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Citations for Miltenyi Biotec :
701 - 750 of 909 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2) 4.17 mM GadoSpin-P (n=6; MW=200 kDa; Miltenyi Biotec, Bergisch Gladbach, Germany); and 3 ...
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bioRxiv - Neuroscience 2023Quote: ... pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024, Miltenyi Biotec) supplemented with 0.2 U/μL RNase inhibitor (#2489605 ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Immunology 2024Quote: ... was realized using the SARS-CoV-2 RBD B-cell MicroBead kit (Miltenyi Biotec, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Lymph nodes were stained for CD19 expression using the panel described in supplemental table 1 following incubation with FcR blocking reagent (Miltenyi Biotec # 130-092-575). Cells were analyzed on a BD LSRFortessa ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were cultured for one week and then the same procedure was followed with anti-Prominin-1 MicroBeads (Miltenyi Biotec, Cat# 130-092-564) for the negative selection of radial glia ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was replaced with a neural maintenance medium (NMM) supplemented with 1 µM StemMACS™ LDN-193189 (Miltenyi Biotec, #130-106-540) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Plant Biology 2022Quote: ... isolated from transfected protoplasts were separated by SDS-PAGE and subjected to immunoblotting using an anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000).
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were cut into smaller pieces and transferred into Enzyme Mix 1 (955 μl of Buffer Z, 25 μl of Enzyme P [Miltenyi Biotec, 130-094-802]) in a 1.5-ml tube ...
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bioRxiv - Immunology 2023Quote: ... cells were transduced with 2uL per well of concentrated Lenti-CRISPR-v2-GFP supernatant (transduction efficiency kept <20% to promote single copy integration) and 1uL Vectofusin-1 (1X, Miltenyi Biotec, 130-111-163) diluted in 50 uL of cord-blood optimized media ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellet was gently tapped to loosen it and then resuspended in 1 mL of wash buffer containing 50 μL of CD43 (Ly-48) MACS microbeads (Miltenyi Biotec, #130-049- 801). The mixture was incubated on ice for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... to label and exclude islet cells, HPx1-Dylight 488 (Novus, NBP1-18951G) to label acinar cells and CD133/1(AC141)-PE (Miltenyi Biotec 130-080-801), CD133/2(293C3)-PE (Miltenyi Biotec 130-090-853 ...
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bioRxiv - Developmental Biology 2024Quote: ... was added to the bottom of the plate and cultured in the organoid culture medium 1 (50% DMEM/F12, 50% MACS Neuro Medium (Miltenyi Biotec, 130-093-570), 1 mM MEM non-essential amino acids ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Immunology 2024Quote: ... IMhu-M cells were cultivated in IL-4/GM-CSF MoDC differentiation medium (Miltenyi Biotec, Cat. No. 130-094-812), when indicated ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and 4% BSA in HBSS) was added per tissue and tissues were further homogenized with the GentleMACS dissociator (Miltenyi Biotec) using the following program ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 minutes at 4°C with rotation and passed through an LS separation column (Miltenyi Biotec, 130-042-401) inserted into a Quadro MACS Multi Stand magnetic stand (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: Astrocyte and microglia were purified by anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 40 ng/ml basic fibroblast growth factor-2 (FGF2) (Miltenyi Biotec, Bergisch Gladbach, Germany), 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and astrocytes were respectively isolated using Anti-CD11b and Anti-ACSA-2 MicroBeads (Miltenyi Biotec). Cells were counted and resuspended in 10-15 µL and 20-30 µL of buffer (0.05% BSA in DPBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the media was further supplemented with 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 EAE late stage samples were processed debris removal solution (Miltenyi Biotec, 130-107-677) according to the manufacturer’s protocol to enrich more OPCs ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated in dissociation solution (Multi Tissue Dissociation Kit 2; Miltenyi Biotec; Catalog # 130-110-203) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... in a six-well plate in 2 ml StemMACS iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, Germany). Medium was supplemented with 100 U/ml penicilin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The hiPSC-CMs were incubated with the conjugated antibodies cTnT-Vioblue or cTnT-FITC (1:50, Miltenyi Biotec, #130-120-402 or #130-119-575). All antibodies were diluted in permeabilization medium (medium B ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2020Quote: ... DC differentiation was carried out over 6 days with 250 U/ml IL-4 and 50ng/ml GM-CSF (Miltenyi Biotec).
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... The adherent monocytes were then washed twice with PBS and resuspended in RPMI supplemented with the cytokines GM-CSF (1000 U/mL) and IL-4 (500 U/mL) (Miltenyi Biotec). Differentiation to immature MDDCs was achieved by incubation at 37 °C with 5% CO2 for 7 days and subsequent activation with cytokines addition every other day ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...