Labshake search
Citations for Becton, Dickinson and Company :
451 - 500 of 683 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... GFP-positive single cells were isolated and sorted into 96-well plates by FACS Aria cell sorter (BD Biosciences). Cells were maintained in 96-well plates until they reached confluence and then expanded into larger plates for further analysis ...
-
bioRxiv - Pathology 2023Quote: ... single cells were sorted into an eppendorf containing PBS with 5% FCS using the FACSAria III sorter (BD Biosciences).
-
bioRxiv - Systems Biology 2023Quote: ... and Hoechst-positive single nuclei were purified by fluorescent activated cell sorting (FACS) using a FACSAria sorter (BD Biosciences). Nuclei purity and integrity was verified by microscope ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions from tumors or spleens were incubated in Fc block (BD Biosciences, 553142, vol/vol 1/50) at room temperature for 10 min ...
-
bioRxiv - Microbiology 2024Quote: ... Single colonies from sub-streaked plates were frozen down at −80°C in brain heart infusion broth (BHI, BD) containing 15% glycerol for long-term storage.
-
bioRxiv - Immunology 2023Quote: ... and single-cell suspensions were obtained by passage of the lymph nodes through a 70-μm filter (BD Biosciences). The isolated cells were stained with Live/Dead fixable aqua stain (L34957 ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were dissociated into a single-cell suspension and sorted using a fluorescence-activated cell sorting (FACS) machine (BD Biosciences ...
-
bioRxiv - Immunology 2024Quote: ... and mRNA from single cells was captured using Cell Capture Beads according to the manufacturer’s protocol (BD Biosciences, #633731). Targeted cDNA libraries of genes in the BD Rhapsody Immune Response Panel Mm (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... lung single-cell suspensions were stained for flow cytometry using FITC-conjugated anti-Ly-6C (AL-21) (BD Biosciences), PE-conjugated anti-Siglec-F (E50-2440 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stained samples were analyzed using single-color compensation and FMO controls on a Sony SP6800 spectral analyzer and quantified using FlowJo V10.8.1 (BD Biosciences). Cells were gated in the following sequence ...
-
bioRxiv - Systems Biology 2021Quote: ... 4% SDS) using a BD FACSAria Fusion with the Software BD FACSDiva 8.0.1 and 9.0.1 (BD Biosciences), using 488 nm laser and 530/30 filter for the GFP signal and laser 405 nm and 450/50 filter for the Sytox blue.
-
bioRxiv - Biochemistry 2022Quote: ... cells were cultured overnight at 30 °C and 220 r.p.m in 2 ml synthetic drop-out (SD) medium [0.425 g yeast nitrogen base (YNB) (BD Biosciences ...
-
bioRxiv - Molecular Biology 2022Quote: ... Synthetic dextrose minimal medium (SD) contains 6.3 g/L Yeast Nitrogen Base w/o Amino Acids (BD), 2% glucose and 0.1 mg/mL each of required amino acids ...
-
bioRxiv - Microbiology 2022Quote: ... A synthetic defined medium (SD-medium) (yeast nitrogen base without amino acids containing 0.17% ammonium sulfate [BD Difco] ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cellular uptake was detected in the mCherry channel after acquisition of 10,000 single cells per sample using a flow-activated cell sorter (FACS; FACS Aria III, BD Biosciences). Analyses were conducted by FCS Express (De Novo software).
-
bioRxiv - Immunology 2021Quote: ... by negative selection with single-cell splenocyte suspensions by using biotinylated anti-Thy1.2 mAb followed by streptavidin-conjugated microbeads (BD Pharmingen). In brief ...
-
bioRxiv - Developmental Biology 2021Quote: ... and single tdT+ cells were obtained by fluorescence-activated cell sorting (FACS) on a BD Influx Cell sorter (BD Biosciences). Living cells were gated on FSC ...
-
bioRxiv - Developmental Biology 2022Quote: ... about 1×106 single cells for each group were incubated with Alexa 647-labeled CD31 antibody (1:1000 dilution, BD) for 30 min ...
-
bioRxiv - Microbiology 2019Quote: ... baumannii datasets were selected based on the availability of isolates collected during a single survey that were tested for CIP susceptibility and whole genome sequenced using consistent platforms (in both cases, the BD-Phoenix system and either Illumina MiSeq or NextSeq).
-
bioRxiv - Cancer Biology 2019Quote: ... single-copy TurboGFP gene was generated by transducing A549 with pLenti CMV Neo/TurboGFP lentivirus (MOI 0.3) and single cell FACS sorting using a FACSAria (BD Biosciences). A549GFP cells were subsequently transduced with lentiCas9-Blast or pSpCTRE lentivirus (MOI 0.3 ...
-
bioRxiv - Developmental Biology 2020Quote: Single cell suspensions were washed twice with cold PBS and filtered with a 70 µm filter (BD Falcon, Cat# 352350). Prior to fixation ...
-
bioRxiv - Immunology 2021Quote: ... Blood was collected in a single session for all participants via venipuncture into five 10 ml vacutainer K2EDTA blood collection tubes (367525, BD) per donor ...
-
bioRxiv - Neuroscience 2020Quote: ... Single-nucleus sorting was carried out on either a BD FACSAria II SORP or BD FACSAria Fusion instrument (BD Biosciences) using a 130 µm nozzle ...
-
bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...
-
bioRxiv - Biochemistry 2020Quote: ... Induction of the lbpAp promoter by IBs in single cells was monitored over time by changes of the GFP signal using flow cytometry (Instrument: BD FACS-Aria™SORP cell sorter ...
