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Citations for Miltenyi Biotec :
651 - 700 of 909 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Immunology 2021Quote: ... then positively selected using anti-ACSA-2 MicroBeads (Miltenyi Biotec, Cat# 130-097-678). Total RNA was prepared using TRI Reagent (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte ACSA-2+ cell sorting was performed using an autoMACS Pro Separator (Miltenyi Biotec). After isolation ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-photon imaging was also performed with a TrimScope Matrix II from Miltenyi Biotec using an Olympus 25X W/NA 1.05 objective at a wavelength of 850 nm owing to Spectra Physics Insight X3+ laser.
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bioRxiv - Neuroscience 2023Quote: ... followed by ACSA-2 MicroBeads (cat # 130-097-678, MACS Miltenyi Biotec, Auburn, CA) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
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bioRxiv - Genetics 2024Quote: ... iPSCs were mechanically passaged in the presence of 2 μM Thiazovivin (Miltenyi Biotec, UK).
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 Prot_S 130127953) (0.6 nmol/mL each) (Miltenyi Biotec; Bergisch Gladbach, Germany). After 6 days of incubation at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed to dopaminergic patterning medium composed of CTS™ DMEM-F12 (with CTS™ N2 1:100) plus freshly added 200 ng/ml GMP Sonic Hedgehog (SHH, 130-095-727, Miltenyi Biotec), 100 ng/ml GMP FGF-8b (130-095-740 ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly cells were strained through a 70 μm cell strainer and then stained with CD11b (1:50 Miltenyi Biotec 130-113-800) and MEFSK4 (1:50 Miltenyi Biotec 130-120-802) ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 250 μl of each sample were incubated on ice for 1 h with 50 μl of α-GFP µMACS beads (Miltenyi Biotec). Immunoprecipitates were isolated using the μMACS GFP isolation kit ...
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bioRxiv - Microbiology 2022Quote: ... Tonsil and lung tissue samples were dissected to approximately 1 g pieces and placed into gentleMACS C Tubes (Miltenyi Biotec, Auburn, CA). The weight of each sample was recorded and PBS was added to a 10% weight/volume ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of each sample was transferred to an Eppendorf tube and assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). GFP-labeled B ...
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bioRxiv - Cell Biology 2024Quote: ... an equivalent of 1,000 human CD34+ cells from mBM and hOss were plated in 1 mL of methylcellulose-based medium (130-125-042, StemMACS™ HSC-CFU Assay Kit; Miltenyi Biotec) that contained human SCF ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor portions were minced to 3-4 mm pieces and dissociated using a Tumor Dissociation Kit (Miltenyi Biotec), followed by the clearance of red blood cells with RBC lysis buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Human myeloid CD1c+ DC were isolated from blood and kidney by positive immuno-magnetic selection using anti-CD1c/BDCA-1 microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) or on a FACS ARIA (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Immunology 2024Quote: The left lung was weighed and homogenized in 1 mL of RPMI supplemented with 10% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of microdissected vmPFC grey matter was homogenized in 2 ml of cold homogenization buffer (1M sucrose + 1% BSA dissolved in DEPC-treated water) using the benchtop gentleMACS™ Dissociator (Miltenyi Biotec, Germany) with the rotating paddle set to the Lung 02.01 program ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...
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bioRxiv - Cell Biology 2024Quote: Each 60 mg portion of freshly obtained skeletal muscle was suspended in 1 mL ice-cold Lysis Buffer (Mitochondria Isolation Kit, human, 130-094-532, Miltenyi Biotec, CA, USA) containing 5 μL/mL Protease Inhibitor Cocktail (P8340 ...
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bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
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bioRxiv - Microbiology 2020Quote: ... and the medium on the monocytes was replenished with X-vivo 15 + L-glutamine containing interleukin-4 (Miltenyi Biotec) and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded 2 or 3 days before transfection experiment by MACSfectin reagent (Miltenyi Biotec), to achieve cell density recommended by the supplier.
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bioRxiv - Neuroscience 2022Quote: ... Astrocyte fraction was enriched using Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec, #130-097-679).
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bioRxiv - Immunology 2020Quote: ... anti-Dectin-2 PE (REA1001) and anti-CD64 APC (REA286) were purchased from Miltenyi Biotec; rat anti-Dectin-2 (D2.11E4 ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated for activation for 3 days supplemented with 20ng/μL IL-2 (Miltenyi Biotec) and CD3/CD28 Dynabeads Human T-Activator (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... followed by addition of 150 μL Anti-ACSA-2 microbeads (Miltenyi Biotec 130-097-678). The sample was placed on a rotating wheel at 4°C for 30 min ...
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bioRxiv - Immunology 2024Quote: ... CD8- thymocytes were obtained by negative selection using anti-CD8 (Ly-2) MicroBeads (Miltenyi Biotec). The cells were then stained with fluorescently labeled antibodies to CD4 ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with either PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec) or S recombinant protein (Sino Biological ...
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bioRxiv - Immunology 2024Quote: Tumors were dissociated using Multi Tissue Dissociation Kit 2 (Miltenyi Biotec, Cat#: 130-110-203) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the membrane glycoprotein (PepTivator® SARS-CoV-2 Prot_M) were obtained from Miltenyi Biotec. The PepTivator® Peptide Pools are constituted by peptides of 15 amino acid length with 11 amino acid overlap ...