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Citations for Miltenyi Biotec :
551 - 600 of 996 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Developmental Biology 2022Quote: ... then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8, eBiosciences) and streptavidin-conjugated magnetic beads (Miltenyi Biotec). This generates a >95% pure preparation of e16.5 bipotent alveolar progenitors (Sftpc+ Rage+ cells) ...
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bioRxiv - Genomics 2020Quote: ... and CD138+ multiple myeloma cells isolated from bone marrow biopsies by positive selection on antibody-conjugated magnetic beads (Miltenyi Biotec), were obtained with I.R.B ...
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bioRxiv - Neuroscience 2021Quote: ... kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia) according to the manufacturer’s protocol (Miltenyi Biotec) and as previously described27 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... and Ter119 APC- or PE-labeled antibodies followed by magnetic depletion with anti-APC or -PE Micro-Beads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were depleted using Igκ/CD4/CD8/GR1/Ter119/TCRb/TCRgd/CD11b/CD11c/NK1.1 antibodies and anti-PE Micro-Beads (Miltenyi Biotec), and WT (CD45.1+CD45.2− ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in 180 μl artificial seawater containing 0.05% BSA and we added 20 μl of CD4 antibody (Miltenyi Biotec) conjugated with magnetic beads ...
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Plant Biology 2022Quote: ... Solubilized proteins from microsomal fraction were immunoprecipitated using super-paramagnetic micro MACS beads coupled to monoclonal anti-FLAG antibody according to the manufactureŕs instructions (Miltenyi Biotec). WT Col-0 extract was used as a control of the unspecific binding of endogen PIN2 ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T were purified using magnetic beads coated with anti-CD4 antibody (clone L3T4) according to the manufacturer’s instructions (Miltenyi Biotec). 5×105 2D2 CD4+ T cells were injected i.v ...
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bioRxiv - Immunology 2023Quote: ... and incubated with a biotinylated Ter-119 antibody (Table S1) and remaining erythrocytes were depleted with anti-biotin microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Afterward ...
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bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then stained with fluorochrome-conjugated antibodies (anti-mouse-BV421-CD45) and anti-mouse-PE-O4 (Miltenyi Biotec)) for 40 min at 4°C to characterize oligodendrocytes (CD45-O4+) ...
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bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation was carried out using micromagnetic bead-coupled GFP antibodies from µMACS GFP Isolation Kit (Miltenyi Biotec 130-091-125) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Cells underwent a TCRαβ depletion step (anti-TCRαβ antibody REA652 clone, anti-biotin microbeads and LD column, all Miltenyi Biotec) with an overnight recovery followed by cryopreservation in 45% TexMACS media (Miltenyi Biotec) ...
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bioRxiv - Physiology 2024Quote: ... Cells were filtered through 35 μm filters and incubated with CD31 magnetic conjugated bead antibodies (#130-097-418, Miltenyi Biotec). Positive selection for CD31+ cells was performed using magnetic separator columns (#130-042-201 ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in 400 µl DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). Small samples of cells were fixed and air-dried on glass slides to control for cell purity by immunocytochemical staining of GLUL ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). After 24h or 48h ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:10 CD61-PE-Vio770 (Miltenyi Biotec, 130102627) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... CD11b (Clone M1/70, PE, 1:50, Miltenyi Biotec), Ly6G (clone 1A8 ...
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bioRxiv - Systems Biology 2020Quote: ... 1:10 Emb-PE (Miltenyi Biotec, 130-107-948). The staining was done in the dark at 4°C with agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% v/v BSA (Miltenyi Biotec, 130-091-376), 0.5% v/v Triton-X (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng/ml TGFβ (130-095-067, Miltenyi Biotec), 12.5 ng/ml IFN-γ (130-096-872 ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...
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bioRxiv - Cell Biology 2021Quote: ... and MEFSK4 (1:50 Miltenyi Biotec 130-120-802). This allows for the removal of CD11b cells which are known to express high levels of MBNL1 ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated with TransAct (1:150, Miltenyi Biotec) or PBS (negative activation control ...
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bioRxiv - Developmental Biology 2023Quote: ... 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec), 1× MEM NEAA (GIBCO™) ...
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bioRxiv - Immunology 2024Quote: ... MEFSK4-PE (clone mEF-SK4, 1:100, Miltenyi Biotec), CD64-PE/Cy7 (clone X54-5/7.1 ...
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bioRxiv - Systems Biology 2024Quote: ... 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec), 1× MEM NEAA (GIBCO™) ...
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bioRxiv - Molecular Biology 2024Quote: ... and CD34 (1/100 CD34-PE-Vio770; Miltenyi Biotec). Cells were analyzed by flow cytometry using the Novocyte analyzer (Agilent ...
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bioRxiv - Bioengineering 2024Quote: ... with 1% FcR blocking reagent (Miltenyi Biotec, Gaithersburg, MD) on ice ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... (Supp Fig.1) (Miltenyi Biotec, Inc., San Diego, CA) and were stimulated with anti-CD2/CD3/CD28 monoclonal Ab beads (Dynabeads ...
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bioRxiv - Cell Biology 2024Quote: ... CD31-APC (1:50) (130-111-354, Miltenyi Biotec), CD34-PE (1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: Murine liver primary ECs were isolated from athymic nude mice also using MACS microbead-conjugated antibodies for murine CD31 (Miltenyi Biotec) and cultured in MV2 EC culture medium as described above ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...