Labshake search
Citations for Miltenyi Biotec :
551 - 600 of 656 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
-
bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770 ...
-
bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation was carried out using micromagnetic bead-coupled GFP antibodies from µMACS GFP Isolation Kit (Miltenyi Biotec 130-091-125) following the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2024Quote: ... The cells were then stained with fluorochrome-conjugated antibodies (anti-mouse-BV421-CD45) and anti-mouse-PE-O4 (Miltenyi Biotec)) for 40 min at 4°C to characterize oligodendrocytes (CD45-O4+) ...
-
bioRxiv - Physiology 2024Quote: ... Cells were filtered through 35 μm filters and incubated with CD31 magnetic conjugated bead antibodies (#130-097-418, Miltenyi Biotec). Positive selection for CD31+ cells was performed using magnetic separator columns (#130-042-201 ...
-
bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
-
bioRxiv - Cancer Biology 2021Quote: Murine liver primary ECs were isolated from athymic nude mice also using MACS microbead-conjugated antibodies for murine CD31 (Miltenyi Biotec) and cultured in MV2 EC culture medium as described above ...
-
bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and incubated in 2% FBS in PBS with anti-ROR1-APC antibody (1:25, #130-119-860, Miltenyi Biotec) on ice for 30 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
-
bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...
-
bioRxiv - Neuroscience 2021Quote: ... and stained with fluorophore conjugated antibodies (CD11b-AF700, CD45-FITC, BD Pharmingen 557690 and 553080, C5aR-PE, CD68-PE and CD107a-PE, Miltenyi Biotec 130-106-174 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... slides were incubated for 1 h at room temperature in the dark with a cardiac troponin-T antibody conjugated to APC (130-120-543; Miltenyi Biotec) prepared at a 1:100 dilution in PBS ...
-
bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were enriched from the filtrates by Ficoll density gradient centrifugation and magnetic-activated cell sorting using CD34 antibody-conjugated microbeads (Miltenyi Biotec). CD34+ cells were grown in serum-free StemSpan SFEMII (Stemcell Technologies ...
-
bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
-
bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...
-
bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
-
bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs) for either the 8-color MACSQuant Analyzer (Miltenyi Biotec), or the 14-color LSRFortessa™ X-20 flow-cytometers (BD) ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human B cells from PBMC were labelled with B Cell lineage-specific CD19 antibodies and removed by magnetic-activated cell sorting by using the Lineage Cell Depletion Kit for human cells from Miltenyi Biotec Inc (Auburn ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
-
bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
bioRxiv - Immunology 2021Quote: ... Cells were recovered via centrifugation and stained as described above using a biotinylated monoclonal antibody against TIM4 (Miltenyi Biotec, clone: REA999), the isolated with anti-biotin Microbeads (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures with the surface antibody CD34-PE (clone AC136, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1 hour on ice with magnetic beads coated with antibodies against Pdgfra (Miltenyi Biotec #130-101-547). Separation of Pdgfra-positive and negative populations was performed with LS column (Miltenyi Biotec #130-042-401 ...
-
bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
-
bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
-
bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
-
bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
-
bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
-
bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
-
bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
-
bioRxiv - Cancer Biology 2023Quote: ... human T-ALL cells were purified after labeling with anti-huCD45 PE conjugated antibody followed by immuno magnetic selection with anti-Phycoerythrin (PE) microbeads (130-048-801, Miltenyi Biotec).
-
bioRxiv - Cell Biology 2024Quote: ... 100 μL of each sample was removed and set aside as representative “crude mitochondrial fraction.” The remaining 300 μL was diluted to 2 mL total with ICB and combined with 60 μL of superparamagnetic microbeads coated with mouse IgG1 antibodies against GFP (#130-091-125; Miltenyi Biotec). These samples were then placed on a rotating wheel at +4°C for 1 hr ...
-
bioRxiv - Plant Biology 2024Quote: ... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
-
bioRxiv - Immunology 2024Quote: ... Surface staining was performed for 15 min in the presence of 1 mg/ml of Beriglobin (CSL Behring) with the following fluorochrome-conjugated antibodies titrated to their optimal concentrations: anti-CD3-FITC (Miltenyi Biotec), anti-CD4-VioGreen (Miltenyi Biotec) ...