, 130-052-301) and then passed through a MACS LD column (Miltenyi Biotec, 130-042-901) on a MidiMACSTM separator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
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bioRxiv - Neuroscience 2024Quote: ... The monocyte population was enriched by positive selection of CD14-labeled target cells using a human magnetic antibody cell sorting (MACS) system (Miltenyi Biotec, Bergisch-Gladbach, Germany), according to the manufactureŕs procedure ...
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bioRxiv - Cell Biology 2024Quote: ... Cells then underwent magnetic activated cell sorting to isolate podocytes (using biotin anti-podoplanin antibody, BioLegend, 337015, conjugated to anti-biotin microbeads, Miltenyi Biotec, 130-090-485) and glomerular endothelial cells (gEC ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... GLAST (1:500, mouse monoclonal; Miltenyi Biotec cat. no. 130-095-822), or NFIA (1:200 rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec, #130-092-613, 1:100), Anti-Biotin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... in mTeSR™1 medium supplemented with StemMACS™ Y27632 (Miltenyi Biotec). Once the cells reached greater than 95% confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... and CD338-PE (ABCG2-PE, Miltenyi Biotec, 130-105-010, 1:40) followed by 1hour incubation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... at a 1:100 dilution and human FcR blocking reagent (Miltenyi Biotec) at a 1:20 dilution on ice for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS supplemented with 1% Fc Block (MACS, Miltenyi Biotec) and 0.5% BSA for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... anti-human IgA (Miltenyi Biotec, Clone IS11-8E10, APC, 1:100, 4℃); anti-human IgM (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... 25 ng mL−1 macrophage–colony stimulating factor (w/v; Miltenyi Biotec), and 100U mL−1 penicillin/streptomycin (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 supplement and 2% NeuroBrew-21 (Miltenyi Biotec, 130-093-566), as well as 10 ng/ml of human bFGF (Miltenyi Biotec 130-093-838 ...
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bioRxiv - Immunology 2020Quote: Peritoneal B1a cells were obtained through negative magnetic-activated cell separation with a cocktail of antibodies depleting other than B1a cells achieving more than 90% purity in isolation (Miltenyi Biotec B.V., Utrecht, The Netherlands). B1a cells were subsequently counted and plated in IMDM medium (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were positively selected using anti-CD4 and CD8 antibodies through the Prodigy TCT program on a CliniMACS Prodigy (Miltenyi Biotec, Cat# 200-075-301). After selection ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were then magnetically sorted to enrich CD14+ cells using microbeads conjugated to a monoclonal human CD14 antibody (Miltenyi Biotec; Cat No. 130-050-201). Cryopreserved Beagle canine CD14+ ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec). Primary cell cultures were maintained in the presence of mouse embryonic fibroblasts (STO ...
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bioRxiv - Cell Biology 2020Quote: Astrocytes were purified using the Anti-GLAST (ACSA-1) MicroBead Kit (Miltenyi biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry was done using anti-CD133/1-PE (Miltenyi Biotec, clone AC133) and anti-GD2-APC (Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-cardiac troponin-T-APC (Miltenyi Biotec, 130-120-403, 1:50) in blocking buffer (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Anti-foam Y-30) in M-tubes (gentleMACS™ Tubes, Miltenyi Biotec). Organs were then mechanically homogenized using a gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... PE anti-human MSCA1 (Clone W8B2, dilution 1/10, Miltenyi Biotec, Paris, France), APC antihuman CD271 (Clone ME20.4-1.H4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC-conjugated anti-ACSA-2 (Miltenyi Biotec, 130-116-245, 1:100) antibodies for 30 min at 4℃ ...
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bioRxiv - Microbiology 2024Quote: ... APC-coupled mouse anti-Human CD83 (Miltenyi Biotec, 130-094-186; 1/50), BV510-coupled mouse anti-Human CD83 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed again and blocked using 1% Fc block (Miltenyi Biotec) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/ml PepTivator SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec), or an equivalent volume of DMSO (or sterile water as indicated ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-mouse CD45-PE (1:50 dilution; 130-110-797, Miltenyi Biotec) for 10 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... diluted at 1:16 in MACS buffer (130-091-221, Miltenyi Biotec, USA) at 4°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... washed with 1x PBS and were treated with 1% Fc block (Miltenyi Biotec) in 1x PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were ventralized by adding 1 μM purmorphamine (Miltenyi Biotec, 130-104-465). 10 ng/ml brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were then purified using MACS with anti-TRA-1-60 MicroBeads (Miltenyi Biotec) on LS columns ...
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bioRxiv - Bioengineering 2021Quote: ... 1:20 in autoMACS Rinsing Solution (130-091-222; Miltenyi Biotec B.V. & Co. KG). For degassing ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with FcR Blocking Reagent (Miltenyi Biotec, Cat no. 130-059-901, 1:50) and subsequently stained at 4°C with antibodies against following human antigens ...
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bioRxiv - Neuroscience 2021Quote: ... μMACS microcolumns were washed 1× with 100 μL nucleic acid equilibration buffer (Miltenyi Biotec), followed by 5× washes with 100 μL 1× wash buffer ...