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Citations for Miltenyi Biotec :
651 - 700 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue of single mice was digested with trypsin and DNase according to the guidelines of the Neural Tissue Dissociation Kit (T) (Miltenyi Biotec) using a Gentle MACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... the wound tissues were firstly digested by the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) for enzymatic epidermal-dermal separation ...
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bioRxiv - Immunology 2023Quote: ... the wound samples were enzymatically digested by the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) to achieve the epidermal-dermal separation ...
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bioRxiv - Systems Biology 2019Quote: ... and mouse cells expressing CD45 (Miltenyi Biotec, 130-052-301) or TER119 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with mouse FcR Blocking Reagent (Miltenyi Biotec) for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133 ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2023Quote: Tumor samples harvested from mouse mammary glands were dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec,130-096-730), gentleMACS C tubes (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... 14 by mixing cut tumors with Mouse Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2023Quote: ... Tumor tissues were dissociated using mouse/human tumor dissociation kit (Miltenyi Biotec), wherein tumors are chopped into smaller pieces ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified with either anti-CD8 or anti-CD4 magnetic beads using MACS columns (Miltenyi Biotec, Auburn, CA) prior to cell surface staining ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ and CD8+ T cells were isolated by MACS from monocyte-depleted PBMC using CD4 and CD8 microbeads (Miltenyi Biotec). These primary cells were cultured in X-vivo 15 (Lonza ...
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bioRxiv - Immunology 2022Quote: ... PMEL T cells were isolated from the splenocytes of the Thy1.1+ PMEL TCR transgenic mice by CD8a+ negative selection (Miltenyi Biotec). Each irradiated Krt14-Kitl* mouse received 1×106 cells of PMEL plus 1.8×106 cells of the peptide-pulsed MHC-II+ ...
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bioRxiv - Immunology 2020Quote: ... A pool of CD4+ and CD8+ T cells was isolated using CliniMACS CD4 and CD8 GMP MicroBeads (Miltenyi Biotec, Germany). GMP-grade lentiviruses ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested from femur and tibiae of 6–9 week-old CD11c-DTR or CD11c-DTR Rag2−/− SJL mice and depleted of T cells using anti-CD3 and anti-CD8 magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Wild type or Rag2−/− SJL mice were gamma-irradiated with 950 rads from a 60Co source ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were depleted for CD4 and CD8 T cells using magnetic bead depletion techniques according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Cells were rinsed once with media and then CD3+ T-cells were isolated via positive magnetic selection or pan T-cells were isolated via negative selection according to the manufacturer’s protocol (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
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bioRxiv - Cancer Biology 2022Quote: CD3+ T cells from healthy donors were separated from PBMCs using CD3 immunomagnetic beads (#130-097-043, Miltenyi Biotec, Germany), then amplifing using CD3/CD28 stimulation beads (#11131D ...
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bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
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bioRxiv - Immunology 2022Quote: ... the lung draining lymph nodes (DLN) and spleens were harvested and donor T cells were isolated using MACS (Miltenyi Biotec) based on their congenic marker (CD90.1) ...
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T-cells and monocytes were purified from PBMCs by positive selection using CD4 (Miltenyi Biotec; 130-045-101) and CD14 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then enriched for KIT expressing cells by magnetic activation cell sorting (MACS) using CD117 MicroBead Kit (Miltenyi Biotec), following manufactureŕs instructions.
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bioRxiv - Cancer Biology 2023Quote: ... and further processed into dead-cell depleted single cell suspensions following manufacturer’s protocol using Tumor Dissociation Kit and MACS Dead Cell removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... Co-IP assays from transfected protoplasts were performed with C-Myc Isolation Kit (130-091-123) and DYKDDDDK (Flag) Isolation Kit (130-101-591) from Miltenyi Biotec. Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently incubated with anti-CD117/c-kit-APC (1:50; Miltenyi Biotec; RRID: AB_615056). Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences) ...
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bioRxiv - Pathology 2020Quote: ... Dead Cell Removal Kit (Miltenyi Biotec) was used to remove the cells with low viability ...
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bioRxiv - Neuroscience 2023Quote: ... dead cell removal kit (Miltenyi Biotec) to purify single cells ...
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bioRxiv - Cancer Biology 2019Quote: ... the live cell fraction was subjected to a similar MACS based isolation by application of mouse MDSC isolation kit (Miltenyi Biotec, 130-094-538). Isolated cells were washed twice with cold MACS buffer and counted with a hemocytometer and diluted in cold PBS with 0.1% BSA and 2 mM EDTA at desired densities for Drop-seq.
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bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells (immune cells) and CD31+ (endothelial cells) were then depleted using mouse CD45 and CD31 MicroBeads (Miltenyi Biotec) and the remaining cells were counted and incubated with anti-CD326-APC (eBioscience) ...
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bioRxiv - Genetics 2020Quote: ... NK cell populations using cell-type specific kits (Miltenyi Biotec). For NK cells ...
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bioRxiv - Genetics 2020Quote: ... CD45 positive cells were separated using mouse CD45 microbeads (Miltenyi Biotec). Ter119 positive cells were then isolated from the CD45 negative fraction using mouse Ter119 microbeads (Miltenyi Biotec ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... Enrichment of UCB-derived CD34+ precursors and PB-derived NK cells was accomplished by Magnetic-activated cell sorting using the CD34 MicroBead kit and the NK cell isolation kit (Miltenyi Biotec), respectively.
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Immunology 2021Quote: Spleens of OT-I Hobit reporter × ROSA26-eYFP LT mice were isolated and subsequently CD8+ T cells were isolated by negative selection using MicroBeads (130-104-075, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... To determine proliferation and IFNγ production upon in vitro TCR-mediated stimulation splenocytes were enriched for T cells using CD19 microbeads depletion (Miltenyi Biotec) on LS columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...