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Citations for Viagen Biotech :
51 - 86 of 86 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were generated using cell lysate for each cell line as template (DirectPCR lysis reagent, Viagen), and then purified by using QIAquick 96 PCR Purification Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: Tail tips of mice were lysed in 200 μl of DirectPCR Lysis Reagent (Viagen Biotech, Cat# 102-T) with proteinase K (0.2 mg/ml ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was released from mouse tail biopsies by incubation in DirectPCR Lysis Reagent (Tail) (Viagen Biotech, Inc.) at 85°C ...
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bioRxiv - Neuroscience 2024Quote: ... Tail snips (∼0.5 cm) were digested in 200 μl of DirectPCR Lysis Reagent (Viagen Biotech, Los Angeles, CA) and 2μl of Proteinase K (Zymo Research ...
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bioRxiv - Cancer Biology 2024Quote: Tail snips collected from 2.5-week-old mice were lysed in DirectPCR Lysis Reagent (Viagen Biotech, 102-T) and Proteinase K (Millipore Sigma ...
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bioRxiv - Genetics 2021Quote: The toe clips or ear notches from the offspring were lysed in 30 μl of DirectPCR Lysis Reagent (Viagen) with 1 μl of proteinase-K (20 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... Mice were routinely genotyped using genomic DNA extracted from tail biopsies using the Direct PCR Lysis tail reagent (Viagen) supplemented with 5 mg/ml proteinase K (Worthington) ...
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bioRxiv - Cell Biology 2022Quote: ... Mice were routinely genotyped using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5□mg/mL proteinase K (Worthington) ...
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bioRxiv - Neuroscience 2023Quote: ... All genotyping was performed in house using ear punch tissue lysed overnight in DirectPCR Lysis Reagent (Viagen, 102-T) and Proteinase K (Sigma ...
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bioRxiv - Genetics 2021Quote: ... cells were washed with 1x Ca2+/Mg2+-free PBS and resuspended in 50 μl of DirectPCR Lysis Reagent (Viagen Biotech) containing 0.4 mg/ml Proteinase K (Sigma ...
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bioRxiv - Genetics 2021Quote: Crude lysates for conventional PCR genotyping were prepared from ear biopsies or yolk sacs using DirectPCR Lysis Reagent (Viagen Biotech). Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was removed prior to the addition of 20 μL DirectPCR Lysis Reagent Cell (Viagen, cat. no. 301-C) supplied with 1:100 10 mg/mL Proteinase K (Bioline ...
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bioRxiv - Cell Biology 2023Quote: ... by their overnight incubation at 56°C followed by 1 hour at 85°C in DirectPCR (Tail) Lysis Reagent (Viagen) supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2023Quote: Mice were tail clipped upon weaning and the tail samples were dissolved in DirectPCR Lysis Reagent (Viagen Biotech; #102-T), as outlined in the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Mice were genotyped by NGS using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5 mg/mL proteinase K (Worthington) ...
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bioRxiv - Genomics 2023Quote: ... Individual nematode was isolated and washed with 10% bleach for 10 seconds and transferred into with 200µl PCR tube containing lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Cancer Biology 2023Quote: In-house genotyping was done using DNA extracted from tail or ear tissue using DirectPCR Lysis Reagent (Viagen Biotech, Inc). Thermal cycling was performed using the Power SYBR Green 2X Master Mix (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... Established colonies were manually picked and expanded for screening indel mutations using PCR amplification of a region spanning the targeted gRNA region (genomic DNA isolated using DirectPCR Lysis Reagent (Tail) (VIAGEN), primers ...
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bioRxiv - Cell Biology 2020Quote: ... Genotyping of LRP5 KO and AXIN2 KO mutants was done following genomic DNA isolation (DirectPCR Lysis Reagent; 301-C, Viagen Biotech) by PCR using DreamTaq DNA Polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Genomic DNA was extracted from virally transduced HUVEC cells by: collecting approximately 10,000 cells, washing in PBS (sigma-Aldrich, US) and then lysing with DirectPCR Lysis Reagent (Viagen Biotech, US) containing Proteinase K (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated by enzymatic digestion of tail clips using Direct-PCR Lysis Reagent (Viagen Biotech, Los Angeles, CA, USA). Genotypes were determined by performing PCR amplification of genomic DNA using allele-specific primers ...
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bioRxiv - Cell Biology 2020Quote: ... Genotyping was carried out on genomic DNA derived from adult and embryonic tails using the DirectPCR Lysis Reagent (102-T, Viagen Biotech) and EconoTaq Plus Green 2X Master Mix (Lucigen) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with 500 μL cold PBS and then lysed with 100 μL DirectPCR Lysis Reagent (Viagen Biotech, 102-T) with 1% Proteinase K (Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Genomic DNA (obtained from a 2mm ear punch) was extracted using DirectPCR Lysis Reagent (Mouse tail) (Viagen Biotech, Los Angeles, CA) and PCR-grade proteinase K (Roche #RPROTK-RO ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic tail snips or lung tissue was incubated in 0.2mg/mL Proteinase K in DirectPCR Lysis Reagent (Viagen Biotech 101-T) at 55°C for 4 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Crude extracts were prepared for each clone: cells from a full 96-plate well were lysed in 25 µL DirecPCR Lysis Reagent (302-C, Viagen Biotec) supplemented with 100 µg/mL proteinase K and incubated at 55°C ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Microbiology 2022Quote: Crude lysates of single worms were produced as follows: 10 μl lysis buffer (100X stock solution: 1000 μl Direct PCR Lysis Reagent (Cell) (Viagen, 301-C), 50 μl 1 M DTT (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping of Fgd4fl/+ mice was performed by PCR on DNA isolated from tail biopsies using Direct PCR lysis reagent (Viagen Biotech Inc., USA), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The mice genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were transferred to 96-well plates to be expanded and genotyped by PCR after crude DNA extraction with the DirectPCR Lysis Reagent (Viagen Biotech #302-C). Clones with a correct insert were further verified by Sanger sequencing and Western blot.
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bioRxiv - Molecular Biology 2022Quote: ... Knock-out efficiency at the polyclonal level and frameshift confirmation of the individual clones was performed by isolating the cell genomic DNA using the DirectPCR Lysis Reagent (Viagen Biotech, Los Angeles, USA) and treatment with 0.36 mg/mL of proteinase K ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA was isolated from toe clips by incubating in DirectPCR (Tail) Lysis Reagent for Genotyping Crude Lysates (cat# 102-T; Viagen Biotech, Los Angeles, CA, USA) with proteinase K (cat# DSP50220-0.1 ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was isolated from a 5 mm tail biopsy using a rapid digestion method where 95 μl of lysis buffer reagent (Viagen Biotech, Los Angeles, CA; Cat: 102-T) and 5 μl of Proteinase K (20 mg/ml ...