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Citations for Becton, Dickinson and Company :
501 - 550 of 683 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Clonal HAP1 MSH2 knockout cells were isolated by sorting single GFP positive cells using a FACS Aria II instrument (BD), and colonies were screened for MSH2 exon 6 frameshift mutations ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type and xylR evolved/ transposon strains were first cultured at 37°C for 24 hours in an anaerobic box (AnaeroPack system, Mitsubishi Gas Chemical Company) with disposable single-use anaerobic sachets (GasPak EZ Anaerobe Container System, BD). Then ...
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bioRxiv - Biochemistry 2021Quote: ... Single cells were sorted in into 96-well plates using an BD FACSMelody cell sorter (BD Biosciences, Franklin Lakes, NJ). Correctly targeted clones were identified by Sanger sequencing.
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bioRxiv - Immunology 2021Quote: ... Single cell analysis was performed by flow cytometry and the histogram-overlay graphed (LSRFortessa I; FlowJo xV0.7 software; BD Biosciences). The mean fluorescence intensity (MFI ...
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bioRxiv - Immunology 2021Quote: ... Single cell analysis was performed using flow cytometry and the histogram-overlay graphed (LSRFortessa I; FlowJo xV0.7 software; BD Biosciences). The MFI ratio between LPS MFI and PBS MFI was calculated.
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bioRxiv - Cell Biology 2021Quote: ... The proportion of GFP-positive single cells also staining positive for H3pS10 was determined on an LSR Fortessa (BD Biosciences) flow cytometer and analyzed with FlowJo software ...
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bioRxiv - Microbiology 2019Quote: ... a clonal TRIM34-KO THP-1 cell line was created through single-cell sorting of Cas9/RNP electroporated pools into 96-well plates to create individual clonal lines (BD FACS Aria II – Fred Hutch Flow Cytometry Core) ...
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bioRxiv - Immunology 2021Quote: Spleen and lymph nodes from C57BL/6 mice were surgically removed and single cells were obtained mechanically using a 70μm cell strainer (BD Falcon). Red blood cells were lysed by ACK lysis buffer (Lonza ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were first incubated for 10 minutes on ice with anti-mouse CD16/32 Fc Block (BD Biosciences), and subsequently stained with fluorophore-conjugated antibodies against surface markers at 4°C for 20 mins ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting dissociated cells were filtered through a 30 µm cell strainer (Sysmex) and single alive cells were sorted by FACS (Becton Dickinson Influx cell sorter with BD FACS Sortware 1.2.0.142 ...
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bioRxiv - Cancer Biology 2022Quote: ... and MDAMB231-NS cells separately with the Sample-Tags from the BD Human Immune Single-Cell Multiplexing Kit (BD Biosciences) as described in the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Single cell capture and cDNA synthesis using the Single Cell Capture and cDNA Synthesis kit (#633731, #633733, #633773) were done following the manufacturer’s protocol (BD Biosciences). Libraries were then prepared using the BD Rhapsody WTA Amplification Kit (#633801 ...
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bioRxiv - Genetics 2022Quote: ... lymph node and thymus of Myce and littermate WT control were harvested into single-cell suspension with red cell lysis and stained with CD19-BUV395 (1D3, BD), TCRβ-PE (H57-597 ...
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bioRxiv - Neuroscience 2022Quote: Single-nucleus sorting was carried out on either a BD FACSAria II SORP or BD FACSAria Fusion instrument (BD Biosciences) using a 130 μm nozzle and BD Diva software v8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Selected bacteria were pure-cultured from single colonies thrice before they were inoculated in Zobell medium (1 g yeast extract (BD), 5 g bacto-peptone (BD) ...
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bioRxiv - Neuroscience 2022Quote: ... Single nucleus sorting was carried out on either a BD FACSAria II SORP or BD FACSAria Fusion instrument (BD Biosciences) using a 130μm nozzle ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2 ...
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bioRxiv - Cell Biology 2023Quote: ... Single cell suspension was filtrated through a 70 μm nylon cell strainers (Falcon, Cat# 352350) and FACS sorted by BD Influx flow cytometer to make sure only one cell was presented in each well of 96-well plate.
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bioRxiv - Cancer Biology 2023Quote: ... single-cell suspensions of BILs (0.5–1×106 cells) were stained with Fixable Viability Stain 780 solution (BD Biosciences, 565388) to discriminate live and dead cells ...
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bioRxiv - Cell Biology 2023Quote: ... Single cell suspension was filtrated through a 70 μm nylon cell strainers (Falcon, Cat# 352350) and FACS sorted by BD Influx flow cytometer to make sure only one cell was presented in each well of 96-well plate.
