Labshake search
Citations for Miltenyi Biotec :
601 - 650 of 656 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Surface staining was performed for 15 min in the presence of 1 mg/ml of Beriglobin (CSL Behring) with the following fluorochrome-conjugated antibodies titrated to their optimal concentrations: anti-CD3-FITC (Miltenyi Biotec), anti-CD4-VioGreen (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2024Quote: ... day 27 cultures were first enriched for motor neurons by using antibodies against PSA-NCAM and L1CAM (both from Miltenyi Biotec) [39] ...
-
bioRxiv - Cell Biology 2024Quote: ... proteins from the native gel were transferred to the PVDF membrane and probed with anti-CD95 antibody (Miltenyi Biotec, clone DX2).
-
bioRxiv - Cell Biology 2024Quote: ... The bead-EV complexes were resuspended in 100 µL/sample of filtered PBS and incubated with the following conjugated monoclonal antibodies from Miltenyi Biotec: mouse anti-CD9-APC antibody (Clone REA1071) ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
-
bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
-
bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
-
bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
-
bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
-
bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
-
bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
-
bioRxiv - Molecular Biology 2024Quote: ... CD45+ dynabeads were then removed by placing the sample on a magnetic stand and the remaining cells (CD45-) were incubated in CD140b-APC antibody (Miltenyi Biotec, Germany) for 30 minutes at 4°C on a shaker ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells from cord blood or adult donors were sorted using microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (Miltenyi Biotec MACS) through positive magnetic bead selection on LS columns.
-
bioRxiv - Neuroscience 2024Quote: ... In most cases microglial cells were removed using a mini MACS separator based on an anti-rat CD11b/c antibody conjugated to magnetic microbeads according to manufacturer instructions (Miltenyi Biotec, CA) (Marek et al. ...
-
bioRxiv - Developmental Biology 2024Quote: ... the homogenate was incubated with microbeads conjugated to a cocktail of monoclonal antibodies against no-target cells (Miltenyi Biotec, Bergisch Gladbach, Germany) at 2-8 °C for 20 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
-
bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... Antibody staining for FACS was performed in Flow Cytometry Staining Buffer (ThermoFischer Scientific) and following antibodies were used: PE-GLAST (ACSA-1, Miltenyi Biotec, 1/40), APC-CD133 (13A4 ...
-
bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
-
bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
-
bioRxiv - Immunology 2020Quote: Monocytes were purified from freshly thawed PBMC using CD14 antibodies conjugated to magnetic microbeads per the manufacturer’s recommendations (Miltenyi Biotec, San Diego CA). Magnetically bound monocytes were washed and eluted for collection ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and monocytes were magnetically sorted with magnetic microbeads coupled with antibodies directed against CD14 (Miltenyi Biotec #130-050-201). Monocytes were then seeded on glass coverslips at 1.5×106 cells/well in six-well plates in RPMI 1640 (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed and stained for 10 minutes at room temperature with an intracellular stain cocktail containing the following antibodies: IFN-γ APC and TNF-α PE (all from Miltenyi Biotec). Cells were analyzed on a MACSQuant10 flow cytometer (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
-
bioRxiv - Physiology 2023Quote: ... Lung ECs were then isolated using two step magnetic bead separation (CD45 and CD31 antibody- conjugated microbeads; Miltenyi Biotec Inc., Auburn, CA, USA). For primary SMCs isolation ...
-
bioRxiv - Cancer Biology 2021Quote: ... also known as LPEC-1 and -6 in previous studies) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) 10-13 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
-
bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
-
bioRxiv - Immunology 2021Quote: B cells were purified from splenocytes by positive selection using magnetic beads-conjugated monoclonal anti-mouse B220 antibody (clone: RA3-6B2, Miltenyi Biotec, Bergisch Gladbach, Germany). Isolated B cells (5 × 105/mL ...
-
bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
-
bioRxiv - Pathology 2023Quote: ... CD14 + monocytes were then isolated from the PBMCs by positive selection using magnetic beads conjugated to anti-CD14 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany).
-
bioRxiv - Cancer Biology 2023Quote: CD40 stimulation medium were prepared by incubation of 40 uL of CD40 ligand and 40 uL of cross-linking antibody (Miltenyi Biotec 130-106-196) mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196) ...
-
bioRxiv - Plant Biology 2022Quote: ... isolated from transfected protoplasts were separated by SDS-PAGE and subjected to immunoblotting using an anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000).
-
bioRxiv - Physiology 2023Quote: ... the cells were mixed with CD45 antibody-coated magnetic beads (CD45 MicroBeads, 130-052-301) and then passed through a MACS LD column (Miltenyi Biotec, 130-042-901) on a MidiMACSTM separator (Miltenyi Biotec ...
-
bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
-
bioRxiv - Neuroscience 2024Quote: ... The monocyte population was enriched by positive selection of CD14-labeled target cells using a human magnetic antibody cell sorting (MACS) system (Miltenyi Biotec, Bergisch-Gladbach, Germany), according to the manufactureŕs procedure ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The HA-Bcl-2 -scFvPZ1 domain for the dual-DROP-CAR was labeled with an anti-HA antibody (1:100, Miltenyi Biotec, 130-120-791) and an anti-mouse secondary antibody (1:100 ...
-
bioRxiv - Cell Biology 2021Quote: ... The hiPSC-CMs were incubated with the conjugated antibodies cTnT-Vioblue or cTnT-FITC (1:50, Miltenyi Biotec, #130-120-402 or #130-119-575). All antibodies were diluted in permeabilization medium (medium B ...
-
bioRxiv - Immunology 2020Quote: Peritoneal B1a cells were obtained through negative magnetic-activated cell separation with a cocktail of antibodies depleting other than B1a cells achieving more than 90% purity in isolation (Miltenyi Biotec B.V., Utrecht, The Netherlands). B1a cells were subsequently counted and plated in IMDM medium (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
-
bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were positively selected using anti-CD4 and CD8 antibodies through the Prodigy TCT program on a CliniMACS Prodigy (Miltenyi Biotec, Cat# 200-075-301). After selection ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were then magnetically sorted to enrich CD14+ cells using microbeads conjugated to a monoclonal human CD14 antibody (Miltenyi Biotec; Cat No. 130-050-201). Cryopreserved Beagle canine CD14+ ...