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Citations for Miltenyi Biotec :
601 - 650 of 750 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... prior to magnetic bead separation in order to obtain a minimum of 90% CD34+-enriched cell population (human Diamond CD34 isolation kit, Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2021Quote: Immunoaffinity isolation of uEVs was performed using a commercially available kit (Exosome Isolation Kit Pan, human, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25- cells were then obtained by depletion of CD25+ T cells using CD25 positive selection (CD25 microbeads II, Human, Miltenyi Biotec) and collecting the unlabeled cells.
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of CD81- (for all samples) or CD63- (for MS and HC samples) isolation beads (Exosome Isolation Kit, human Miltenyi Biotec) were added to 2 ml of diluted sample ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec) at an MOI of 1:100 (72 ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: CD4+ and CD8+ T cells were then isolated from PBMCs by magnetic associated cell sorting (MACS) using the CD4+ or CD8+ human T cell isolation kit (130-096-533 or 130-096-495; Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were isolated from PBMCs by negative selection using the human NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). NK cells were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec; CAT#130-097-095) was used to purify T cells ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched using human anti-CD8 MicroBeads and the automated magnetic activated cell sorting (autoMACS) system (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Genetics 2024Quote: ... were harvested and T cells were isolated by magnetic separation with the human Pan T Cell isolation kit and stimulated using TransAct (both Miltenyi Biotec). Cells were then cultured in TexMacs medium (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... CB mononucleated cells were enriched for CD34+ cells using the human CD34 MicroBeads Kit following manufacturer instructions (130-046-703, Miltenyi Biotec), assessed for purity (≥70% CD34+ cells) ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from healthy donors by Ficoll gradient followed by primary CD4+ T-cell isolation by negative selection and magnetic separation using the human CD4+ T Cell Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... Then the medium was replaced by RPMI supplemented with 10% human serum type AB and 100ng/mL GM-CSF (Miltenyi Biotec). The following day ...
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bioRxiv - Immunology 2024Quote: ... Tissue samples were then minced into fragments and enzymatically digested for 1 hour at 37°C into single-cell suspensions using the gentleMACS Octo dissociator and human tumor dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets of Treg and non-Treg cells were collected and stored at -80°C directly after isolation or after 72h stimulation using human Treg Suppression Inspector beads (130-092-909, Miltenyi Biotec) in X-VIVO 15 (02-060F ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from whole blood by the ficoll density gradient method (CL5020) and CD4+ T cells were selected using the human CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... tumour fragments from the lymphatic melanoma and skin lesion to chest wall metastases were obtained respectively during the surgical procedure and dissociation was performed on the same day utilising the human tumour dissociation kit (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Different sample types were subjected to bead-based multiplex EV analysis by flow cytometry (MACSPlex EV IO Kit, human, Miltenyi Biotec). Unless indicated otherwise ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor fragments were minced and enzymatically dissociated using a Miltenyi Human Tumor Dissociation Kit and Miltenyi gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were subjected to a CD45+ enrichment using anti-CD45 microbeads (mouse: 130-052-301, human: 130-045-801, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Bone marrow myeloma cells were enriched using human CD138 Microbeads and Manual MACS Cell Separation Units (Miltenyi Biotec, Bergisch, Gladbach, Germany). DNA of CD138-positive fractions was isolated using the Allprep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone marrow myeloma cells were enriched using human CD138 Microbeads and Manual MACS Cell Separation Units (Miltenyi Biotec, Bergisch, Gladbach, Germany). DNA of CD138-positive fractions was isolated using the Allprep Kit (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: Surface protein profiling of primary and TERT EVs was performed via Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV IO Kit, human, 130-108-813, Miltenyi Biotec). EV-containing samples were processed as follows ...
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bioRxiv - Bioengineering 2024Quote: Hemolysates were prepared from either erythroid cell after 18 days of differentiation for in vitro differentiation experiments or human erythroid cells directly purified from xenotransplanted mouse BM by CD235a microbeads (130-050-501, Miltenyi Biotec). For single-chain globin variants ...
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bioRxiv - Cancer Biology 2024Quote: Human monocytes were purified from buffy coats of healthy donors as reported elsewhere38 or using Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach ...