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Citations for Miltenyi Biotec :
6601 - 6650 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... done using LD columns and a CD25 depletion kit (Miltenyi Biotec), CD4+CD25− cells were activated with plate-bound anti-CD3 (500 ng/24-well culture plate well ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with 10 ng/ml hIL-4 (Miltenyi Biotec, Germany) and treated with anti-hIL4 scFv (Creative Biolabs ...
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bioRxiv - Immunology 2021Quote: ... CD45- cells were enriched with magnetic-bead-conjugated anti-CD45 antibody (Miltenyi Biotec) before multicolor staining for flow cytometric cell sorting.
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bioRxiv - Immunology 2021Quote: ... Lungs underwent an initial mechanical dissociation using the m_lung_01_01 program on the gentleMACS dissociator (Miltenyi Biotec). Lung tissues were then digested with 50 μg/mL Liberase TM (Roche ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2021Quote: ... Loosely adherent BMDCs were collected and further purified with CD11C+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) to obtain a purity of >90% ...
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bioRxiv - Immunology 2021Quote: ... and Ter119 APC- or PE-labeled antibodies followed by magnetic depletion with anti-APC or -PE Micro-Beads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... CD344-PE/Vio770 (Clone CH3A4A7, Miltenyi Biotec). For the LEGENDScreen™ human cell Screening kit ...
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bioRxiv - Immunology 2021Quote: ... FcεRI-FITC (clone CRA1, Miltenyi Biotec, Bergisch Gladbach, Germany), FceRI-PE (clone CRA1 ...
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bioRxiv - Immunology 2021Quote: ... and the gentleMACS Dissociator (Miltenyi Biotec). Cells were then passed through a 70 µm cell strainer and washed ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissociated using the mouse Lung Dissociation Kit (Miltenyi Biotec) and the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... The CD28 MicroBead Kit (130-093-247; Miltenyi Biotec) was used for isolation of CD4+CD28null T-cells from PBMC ...
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bioRxiv - Immunology 2021Quote: ... and CD62L microbeads (Miltenyi Biotec, San Diego, CA) according to the manufacturer’s instructions and confirmed >90% pure by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified using density gradient centrifugation over Ficoll-Paque and monocytes isolated with subsequent CD14 MACS sorting (Miltenyi Biotec, Bergisch Gladsbach, Germany) as described previously [22 ...
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bioRxiv - Immunology 2021Quote: ... CD5 APC (Miltenyi Biotec), CD11c BUV395 and Siglec F AF647 (all Becton Dickinson).
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bioRxiv - Immunology 2021Quote: ... PBMC cell suspensions were pre-treated with anti-CD19-conjugated microbeads (Miltenyi Biotec, CA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Using an NHP specific CD20 positive selection isolation kit (Miltenyi Biotec; Figure S1), we isolated CD20+ cells.
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bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
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bioRxiv - Immunology 2021Quote: ... and underwent negative selection using an autoMACS Pro Separator (Miltenyi Biotec). The purity of the resulting populations confirmed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... The bone marrow monocytes were isolated using the Murine Bone Marrow Monocyte Isolation kit (Miltenyi Biotec) and underwent negative selection using an autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then isolated from the PBMCs by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were maintained in RPMI 1640 medium supplemented with 1 % human serum ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2021Quote: ... and monocytes were separated from lymphocytes by positive selection using magnetic cell sorting technique according to the manufacturer’s instructions (Miltenyi Biotec) and as described (34).
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec) through a magnetic column.
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bioRxiv - Immunology 2021Quote: ... and blocked with FcR Blocking Reagent (Miltenyi Biotec) for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were enriched using mouse CD3ε Microbead kit (Miltenyi Biotec), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Immunology 2021Quote: ... The cytometric bead array was analysed using the MACSQuant® Analyser (Miltenyi Biotec). Analysis was performed using FlowJo (Treestar).
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Primary murine CD4 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Responder CD8 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) and stained with 5μM CellTrace Violet (CTV ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were enriched by incubating cells with CD4 MicroBeads and positively selecting with MACS cell separation MS columns (Miltenyi Biotec). Purified cells were resuspended in RPMI-C with 500 U/mL recombinant IL-2 (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... or for scRNAseq following an additional step of dead cell removal using the MACS Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... Ly6G+ cells were positively selected to isolate G-MDSCs from Ly6G- M-MDSCs and were passed through LS columns (Miltenyi Biotec, cat. 130-042-401) twice to increase purity ...
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bioRxiv - Immunology 2021Quote: ... and then dissociated using a tumor dissociation kit (Miltenyi Biotec, cat. 130-096-730) and the OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and the OctoDissociator (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then centrifuged and resuspended in buffer containing CD45 MicroBeads (Miltenyi Biotec, Auburn, CA). Cells were incubated with microbeads for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... we sorted 30,000 CD45+ cells from tumors by AutoMACS (Miltenyi Biotec, USA). Sorted cells were stained with TotalSeq mouse Hashtag antibodies (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... the samples were gently incubated with a proprietary enzymatic solution in C-tubes (Miltenyi Biotec, Cat. No.: 130-096-334) at 37°C for at least 30 minutes using a rotator ...
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bioRxiv - Immunology 2021Quote: ... the samples were homogenized using a gentleMACS (Miltenyi Biotec) and the program m_intestine_01 ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were enriched using microbeads (Miltenyi Biotec, Cat. #.: 130-117-043). Naïve CD4+ T cells (CD4+CD44lowCD62LhighCD25− ...
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bioRxiv - Immunology 2021Quote: Intestinal lymphocytes were isolated from RAG1-/- recipients using a lamina propria dissociation kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. No.: 130-097-410). Briefly ...
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bioRxiv - Immunology 2021Quote: NK cells were purified by negative selection from mice splenocytes with an NK cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2021Quote: ... 20μg/mL DNase in 1x HBSS with MgCl2 and CaCl2) and run on the default Lung_01 protocol on a gentleMACS Dissociator instrument (Miltenyi Biotec). Samples were then incubated at 37°C for 30 min and further dissociated with default Lung_02 protocol ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells from MLNs of Hpb cured VillinCre-A2BARfl/fl and corresponding controls VillinCre infected mice were magnetically sorted from MLN and spleen single cell suspensions using mouse CD4 MicroBeads (Miltenyi Biotec, Inc.) sorted on positive selection columns ...
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bioRxiv - Microbiology 2021Quote: ... CD14+ monocytes were purified under positive selection using anti-CD14 microbeads (Miltenyi Biotec, San Diego, CA). For RNA-Seq protocols ...