Labshake search
Citations for Viagen Biotech :
1 - 50 of 70 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... in Direct PCR Lysis Buffer (Viagen) at 55° C for 2h ...
-
bioRxiv - Cell Biology 2023Quote: ... were lysed in Direct PCR (Tail) lysis buffer (Viagen, Biotech, #102-T) supplemented with 40 µg/mL Proteinase K (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... 20 mg of each tissue were dissolved in direct PCR buffer (VIAGEN) with proteinase K overnight at 65°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Clones were screened by PCR using primers that flank the SUMO4 gene using genomic DNA purified with direct PCR buffer (Viagen). Clones that displayed reduced size of SUMO4 PCR product were sequenced by Sanger sequencing to confirm disruption of the SUMO4 locus ...
-
bioRxiv - Genomics 2023Quote: ... Individual nematode was isolated and washed with 10% bleach for 10 seconds and transferred into with 200µl PCR tube containing lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
-
bioRxiv - Molecular Biology 2024Quote: ... After adding 200 μl of Direct PCR Tail (Lysis buffer) (Viagen, 102-T) and 4 μl of proteinase K (Invitrogen ...
-
Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Mice were genotyped using PCR on cut toes lysed in Direct Tail lysis buffer (Viagen) with primers as indicated in Supplemental Table S2.
-
bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
-
bioRxiv - Developmental Biology 2020Quote: Ear clips or yolk sacs were lysed either overnight (ear clips) or for 2 hours (yolk sacs) at 55°C in 200 μl of Direct PCR tail lysis buffer (Viagen) supplemented with 200 μg/ml Proteinase K (20 mg/ml stock solution) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Genotypes were determined by polymerase chain reaction (PCR) after O/N digestion using DirectPCR (Tail) Lysis buffer (Viagen, Los Angeles, CA) per manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... Mouse genotypes were assessed by PCR from genomic DNA isolated from ear clips via digestion in DirectPCR Digestion Buffer + Proteinase K (Viagen Biotech) using the following primer pairs ...
-
bioRxiv - Cancer Biology 2024Quote: Mouse genotypes were determined by PCR analysis of DNA derived from earclips or embryonic tails using direct tail lysis buffer (Viagen Biotech) and Proteinase K ...
-
bioRxiv - Microbiology 2022Quote: Crude lysates of single worms were produced as follows: 10 μl lysis buffer (100X stock solution: 1000 μl Direct PCR Lysis Reagent (Cell) (Viagen, 301-C), 50 μl 1 M DTT (Invitrogen ...
-
bioRxiv - Physiology 2024Quote: ... Genotyping was performed via PCR from tail clip biopsies after proteinase K digestion in DirectPCR tail lysis buffer (Viagen Biotech 102-T). PCR of genomic DNA was performed using Apex Taq Master Mix and allele-specific primers as previously described73.
-
bioRxiv - Genomics 2022Quote: DNA for genotyping was extracted from tail clips as follows: tail clips were incubated overnight at 55° C in a solution of 200uL digestion/lysis buffer (Viagen Direct PCR (tail), Los Angeles ...
-
bioRxiv - Molecular Biology 2021Quote: ... Pools of 2 or 3 E4.5 blastocysts were lysed in 10 μl lysis buffer (DirectPCR buffer, Viagen, supplemented with 2.5 μg/ml Proteinase K ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA from two-cell stage mouse embryos was extracted by transferring the embryos one hour and six hours after injection into 5 μl lysis buffer (DirectPCR buffer, Viagen, supplemented with 2.5 μg/ml Proteinase K ...
-
bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated using Viagen Direct PCR (Viagen). The presence of oncogenic and silent mutations in exons and insertions or deletions in introns of BRAF ...
-
bioRxiv - Molecular Biology 2019Quote: ... Mice were genotyped using Direct Tail lysis buffer (Viagen) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was isolated using DirectPCR lysis buffer (Viagen). Gene disruption was analysed by PCR using primers spanning the HIF1α 5’ UTR Sense 5’-cagtgctgcctcgtctga– 3’ and Antisense 5’ caatcccattaacgccgagg-3’ ...
