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Citations for Viagen Biotech :
1 - 50 of 86 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... cells were lysed in DirectPCR lysis reagent (Viagen). Lyastes were subsequently assessed by running qPCR with primers annealing to the transposon sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in DirectPCR Lysis Reagent (Viagen Biotech) and Proteinase K in 4:1 ratio ...
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bioRxiv - Immunology 2019Quote: Genomic DNA was extracted using DirectPCR lysis reagent (Viagen) with proteinase K (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed using DirectPCR lysis reagent (Viagen Biotech) according to the manufacturer protocol and lysates were used in a screening PCR to identify edited cells ...
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bioRxiv - Bioengineering 2023Quote: ... was added to DirectPCR Lysis Reagent (Viagen Biotech Inc.) to a final concentration of 50 μg/ml ...
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A pancreas specific Ptf1a-driven Cre mouse line causes paternally transmitted germline recombinationbioRxiv - Genetics 2020Quote: Collected samples are lysed in 200ul DirectPCR Lysis Reagent (Viagen) supplemented with 10ul PCR grade Proteinase K (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in direct PCR reagent (Viagen 301-C) supplemented with 1 μL proteinase-K (Viagen 501-PK) ...
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bioRxiv - Molecular Biology 2019Quote: gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA for PCR was extracted using DirectPCR Lysis Reagent (Viagen Biotech) plus 10 µg/ml proteinase K (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech) with Proteinase K (O/N 55 °C) ...
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bioRxiv - Developmental Biology 2023Quote: Tissue samples were lysed in DirectPCR lysis reagent (cell) (Viagen Biotech), and the LoxCode sequence amplified using a 3-round PCR ...
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bioRxiv - Systems Biology 2024Quote: ... cells were lysed with DirectPCR Lysis Reagent (Viagen Biotech 301-C). Genomic regions were PCR-amplified (Platinum SuperFi II PCR master mix ...
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bioRxiv - Developmental Biology 2019Quote: ... was extracted using DirectPCR Lysis Reagent (Tail) (Viagen Biotech, Los Angeles, CA) and PCR Grade Proteinase K (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... Genomics DNA were prepared using the DirectPCR Lysis Reagent (Viagen Biotech Inc.). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA isolation was performed using DirectPCR Lysis Reagent (Cell) (Viagen Biotech), Proteinase K (EO0491 ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) supplemented with 1:100 10 mg/mL Proteinase K (Bioline) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) and sgRNA target sites were amplified for TIDE analysis as described above (using primers SM8+9 (LAD2) ...
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bioRxiv - Microbiology 2023Quote: ... or DirectPCR™ Lysis Reagent (Direct buffer) (Viagen Biotech Inc., CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted either using DirectPCR Lysis Reagent (Viagen Biotech, 301-C) or Puregene Core Kit B (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... the test plate was washed twice with PBS and DirectPCR lysis reagent (Viagen) mixed 1:1 (vol ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C using DirectPCR Lysis Reagent (mouse tail) (VIG102-T, Viagen Biotech). PCR for Pax7-nGFP was performed using primer Pax7nGFP.F/ Pax7nGFP.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Biophysics 2021Quote: ... U2OS cDNA from selected clones was isolated with DirectPCR Lysis Reagent (Viagen 101-T), treated with 0.5 mg/ml proteinase K for 15 min ...
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bioRxiv - Genetics 2022Quote: ... A fraction of the expanded population was lysed in Direct PCR Lysis reagent (Viagen) with proteinase K (Roche) ...
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bioRxiv - Immunology 2022Quote: Lysis: Sorted cells were lysed in 40 µl DirectPCR Lysis Reagent (Cell) from Viagen Biotech with 0.4 mg Prot K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Cell Biology 2020Quote: ... ear or yolk sac by overnight incubation at 55°C with DirectPCR Lysis Reagent (Viagen) and Proteinase K (New England Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... a snap frozen tumoroid cell pellet was lysed using DirectPCR Lysis Reagent for Cells (Viagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... Lysis buffer was prepared by diluting one part direct-lysis reagent (301-C, Viagen Biotech) in two parts destilled water (e.g ...
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bioRxiv - Physiology 2021Quote: ... Mouse ear biopsies were taken and digested in DirectPCR lysis reagent (Viagen Biotech, 102-T) with proteinase K (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA from clones was isolated using DirectPCR (Tail) reagent (Viagen, catalog no. 102-T) and Vcan exon 2 and exon 3 were amplified using Phusion Taq (NEB ...
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bioRxiv - Cancer Biology 2022Quote: Zebrafish were genotyped using DNA extracted from fin clipped tissue using DirectPCR lysis reagent (Viagen) complemented with 0.1 mg ml-1 proteinase K ...
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bioRxiv - Neuroscience 2023Quote: ... Cells pellets were lysed and gDNA extracted using DirectPCR lysis reagent CELL (Viagen, 301-C) and Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102-T), 1x Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102- T), 1× Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Genetics 2022Quote: ... Clipped fin tissue was digested in 30 μl DirectPCR Lysis Reagent (Mouse Tail) (Viagen, 102-T) with 40 μg/ml Proteinase K (Invitrogen ...
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bioRxiv - Immunology 2019Quote: Sorted CD4+ and CD8+ T cells were lysed in DirectPCR Lysis Reagent (mouse tail) (Viagen Biotech) with 1mg/ml proteinase K (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... ears of mice were clipped and genomic DNA was extracted using DirectPCR Lysis Reagent (Viagen Biotech). PCR amplification was performed using GoTaq Green Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted using 30 μL of DirectPCR lysis reagent (Viagen Biotech, Los Angeles, CA) mixed with 30 μL of water containing 12 μg of proteinase K ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was obtained by lysing ear punches in DirectPCR Ear Lysis Reagent (Viagen, 102-T) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... PCR using genomic DNA extracted from embryonic sacs digested with DirectPCR Lysis Reagent (Viagen, 301-C) containing Proteinase K (final concentration 6 U/mL ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse tail was treated with 200 μl of DirectPCR Lysis reagent (Viagen Biotech, CA, USA) with 0.5 mg/ml of Proteinase K (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Genotyping was done as follows: Genomic DNA was extracted from tail biopsies using PCR reagent (Viagen) supplemented with Proteinase K (1 mg/mL) ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from ear punches was extracted by incubating samples with DirectPCR Lysis Reagent (Viagen 102-T) and Proteinase K at 55°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Adherent cells were washed twice with Dulbecco’s PBS (-) (D-PBS (-)) and treated with DirectPCR Lysis Reagent (Viagen: 0.5X DirectPCR Lysis Reagent ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted after lysing cells with 200μl of DirectPCR lysis reagent (Viagen biotech, 102-T) for 15min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...