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Citations for Miltenyi Biotec :
651 - 700 of 750 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Surface protein profiling of primary and TERT EVs was performed via Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV IO Kit, human, 130-108-813, Miltenyi Biotec). EV-containing samples were processed as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Bone marrow aspirates from healthy donor and pathological patients were enriched for plasma cells by magnetic cell separation using a human CD138 positive selection and Macs Separator kits (Miltenyi Biotec, Germany).
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... B cells were isolated using either the StraightFrom™ Buffy Coat CD19 Microbead Kit or the Human B cell Isolation Kit II (both from Miltenyi Biotec), following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected for TSDR demethylation analysis and stored at −80 as cell pellets or expanded with 500-1000 U/mL human rIL2 (Miltenyi Biotec/Chiron) +/− 100 nM rapamycin (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T cells were magnetically separated by negative selection with a Human Pan T cell isolation Kit (#130-096-535, Miltenyi Biotec, Germany) and stored long-term in Bambanker serum-free cell freezing medium (#BB01 ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells from cord blood or adult donors were sorted using microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (Miltenyi Biotec MACS) through positive magnetic bead selection on LS columns.
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bioRxiv - Microbiology 2024Quote: ... and CD34+ hematopoietic stem cells (HSCs) were isolated from these mononuclear cells using CD34 microbead kit (Miltenyi Biotec CD34 MicroBead Kit, Human). The purity of isolated CD34+ cells were accessed by flow cytometry using mouse anti human CD34 (BD Bioscience).
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bioRxiv - Biochemistry 2021Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL, Melbourne, Australia) followed by CD14+ MACS separation (Miltenyi Biotec, Sydney, Australia). The isolated monocytes were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD14+ human monocytes were isolated from fresh peripheral blood mononuclear cells (PBMCs) using CD14 MACS MicroBeads (Miltenyi Biotec, Cat. No. 130-050-201) and cultured in MoDC differentiation medium at a density of 106 cells/ml for 6 days ...
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bioRxiv - Neuroscience 2023Quote: Human tumor tissue samples were acquired during neurosurgical biopsy or open resection and stored in Tissue Storage Solution (Miltenyi Biotec, 130-100-008) for 2-30 hours until further processing for scRadiotracing ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Mouse cells were depleted from the xenograft tumor to enrich for human cells using Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694) as previously described24 ...
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bioRxiv - Cancer Biology 2024Quote: Primary human monocytes were isolated from PBMCs from three donors using a negative selection classical monocyte isolation kit (Miltenyi Biotec 130-117-337) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: CD34+ cells were purified from human cord blood (Cord Blood Bank of Beijing) using the MACS MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The two-phase erythroid differentiation protocol was modified from the previous study23.
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bioRxiv - Immunology 2024Quote: ... Human primary NK cells were isolated from the cryopreserved PBMCs utilizing the NK Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-092-657) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Primary T cells were sorted from primary PBMC from healthy donors using Pan T cell isolation kit – human (Miltenyi Biotec, # 130-096-535). Isolated T cells were stimulated with plate-bound anti-CD3 and anti-CD28 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: Each 60 mg portion of freshly obtained skeletal muscle was suspended in 1 mL ice-cold Lysis Buffer (Mitochondria Isolation Kit, human, 130-094-532, Miltenyi Biotec, CA, USA) containing 5 μL/mL Protease Inhibitor Cocktail (P8340 ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Cancer Biology 2021Quote: ... MM cells were pelleted and resuspended in flow cytometry buffer (1× PBS with 1% FBS) then incubated with human FcR blocking reagent (Miltenyi Biotec, # 130-059-901) before staining with diverse fluorescent dye-conjugated monoclonal antibodies ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2024Quote: ... Tregs were isolated from fresh PBMCs by magnetic separation using the CD4+CD25+CD127dim/- human Treg isolation kit (Miltenyi Biotec, Cat: 130-094-775). Tregs were then used directly for analysis or assays.
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bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...