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Citations for Miltenyi Biotec :
451 - 500 of 687 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells labeled with MACS beads are captured by the magnetic field of the separator (130-090-312, Miltenyi Biotec Inc.), whereas unlabeled cells pass the magnetic field and end up in the flow through fraction ...
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bioRxiv - Immunology 2024Quote: ... cells labeled with MACS beads are captured by the magnetic field of the separator (130-090-312, Miltenyi Biotec Inc), whereas unlabeled cells pass the magnetic field and end up in the flow through fraction ...
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bioRxiv - Physiology 2024Quote: ... Cells were filtered through 35 μm filters and incubated with CD31 magnetic conjugated bead antibodies (#130-097-418, Miltenyi Biotec). Positive selection for CD31+ cells was performed using magnetic separator columns (#130-042-201 ...
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bioRxiv - Immunology 2024Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... M-MDSCs expanded from bone marrow were collected (see “Bone Marrow Culture”) and sorted via magnetic bead isolation (Miltenyi Biotec) based on manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... and the CD19+ B cells and CD11b+ myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat #: 130-050-301) and CD11b beads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... where NCSCs were positively isolated from the mixed culture using p75-conjugated magnetic beads (catalogue#: 130-097-127, Miltenyi Biotec). Day 15 cells were collected with Accutase ...
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bioRxiv - Neuroscience 2020Quote: ... we added an ice-cold buffer of 5% autoMACS Rinsing Solution (Miltenyi Biotec) in 1X MACS BSA solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 5 ng/mL of IL-15 (130-093-955; Miltenyi Biotec), 1X Glutamax and 100 U/mL penicillin-streptomycin.
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bioRxiv - Cell Biology 2024Quote: ... and SSEA-5 (+) cells were collected using an autoMACS Pro Separator (Miltenyi biotec). Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara ...
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bioRxiv - Genomics 2023Quote: ... Up to 5×109 PBMC were incubated with anti-CD14 microbeads (Miltenyi Biotec) for 30 minutes at room temperature and magnetically enriched using the CliniMACS system (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 min and Anti-Biotin MicroBeads (Miltenyi Biotec, Cat # 130-090-485) for 10 min at 2-8°C ...
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bioRxiv - Immunology 2020Quote: CD4+ cells from the spleen of mice were purified using LS positive selection magnetic-activated columns (MACs) and anti-CD4 (L3T4) beads (Miltenyi Biotec). CD4+ MACs sorted cells were further purified by fluorescence activated cell sorting using a FACS Aria (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... treated with erythrocyte lysis buffer and CD45+ leukocytes were isolated using LS columns and CD45 MicroBeads (35 μL beads per sample, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Genetics 2020Quote: ... Mobilized peripheral blood CD34+ cells were obtained from three de-identified healthy donors (Key Biologics, Lifeblood) and enriched by immunomagnetic bead selection using an AutoMACS instrument (Miltenyi Biotec). Cryopreserved CD34+ cells were thawed and pre-stimulated for 48 h in StemSpan SFEM (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Stained and MACS bead-bound bacteria unbound to magnet plate were collected (20∼40 μl) and passed through MACS molecular columns (Miltenyi Biotec) (one sample/column ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Cell Biology 2022Quote: ... The CD8+ T cells were purified from PBMCs using negative selection bead binding (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec). The following compounds were used at the indicated concentrations ...
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were isolated from PBMC from healthy subjects by positive selection withanti-CD14 magnetic labeling beads and an isolation column (Miltenyi Biotec). Monocytes were resuspended in RPMI 1640 medium (Gibco ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... Supernatant was aspirated and the pellet was resuspended in 10μL Miltenyi Myelin Removal Beads II (Miltenyi Biotec Cat #: 130-096-733) and 90 μL MACS Buffer (0.5% BSA in HBSS w/o Ca2+/Mg2+ ...
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bioRxiv - Neuroscience 2022Quote: ... with HBSS buffer with calcium and magnesium and prepared for myelin removal process (Myelin Removal Beads II, #130-096-733; Miltenyi Biotec). Then ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were passed through a 45 μm Nitex mesh to remove undissociated cell clumps and after addition of myelin removal beads (Miltenyi Biotec), passed through a MACS column (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec) 85 ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence spillover compensation for cell sorting was performed using lymphocytes stained with CD4 (OKT4) in the respective colors and with compensation beads (Miltenyi Biotec) for flow cytometry ...
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bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMCs by a magnetic bead “negative” isolation procedure using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD25+CD127low cells were isolated from a healthy donor and expanded for 21 days using anti-CD3 and anti-CD28-coated beads (Miltenyi Biotec) and 300 units/mL rhIL-2 (Chiron) ...
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bioRxiv - Immunology 2021Quote: ... WKy CD4+CD25high cells were isolated by magnetic and fluorescence-activated cell sorting and expanded with anti-CD3 and anti-CD28 coated beads (Miltenyi Biotec) and 1000 units/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
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bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Four days later fresh DC culture medium was added and on day 8-9 of differentiation suspension and loosely adherent cells were harvested with gentle pipetting and HLA-DR+ cells were isolated by immunomagnetic bead selection (MACS) with HLA-DR microbeads (Miltenyi Biotec) following the manufacture’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Three mice of 3-6 months were euthanized and subjected to a peritoneal lavage with PBS to extract macrophages subsequently purified using CD11b+ magnetic beads (Cat. 130-049-601, Miltenyi Biotec), following the operative manual ...
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bioRxiv - Cell Biology 2021Quote: ... prior to magnetic bead separation in order to obtain a minimum of 90% CD34+-enriched cell population (human Diamond CD34 isolation kit, Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... naïve CD8+ T cells from 1G4 mice spleen were enriched using MACS beads (Pan T cell isolation kit II and CD8a isolation kit, Miltenyi Biotec) and labeled with 5μM CellTraceTM Violet (CTV ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then negatively selected for CD45 and positively selected for CD117 (c-kit) using magnetic bead separation (Miltenyi Biotec). After selection ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16h ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of CD81- (for all samples) or CD63- (for MS and HC samples) isolation beads (Exosome Isolation Kit, human Miltenyi Biotec) were added to 2 ml of diluted sample ...