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Citations for Miltenyi Biotec :
6851 - 6900 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the SVF resuspended in 10 ml room temperature plain DMEM followed by additional filtering through a 30 μm mesh filter (Miltenyi Biotec # 130-041-407) and subsequent centrifugation at 700 g for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Stained cells were also quantified by Flow-cytometry in a MACSQuant-VYB (Miltenyi Biotec, Surrey, UK). Data analysis was carried out by using the FlowJo software ...
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bioRxiv - Cell Biology 2020Quote: ... WBM was depleted of lineage+ cells using a Lineage Cell Depletion Kit (Miltenyi Biotec), surface-stained using antibodies raised against cKIT (2B8 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
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bioRxiv - Cell Biology 2020Quote: ... WBM was depleted of terminally differentiated hematopoietic cells using a murine Lineage Cell Depletion Kit (Miltenyi Biotec 130-090-858) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... CD146+ pericytes were isolated from digested cell suspensions of Scx+ or ScxGFP mice using anti-CD146 magnetic microbeads (130-092-007, Miltenyi Biotec, San Diego, CA, USA), and placed into 24-well tissue culture dishes (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was incubated for 1 h on ice with anti-GFP magnetic beads (Miltenyi Biotec), and beads were washed 4 times with extraction buffer on the magnetic field ...
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bioRxiv - Cell Biology 2020Quote: ... using a magnetic cell separation device (QuadroMACS Separator;130-090-976, Miltenyi Biotec). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Lineage negative and CD31 positive BMCs were isolated using first immunomagnetic Lineage Cell Depletion Kit (130-092-211, Miltenyi Biotec) followed by positive selection with CD31 MicroBead Kit (130-091-935 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by positive selection with CD31 MicroBead Kit (130-091-935, Miltenyi Biotec) using a magnetic cell separation device (QuadroMACS Separator;130-090-976 ...
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bioRxiv - Cell Biology 2020Quote: ... APC- conjugated AC133 (130-090-826, Miltenyi Biotec), PE-Cy7-conjugated CD34 (343516 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were separated on LS columns (130-042- 401, Miltenyi Biotec) for positive and negative selection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were separated on LS columns (130-042-401, Miltenyi Biotec) for positive and negative selection ...
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bioRxiv - Cell Biology 2020Quote: The isolation of Thy1 positive EVs is performed by positive selection using anti-CD90 MicroBeads (Miltenyi Biotec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
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bioRxiv - Cell Biology 2020Quote: ... A microcolumn (LS separation columns, MACS, Miltenyi Biotec) was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution ...
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bioRxiv - Cell Biology 2020Quote: ... and labelled with CD11b-FITC conjugate (Miltenyi Biotec, Bergisch Gladbach, Germany) for microglial isolation (FACSAria III ...
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bioRxiv - Cell Biology 2020Quote: ... Lungs were homogenized RIPA buffer with protease inhibitor using a tissue dissociator (GentleMacs Dissociator Miltenyi Biotec). Homogenates were then centrifuged at 10,000 rpm and 4°C and the supernatants were collected and prepared for either Lc3 or Atg16L1 detection as described above.
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bioRxiv - Cell Biology 2020Quote: ... using a gentleMACS dissociator (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Subsequently the lysates were centrifuged for 30 min at 16,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA in PBS) and endothelial cells (CD31+) and leukocytes (CD45+) were sequentially positively selected using CD31 and CD45 MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Total proximal or distal dissociated cells were passed through a series of cell strainers of decreasing pore sizes from 500μm to 40μm under vacuum pressure and depleted of immune and endothelial cells by magnetic associated cell sorting (MACS) in accordance to the manufacturing protocol (Miltenyi Biotec). Viable epithelial cells were further enriched by fluorescence associated cell sorting (FACS ...
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bioRxiv - Cell Biology 2020Quote: ... MACS positive and negative cells were collected by magnetic-activated cell sorting (MACS) according to manufacturer’s instructions (Miltenyi Biotec). Cells were plated at 50,000 cells per well in a 12 well plate for MACS positive and negative cells and 5,000 cells per well for unsorted cells for CFU-F assessment.
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bioRxiv - Cell Biology 2020Quote: ... were isolated using LS columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Stro-1 positive cells were collected by magnetic-activated cell sorting (MACS) according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: ... CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using LS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with magnetic bead-conjugated secondary antibody (Miltenyi Biotec). After further washes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with magnetic bead-conjugated secondary antibody (Miltenyi Biotec) for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... All PSCs were cultivated on Matrigel (BD Bioscience)-coated plates using: StemMACS iPS-Brew XF medium (Miltenyi Biotec GmbH, #130-104-368), supplemented with 0.1 mg/ml Pen/Strep (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ng/mL EGF (Miltenyi Biotec). Half of the medium was changed every day until the first colonies appeared ...
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bioRxiv - Cell Biology 2020Quote: ... and 25 ng/ml M-CSF (Miltenyi Biotec). Differentiation was obtained after 6 days of culture ...
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bioRxiv - Cell Biology 2020Quote: ... live cells were enriched via MACS dead cell removal kit (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Data were acquired on a MACSQuant VYB (Miltenyi Biotec) and cell populations were analyzed with FlowJo 10.
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bioRxiv - Cell Biology 2020Quote: ... in PBS using the gentleMACS™ C Tubes (Miltenyi Biotec, 130-096-334) in combination with the gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Neutrophils were purified using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec 130-104-434) followed by erythrocyte depletion (Miltenyi Biotec 130-098-196 ...
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bioRxiv - Biochemistry 2020Quote: ... followed by erythrocyte depletion (Miltenyi Biotec 130-098-196) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec). T cells were first cultured for three days using stimulating conditions (RPMI supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted cells were treated with 50 ng/mL recombinant stem cell factor (SCF; Miltenyi Biotec), 5 μM imatinib mesylate (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Plant Biology 2020Quote: ... amino acids 98-106 (Miltenyi Biotec, 130-091-972; 1:10.000) or anti-GFP (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-GFP (Miltenyi Biotec, 130-091-833; 1:10.000), and detected using Signal West Pico Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Bone marrow cells were cultured (0.5×106 cells per mL) in the presence of 20 ng/mL GM-CSF (Miltenyi Biotec) in complete RPMI medium ...
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bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...