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Citations for Viagen Biotech :
51 - 70 of 70 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Mice were routinely genotyped using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5□mg/mL proteinase K (Worthington) ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 180 μL of DirectPCR (Cell) Lysis Buffer (Catalog No. 301-C, Viagen Biotech, St. Louis, MO) and 20 μL of Proteinase K (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech). 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher ...
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bioRxiv - Genetics 2021Quote: Crude lysates for conventional PCR genotyping were prepared from ear biopsies or yolk sacs using DirectPCR Lysis Reagent (Viagen Biotech). Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix ...
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bioRxiv - Cell Biology 2022Quote: Mice were genotyped by NGS using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5 mg/mL proteinase K (Worthington) ...
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bioRxiv - Developmental Biology 2024Quote: ... Established colonies were manually picked and expanded for screening indel mutations using PCR amplification of a region spanning the targeted gRNA region (genomic DNA isolated using DirectPCR Lysis Reagent (Tail) (VIAGEN), primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Editing control wells were harvested and cells were lysed with 30 μl DirectPCR lysis buffer (Viagen cat. no. 301-C) supplemented with 1 mg/ml proteinase K (Bioline ...
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bioRxiv - Genetics 2023Quote: ... as single cells into 96-well plates to establish clonal populations and subsequently genotyped for deletions using DirectPCR Cell Lysis buffer (Viagen), and cells positive for deletions were expanded (Fig ...
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bioRxiv - Cancer Biology 2022Quote: Total DNA was extracted from ear-clips of adult mice or tails of day (E) 13.5 embryos using tail lysis buffer (Viagen Biotech) and proteinase K ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated by enzymatic digestion of tail clips using Direct-PCR Lysis Reagent (Viagen Biotech, Los Angeles, CA, USA). Genotypes were determined by performing PCR amplification of genomic DNA using allele-specific primers ...
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bioRxiv - Genomics 2022Quote: ... followed by the addition of 180 μL of DirectPCR (Cell) Lysis Buffer (Catalog No. 301-C, Viagen Biotech, St. Louis, MO) and 20 μL of Proteinase K (Catalog No ...
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bioRxiv - Microbiology 2023Quote: ... Mouse genotypes were assessed from genomic DNA isolated from ear clips via digestion in DirectPCR Digestion Buffer + Proteinase K (Viagen Biotech) using the following primer pairs ...
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bioRxiv - Developmental Biology 2023Quote: ... or ear clips (mice) were lysed for at least 4 h at 56°C in 50 µl lysis buffer (Viagen Biotech) with 10% proteinase K ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Cell Biology 2020Quote: ... The mice genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotyping of mice was performed by PCR with specific primers provided by the vendors using lysed tail from each mouse [0.3 cm tail in 100 mL of PCR Direct (tail) solution (Viagen Inc., Los Angeles, CA) with 1 mg/mL proteinase K at 55°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were transferred to 96-well plates to be expanded and genotyped by PCR after crude DNA extraction with the DirectPCR Lysis Reagent (Viagen Biotech #302-C). Clones with a correct insert were further verified by Sanger sequencing and Western blot.
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was isolated from a 5 mm tail biopsy using a rapid digestion method where 95 μl of lysis buffer reagent (Viagen Biotech, Los Angeles, CA; Cat: 102-T) and 5 μl of Proteinase K (20 mg/ml ...