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Citations for Miltenyi Biotec :
501 - 550 of 687 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in MACS staining buffer and passed through LS columns with a dead cell removal magnetic bead kit (Miltenyi Biotec). CD11b+-positive selection magnetic beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: SB CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Immunology 2023Quote: LV CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec)53 ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
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bioRxiv - Immunology 2022Quote: ... DCs were isolated from murine lymph nodes using magnetic beads according to the manufacturer's instructions (Miltenyi Biotec, Cat.No. 130-100-875). OT-1 T cells were isolated from peripheral blood of OT-1 mice and labeled with 0.5 μM CFSE (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1 hour on ice with magnetic beads coated with antibodies against Pdgfra (Miltenyi Biotec #130-101-547). Separation of Pdgfra-positive and negative populations was performed with LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Cell Biology 2022Quote: CD34+ cells were isolated from umbilical cord blood or peripheral blood by positive selection using immunomagnetic bead cell-sorting system (AutoMacs; Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... PBMCs were depleted of CD4 and CD8 using standard MACs magnetic beads (CD4: 130-045-101, CD8: 130-045-201, Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the mixture of unlabeled RNA and biotinylated RNA was incubated with streptavidin beads from uMACS Streptavidin kits (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution; Miltenyi Biotec), TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatants passed through a 40-μm cell filter and subsequently incubated with 100 µl anti-GFP magnetic beads (Miltenyi Biotec) at 4°C for one h ...
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bioRxiv - Physiology 2024Quote: Primary human monocytes were isolated from healthy human blood samples using CD14-magnetic beads (cat. no.: 130-050-201, Miltenyi Biotec). In brief ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets of Treg and non-Treg cells were collected and stored at -80°C directly after isolation or after 72h stimulation using human Treg Suppression Inspector beads (130-092-909, Miltenyi Biotec) in X-VIVO 15 (02-060F ...
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bioRxiv - Immunology 2024Quote: ... T cells from Buffy coats of healthy donors were isolated by Ficoll separation followed by CD8- and CD4-magnetic bead isolation according to protocol (Miltenyi Biotec). After activation with plate-bound OKT-3 (30ng/μl ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured at 37°C ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... CD4+ T cells were selected from resultant suspensions by labeling with bead conjugated anti-CD4 for enrichment by autoMACs (Miltenyi Biotec). Studies were approved by the Institutional Review Board at Baylor College of Medicine and informed consent was obtained from all patients.
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bioRxiv - Bioengineering 2024Quote: Different sample types were subjected to bead-based multiplex EV analysis by flow cytometry (MACSPlex EV IO Kit, human, Miltenyi Biotec). Unless indicated otherwise ...
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bioRxiv - Immunology 2024Quote: ... 4 days later peritoneal washes were collected and F4/80+ macrophages were sorted with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) with the typical purity yield >95%.
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bioRxiv - Microbiology 2024Quote: ... Stained and MACS bead-bound bacteria unbound to the magnet were collected (20∼40 μl) and passed through MACS molecular columns (Miltenyi Biotec) (one sample/column) ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and pDCs were positively selected from PBMCs using BDCA-4-magnetic beads (MACS Miltenyi Biotec), as previously described (Decembre et al. ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were enriched by depletion of CD43+ cells with anti-CD43-conjugated magnetic beads (MACS anti-mouse CD43; Miltenyi Biotec). Resultant populations were >97% B cells based on B220 staining and FACS analysis ...
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bioRxiv - Cell Biology 2024Quote: Surface protein profiling of primary and TERT EVs was performed via Multiplex bead-based EV analysis followed by a flow cytometric analysis (MACSPlex EV IO Kit, human, 130-108-813, Miltenyi Biotec). EV-containing samples were processed as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Biotin-labeled lineage negative cells (CD45+, CD11b+, CD31+, Sca1+ cells) were either depleted using streptavidin beads (catalog number: 130-048-101, Miltenyi Biotec) through magnetic field or excluded during sorting by using streptavidin-APC-Cy7 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were passaged every 5-7 days using Stem-MACS Passaging Solution (Miltenyi Biotec). Differentiation of SV20 into cardiomyocytes (iCMs ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5 mL of digesting enzyme included in a Tumor Dissociation Kit (Miltenyi Biotec). The tissues were then converted into single-cell suspensions using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Immunology 2021Quote: ... Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec, USA). To obtain high purity Treg ...
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bioRxiv - Immunology 2020Quote: Human monocytes were isolated from peripheral blood mononuclear cells from healthy donors (convention n°7828, Etablissement Français du Sang, Marseille, France) following CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch, Germany) as previously described (19) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Physiology 2023Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Genomics 2024Quote: ... Myelin were removed from the single-cell suspensions using negative selection with Myelin Removal Beads II (Miltenyi Biotec cat. #130-096-733) and LS columns (Miltenyi Biotec cat ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...