-
bioRxiv - Neuroscience 2022Quote: ... Genotyping of Fgd4fl/+ mice was performed by PCR on DNA isolated from tail biopsies using Direct PCR lysis reagent (Viagen Biotech Inc., USA), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in direct PCR reagent (Viagen 301-C) supplemented with 1 μL proteinase-K (Viagen 501-PK) ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were digested in Direct PCR mix (Viagen #102-T) with 1.5% Proteinase K (Quiagen #19133 ...
-
bioRxiv - Immunology 2020Quote: ... sorted populations were lysed in 40 μl lysis buffer (Viagen) containing 0.5 mg/ml Proteinase K (Invitrogen ...
-
bioRxiv - Molecular Biology 2019Quote: gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
-
bioRxiv - Microbiology 2019Quote: ... gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... DNA for PCR was extracted using DirectPCR Lysis Reagent (Viagen Biotech) plus 10 µg/ml proteinase K (Roche) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech) with Proteinase K (O/N 55 °C) ...
-
bioRxiv - Neuroscience 2019Quote: ... DNA extracted from toe biopsies (Lysis buffer and proteinase K, Viagen) was amplified using a Scn1a-specific forward primer (TMF 441-TTCCATCCCAAGGAAATACCATGT ...
-
bioRxiv - Neuroscience 2023Quote: ... ear punches were digested in DirectPCR buffer (Viagen, Cedar Park, TX) with 0.8 mg/ml proteinase K followed by PCR amplification of the resulting DNA ...
-
bioRxiv - Immunology 2024Quote: ... sorted splenic populations were lysed in 40 μL lysis buffer (Viagen) containing 0.5 mg/ml Proteinase K (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... before removing the supernatant and mixing with 25 μl lysis buffer (Viagen), Proteinase K (New England BioLabs ...
-
bioRxiv - Microbiology 2019Quote: ... before removing the supernatant and mixing with 25 μl lysis buffer (Viagen) and Proteinase K (New England BioLabs) ...
-
bioRxiv - Microbiology 2023Quote: ... or DirectPCR™ Lysis Reagent (Direct buffer) (Viagen Biotech Inc., CA, USA), as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissue was lysed by incubation in tail lysis buffer (Viagen, 102-T) with 0.1 mg/ml proteinase K (Diamond ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA of was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Genotyping of mutant embryos was performed after a lysis step (Lysis Buffer (Viagen)) with 1,5% Proteinase K (Qiagen).
-
bioRxiv - Genetics 2022Quote: ... A fraction of the expanded population was lysed in Direct PCR Lysis reagent (Viagen) with proteinase K (Roche) ...
-
bioRxiv - Neuroscience 2021Quote: ... gDNA was extracted overnight at 55°C in Tail Lysis Buffer (Viagen 102-T) with Proteinase K (Roche 03115828001 ...
-
bioRxiv - Immunology 2021Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
-
bioRxiv - Immunology 2023Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
-
bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102-T), 1x Taq buffer (Syd Labs #MB042-EUT) ...
-
bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102- T), 1× Taq buffer (Syd Labs #MB042-EUT) ...
-
bioRxiv - Genetics 2019Quote: ... Lysis buffer was prepared by diluting one part direct-lysis reagent (301-C, Viagen Biotech) in two parts destilled water (e.g ...
-
bioRxiv - Genomics 2023Quote: ... PCR using genomic DNA extracted from embryonic sacs digested with DirectPCR Lysis Reagent (Viagen, 301-C) containing Proteinase K (final concentration 6 U/mL ...
-
bioRxiv - Neuroscience 2023Quote: Genotyping was done as follows: Genomic DNA was extracted from tail biopsies using PCR reagent (Viagen) supplemented with Proteinase K (1 mg/mL) ...
-
bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... PCR products were generated using cell lysate for each cell line as template (DirectPCR lysis reagent, Viagen), and then purified by using QIAquick 96 PCR Purification Kit (Qiagen) ...
-
bioRxiv - Developmental Biology 2023Quote: ... tail or ear clips were taken and incubated with DirectPCR lysis buffer (Viagen Biotech; Cat#102-T) containing 10µg/mL proteinase K (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... Mice were routinely genotyped using genomic DNA extracted from tail biopsies using the Direct PCR Lysis tail reagent (Viagen) supplemented with 5 mg/ml proteinase K (Worthington) ...