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bioRxiv - Immunology 2023Quote: ... The resulting single cell suspensions were filtered through a 70µm nylon filter and treated with BD Pharm Lyse lysing solution (BD Biosciences) to eliminate erythrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences, Heidelberg, Germany). Erythrocytes were lysed by using Red blood cell lysis buffer (Mitenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: Around 15,000 CD45lowSiglec-H+ and CD45-O1+ single cells each were sorted per sample using a FACSAria III (BD Biosciences) before being encapsulated into droplets with the Chromium Controller (10x Genomics ...
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
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bioRxiv - Molecular Biology 2023Quote: ... (PR)20 resistant cells were trypsinized and dispensed as single cells in 96 well plates by FACSAriaTM III (BD Biosciences). The cells were subsequently exposed to 10 µM (PR)20 for 2 – 3 weeks ...
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bioRxiv - Immunology 2023Quote: ... Spleens and MLNs were macerated and filtered into a single cell suspension using a 10-mL syringe using 40um cell strainers (BD), followed by incubation with ACK Lysis Buffer (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and single staining and flow cytometer calibration was performed with rainbow calibration particles (BD Biosciences, Le Pont de Claix, France).
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were collected from a single soiled diaper and immediately placed in a tube completely submerged in 10 ml of thioglycolate broth (BD Difco™ Fluid Thioglycollate Medium Catalog # 225650) ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were subjected to single cell sorting into 96-well plates using a FACSAria II (BD Biosciences, Heidelberg, Germany). About two weeks after sorting ...
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bioRxiv - Bioengineering 2024Quote: ... the B cells were subjected to single-cell sorting using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ, USA) and collected into 96-well plates pre-loaded with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Primary data analysis including gating single cells based on SSC and FSC was done with FlowJo version 9 (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... and FL single cell suspensions were prepared and flow cytometry was performed as described17,48 using an Accuri C6 (BD Biosciences) analyzer ...
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bioRxiv - Immunology 2024Quote: ... Whole transcriptome analyses of FACS sorted CD45+ cells were performed using the BD Rhapsody Single Cell Analysis System (BD, Biosciences). Cells were stained with Zombie Aqua live/dead (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed with 0.05% SDS (Himedia GRM205) and plated in multiple dilutions on 7H11 (BD Difco 0344C41) agar plates ...
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bioRxiv - Cell Biology 2020Quote: ... cells expressing GFP were sorted as single cells in 96-well plates using the 488 nm laser on FACSAria IIu (BD Biosciences). Clones were then analyzed for the expression of target BBS proteins by immunoblotting and sequencing of DNA surrounding the sgRNA target site.
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bioRxiv - Developmental Biology 2021Quote: ... Sorting gates were established using unstained (GFP- and PI-) and single channel controls (GFP+/PI-; GFP-/PI+) on a FACSAria IIu cell sorter (BD Biosciences). FACS was performed at the Flow Cytometry Core at the University of Pittsburgh School of Medicine Department of Pediatrics ...
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bioRxiv - Microbiology 2021Quote: ... FRET-based fluorescence of single cells were collected on BD FACS Aria II flow cytometer (BD Biosciences, San Jose, CA, USA) with a 70-μm nozzle ...
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bioRxiv - Cell Biology 2022Quote: ... Homogenate was passed through 40-μm nylon filter to obtain a single-cell suspension and subjected to red blood cell lysis reagent (BD Pharm Lyse, BD Biosciences). Cells were subjected to negative selection using CD45 magnetic microbeads (Miltenyi Biotec # 130-052-301) ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were incubated with TrypLE Express for 5 min at 37 °C to generate single cell suspensions and strained through a 40 μm mesh (BD Biosciences). They were then fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (Biolegend ...
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bioRxiv - Genetics 2019Quote: ... in PBS + 2.5% FBS and viable single cells were sorted and deposited into the MS-5 seeded 96 well plate using the FACSAria II (BD Biosciences). Single cells were cultured for 16-17 days with an addition of 100μl erythro-myeloid differentiation media per well at day 8.
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bioRxiv - Microbiology 2021Quote: ... Single colonies from each recovery plate were picked and used to inoculate a 2ml liquid culture of Nutrient Broth (BD Biosciences). Liquid regrowth cultures (R1 ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cancer cells were washed with ice-cold PBS with 2% FBS and stained with Near-IR Live/Dead (BD Biosciences) on ice for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Skin equivalents were dissociated and single viable parasites were sorted based on their tdTomato and Calcein AM signal with a FACSAria III cytometer (BD Biosciences) into 48-well plates containing 2.6 µl of 1x lysis buffer (Takara ...
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bioRxiv - Genomics 2021Quote: ... and 48h after induction of 2i-release the cells been dissociated into single cells using accutase before flow sorting (BD Influx) into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells expressing relevant amounts of green fluorescence were isolated and collected in a cell sorter (BD Biosciences, FACSAria III 4L).
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bioRxiv - Immunology 2022Quote: Spike-specific memory B cells were stained and single-cell-sorted from PBMCs using a three-laser FACSAria cell sorter (BD Bioscience) by gating on live ...