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Citations for Miltenyi Biotec :
601 - 650 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Mouse spleen (∼ 0.1g) from 8-12-week-old C57B6 mice was placed in C-Tube (Miltenyi Biotec). After mechanical disruption ...
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bioRxiv - Immunology 2021Quote: ... PBMC were then used to isolate autologous CD3+ T cells using the MACS→ (magnetically activated cell sorting) MicroBead Isolation technique (Miltenyi Biotec, Cologne, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... from control and treated mice were collected and processed for isolation of stromal vascular cells (SVC) using the mouse adipose tissue dissociation kit (Miltenyi Biotec) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... PDOX-derived cells were FACS-sorted (P8, nude control brain)23 or MACS-purified (remaining PDOXs) with a Mouse Cell Depletion kit (Miltenyi Biotec)21 ...
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bioRxiv - Genetics 2023Quote: ... gentle and rapid generation of single-cell suspensions was achieved by using a modified version of the procedure of a mouse Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2023Quote: Primary tumour epithelial cells were obtained using the gentle MACS Dissociator and Mouse Tumor dissociation kit (Miltenyi Biotec, Bisley, Surrey, UK) following the protocol recommended for ‘Dissociation of Tough Tumors’ ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from differently treated mice were isolated using CD4+ microbeads according to manufacturer’s instruction (Miltenyi Biotec). The purity of CD4+ T cells was analyzed by flow cytometry after the staining with anti-CD4 antibody and reported to be > 95% ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from the spleen and peripheral lymph nodes using anti-CD4 microbeads (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Immunology 2022Quote: ... both naïve and memory OT-1 CD8 T cell donors were magnetically sorted via magnetic beads (Miltenyi Biotec) with specific antibodies ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by negative selection from spleens and lymph nodes (Miltenyi Biotec, #130-104-454) according to manufacturer’s instructions (typically >95% purity) ...
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bioRxiv - Cancer Biology 2022Quote: ... fresh splenocytes were isolated as previously described and subjected to Pan-T cell magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Immunology 2021Quote: Naïve CD4 T cells were isolated from mice spleens and lymph nodes by magnetic bead separation (Miltenyi Biotec) following the manufacturers’ protocols ...
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... the enriched CD8+ T cells were stimulated with the CMVpp65 overlapping peptide pool PepTivator® CMVpp65 (Miltenyi Biotec) at a concentration of 1 µg of each peptide/ml for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... Purity of CD4+ T cell population was measured by flow cytometry (MACSquant Analyzer using MACSQuantify software, Miltenyi Biotec), showing a fraction of at least 95% CD4+CD3+ double positive cells and less than 0.01% CD123+CD304+CD303+ cells ...
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bioRxiv - Microbiology 2024Quote: Primary CD4+ T cells were purified from PBMCs of a healthy donor by negative selection (Miltenyi Biotec, USA). Purified CD4+ T cells were stimulated with 1 µg/mL soluble anti-CD3 (clone OKT3 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Immunology 2019Quote: ... CD56+ NK cells (mouse anti-human CD56, Miltenyi Biotec) and CD19+ B cells (mouse anti-human CD19 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transferred in gentleMACS C-tubes containing RPMI/DMEM media and kit enzymes (tumor dissociation kit, Miltenyi Biotec, Gladbach, Germany) and were broken down using the gentleMACS ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell suspensions of CD4+ or CD8+ T-cells were prepared from spleens by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Naive 6-week-old IFN-αβR-/- mice (n = 4-9 ...
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bioRxiv - Genomics 2024Quote: ... tissues from each mouse were transferred to a chilled gentleMACS C Tube (Miltenyi Biotec cat. #130-093-237) with 2 mL Nuclei Extraction Buffer (Miltenyi Biotec cat ...
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bioRxiv - Cancer Biology 2021Quote: ... with the mouse lung dissociation kit (130-095-927, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Lungs were digested using the Mouse Lung Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissociated using the mouse Lung Dissociation Kit (Miltenyi Biotec) and the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Physiology 2023Quote: ... The supernatant was incubated with MicroBeads (Miltenyi Biotec Pan Kit, mouse), then the magnetic separation and elution were performed on the next day.
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bioRxiv - Immunology 2023Quote: ... The "Mouse Tregs detection kit" (Miltenyi Biotec®, Bergisch Gladbach, Germany) was used for regulatory T-cell staining ...
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bioRxiv - Microbiology 2024Quote: ... Neutrophils were isolated using a mouse neutrophil isolation kit (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the mouse Tumor-Associated Fibroblast Isolation Kit was used (Miltenyi Biotec); isolation was performed according to manufacturer instructions applying a two-step strategy ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched from spleen with CD4 microbeads (Clone: L3-T4, Miltenyi Biotec, San Diego, CA, USA). The enriched CD4+ T cells were stained with antibodies to define TFH cells as described in flow methods below ...
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bioRxiv - Systems Biology 2022Quote: ... CD8+ T cells were isolated by negative selection using magnetic beads following the instructions from the manufacturer (Miltenyi Biotec). The purified CD8+ T cells were incubated with APC-conjugated HLA-A2/YFV NS4b (LLWNGPMAV ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive selection was performed to isolate CD8+ T cells from primary tumors and spleens using CD8a microbeads (Miltenyi Biotec), an LS column ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQUANT Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQuant Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: hCD8+ T cells were isolated from commercially available sources (buffy coats from ZenBio Inc. and leukopaks from Miltenyi Biotec) using magnetic separation (positive selection ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...