-
bioRxiv - Microbiology 2020Quote: ... Culture supernatants were screened for specificity by ELISA and positive cultures were single cell sorted using a Beckman Coulter Legacy MoFlo MLS High Speed Cell Sorter (BD). Monoclonal cultures were expanded in DMEM (Sigma ...
-
bioRxiv - Neuroscience 2019Quote: ... mice received a single treatment with an antibody against Ly6G (α-Ly6G; IP 4 mg/kg; BD Bioscience No.: 561005) or with an isotype control antibody (Iso-Ctr ...
-
bioRxiv - Cancer Biology 2020Quote: ... in PBS + 2.5% FBS and viable single cells were sorted and deposited directly onto the MS-5 seeded 96-well plates using the FACSAria II (BD). Single cells were cultured for 17 days and 100μl of fresh erythro-myeloid promoting media was added at day 8.
-
bioRxiv - Cancer Biology 2021Quote: ... Frozen samples were thawed under sterile conditions and single cell preparations were incubated with anti-CD16/CD32 antibody (BD Biosciences) for 15 minutes and stained with the indicated antibodies following standard procedures ...
-
bioRxiv - Cell Biology 2021Quote: ... Single cell suspensions for myeloid panel staining were incubated in Fc Block (Cat#553142, BD Biosciences, Franklin Lakes, NJ, USA). Fluorescent minus one staining controls were used for both panels where required ...
-
bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
-
bioRxiv - Genomics 2022Quote: ... Obtained single-cell suspensions were subjected to a BD Rhapsody system with BD Rhapsody Targeted & Abseq Reagent kit (BD Biosciences) following the manufacture’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with FACS buffer and passed through a 70 μM single cell filter prior to acquisition with the Symphony A5 flow cytometer (BD). Flow cytometry settings were defined for each patient with single staining for each antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... We then collected the FBs and single cell sorted in to 96 well plate by flow cytometry (BD Arial II) and generated a clonal FB line expressing the CRISPRi machinery ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were grown for ∼7 days and subcloned by single cell sorting on a BD FACSAria UV instrument (BD Biosciences). Thap1-/- clones were confirmed by Sanger sequencing as described above.
-
bioRxiv - Microbiology 2021Quote: ... After 48h single colonies were picked and inoculated into 15ml falcon tubes containing 3ml nutrient broth (5g/L peptone BD difco ...
-
bioRxiv - Microbiology 2021Quote: ... After 48h single colonies were picked and inoculated into 15ml falcon tubes containing 3ml nutrient broth (5g/L peptone BD difco, BD Bioscience ...
-
bioRxiv - Immunology 2020Quote: ... Single cell suspensions of splenocytes were prepared after the spleens were homogenized through 100μm filter (BD Biosciences, Bedford, MA, USA), lysed with lysis buffer and washed twice with cold PBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were returned to normal media and were single-cell sorted from a mass culture for EGFP+ by BD Influx cell sorter into 96-well plates ...
-
bioRxiv - Physiology 2022Quote: ... EC-specific H2BGFP and tdTomato fluorescences were directly analysed after single / live cell gating in forward scattered / side scattered plot using FACSVerse flow cytometer (BD). FlowJo software (version 10.5.3 ...
-
bioRxiv - Immunology 2022Quote: ... Single cells isolated using Single Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System following the manufacturer’s protocol (BD Biosciences). After priming the nanowell cartridges ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell clones were sorted into a 96-well U-bottom plate via a BD FACSAria flow cytometer (BD Biosciences) at the Center for Regenerative Medicine (CRM ...
-
bioRxiv - Microbiology 2019Quote: PP single-cell suspensions were made by grinding PPs through a 70 µm mesh filter (BD Bioscience, San Jose, CA). Cells were transferred to a 96 well U-bottom plate and Fc receptors on cells were blocked with Rat anti-mouse Fc-Block CD16/CD32-Clone 2.4G2 (BD Pharmigen ...
-
bioRxiv - Immunology 2019Quote: ... The single cell suspension (SCS) was then passed over a 70-µm nylon cell strainer (BD Biosciences, San Diego, CA) to further remove clumps ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... we founded four different population from single cells isolated using cells-sorting flow cytometry (BD FACSAria™ IIu; Biosciences-US). Because Dunaliella salina is haploid ...
-
bioRxiv - Biochemistry 2020Quote: Single-cell fluorescence signals (20,000/sample) were analysed by dual-channel flow cytometry with an LSRFortessa cell analyser (BD Biosciences). GFP fluorescence and Alexa Fluor 488 fluorescence was detected with excitation laser 488 nm and a 530/30 emission filter and Turquoise fluorescence was detected with excitation laser 405 nm and a 450/50 emission filter ...
-
bioRxiv - Immunology 2019Quote: ... Cells were resuspended in FACS buffer and passed through a 70μm single cell filter prior to flow cytometry analysis (LSR fortessa, BD biosciences). The first six donors were used to optimize the staining panel and cytometer settings ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed in FACS buffer and passed through a 70uM single cell filter prior to acquisition with the LSR fortessa (BD). Flow cytometry settings were defined for each patient material using single stainings for each antibody ...
-
bioRxiv - Biochemistry 2019Quote: ... and single cell fluorescent signals (20,000/sample) were analysed by dual-channel flow cytometry with an LSRFortessa cell analyser (BD Biosciences). GFP and mCherry fluorescence was detected with excitation laser 488 nm ...