Labshake search
Citations for Becton, Dickinson and Company :
601 - 650 of 5651 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Antibodies to intracellular proteins were added to samples for 20 minutes at 4°C in the dark and then washed with PermWash (BD). Flow cytometry analysis was performed using a LSRFortessa flow cytometer (BD ...
-
bioRxiv - Neuroscience 2021Quote: ... 75μg of proteins from control and ALS post-mortem mCTX homogenates was incubated with 5μL anti-DRP1 (BD Bioscience, CA), and GAL4 immunoprecipitation buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Full-length open reading frames or truncated forms of the target proteins were inserted into pGADT7 (AD) or pGBKT7 (BD) vectors (Clontech Laboratories) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... between the NdeI or NcoI and BamHI restriction sites in order to express N-terminal fusion proteins with the GAL4 DNA binding domain (BD) or with the GAL4 activation domain (AD) ...
-
bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
-
bioRxiv - Plant Biology 2019Quote: ... The coding sequence of AGL16 was cloned into pAD-GAL4-2.1 (AD vector) and the putative protein binding sites were cloned into pHIS2 (BD vector). These constructs of pAD and pHIS2 plasmids were introduced into Y187 yeast strain by heat shock ...
-
bioRxiv - Biochemistry 2020Quote: ... this threshold is likely a consequence of the fact that the target protein in all six ternary complex crystal structures is a bromodomain (BD), of either Brd4 ...
-
bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
-
bioRxiv - Immunology 2020Quote: ... The levels of IFNg protein expression was determined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
-
bioRxiv - Immunology 2020Quote: ... IFN-γ and IL-4 protein in culture supernatants were quantified using the Cytokine Bead Array according to the manufacturer’s instructions (BD Biosciences)
-
bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
-
bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
-
bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
-
bioRxiv - Synthetic Biology 2022Quote: ... all samples were mixed 1:1 with prewarmed CytoFix Fixation Buffer (BDBiosciences #554655) and incubated at room temperature for 15 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000), rabbit anti-MCM3 (Cell Signaling ...
-
bioRxiv - Developmental Biology 2019Quote: ... Figure 1 and figure 2: CD235a-PeCy7 (1:100, BD, GA-R2(HRI2)) ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Pdcd6ip (AIP-1/Alix) (BD transduction lab 611620, 1:500); goat polyclonal anti-TSG101 (Santa Cruz sc-6037 ...
-
bioRxiv - Microbiology 2020Quote: ... 250 µg mL−1 L-cycloserine and 1.5% weight vol−1 (BD, USA) (TCCFA plates) ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti-nestin (1:200 in vivo, 1:500 in vitro, BD Bioscience), chicken anti-vimentin (1:10,000 ...
-
bioRxiv - Cancer Biology 2022Quote: CD44 (1:200, IM7, #560568), CD11b (1:800, M1/70, #563553) (BD Biosciences); MHC-I (1:100 ...
-
bioRxiv - Physiology 2022Quote: ... Gr-1 (catalog no. 550291, 1/50, BD Pharmingen, Franklin Lakes, NJ, USA) and the cholangiocyte marker cytokeratin-19 CK19 (TROMA-III 1/500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Col IΔ R/R fibroblasts diluted 1:1 with BD Matrigel (BD Biosciences) in a total volume of 100 μl ...
-
bioRxiv - Cancer Biology 2019Quote: ... A 100 μl volume of 1:1 mixture of Matrigel (354248; BD Biosciences) and 1 × 106 MiaPaCa2 cells alone ...
-
bioRxiv - Cancer Biology 2020Quote: ... mouse anti-Aurora kinase 1 (Cat no. 611082, 1:100 PLA, BD Biosciences), mouse anti-Actin (MAB1051 ...
-
bioRxiv - Immunology 2021Quote: ... anti-CD101 (clone Moushi101, 1:100 eBioscience; clone 307707, 1:100 BD Biosciences). For MMP-9 and IL-1ß intracellular staining ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal antibodies to Aurora B (AIM-1; BD Bioscience 611082, 1:100) and H3S10ph (6G3 ...
-
bioRxiv - Cancer Biology 2022Quote: ... or RIVA cells were mixed in a 1:1 in Matrigel (BD Biosciences)/PBS and injected into the flanks of 10-week-old NSG mice ...
-
bioRxiv - Bioengineering 2024Quote: ... and anti-E-cadherin-1 (ECAD; 1:500; 610181, BD Biosciences, CA, USA). Nuclear counterstaining was performed with DAPI (Nacalai Tesque ...
-
bioRxiv - Genomics 2023Quote: ... and incubated overnight with Purified Mouse Anti-TEF-1 (1:500, BD-610922) or mouse anti-YAP1 H-9 (1:150 ...
-
bioRxiv - Immunology 2019Quote: ... were injected subcutaneously along with engineered stromal cells (1:1 to 1:5 ratio HSPC/MS5) in 200 μl of ice-cold Matrigel® (BD Biosciences). Mice were sacrificed at day 12 of differentiation by cervical dislocation and Matrigel® plugs were collected ...
-
bioRxiv - Immunology 2021Quote: ... and re-suspended in 100 μL permeabilisation buffer (FACS buffer, 1% BSA, 1 mM EDTA in PBS containing 1 X BD permeabilisation reagent). FITC-conjugated anti-influenza NP monoclonal antibody (Abcam clone 20921 ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
-
bioRxiv - Cell Biology 2022Quote: ... F4/80 (1:100 dilution, clone Cl:A3-1; Serotec, Cat# MCA497) and CD11b (1:100 dilution, clone M1/70; BD Biosciences, Cat# 550282).
-
bioRxiv - Immunology 2023Quote: ... FITC CD8 (Clone 53-6.7, 1:25), Alexa Fluor 700 CD44 (Clone IM7, 1:50), and PE CD62L (Clone MEL-14, 1:50) (BD Pharmingen, CA), and the yellow LIVE/DEAD® dye (1:300) ...
-
bioRxiv - Cancer Biology 2023Quote: ... SCC13 tumour cells and fibroblasts were mixed in a 1:1 ratio in complete DMEM containing 1% Matrigel (BD Biosciences, Cat# 354230). Cells were plated on 8-well glass bottom culture slides (Corning ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100, Anti-GM130 BD Transduction 610823) for 1 hour and 30 minutes at room temperature in blocking/permeabilization solution ...
-
bioRxiv - Immunology 2024Quote: ... labelled K562 at a ratio of 1:1 for 1 hour and conjugate formation was evaluated by flow cytometry (Fortessa X20™, BD Biosciences).
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg ...
-
bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
-
bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
-
bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... macrophage inflammatory protein (MIP-1α) and tumor necrosis factor (TNF-α) were detected using Cytometric Bead Array (CBA) technology (BD Biosciences) and assayed on an Accuri C6 flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2019Quote: Cytometric bead array (CBA) was performed using the mouse soluble protein master buffer kit combined with the appropriate CBA flex sets (BD Biosciences), per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... Green fluorescent protein (GFP) positive cells in HRasL61 with GFP transfected cells were sorted using flow-cytometry (FACS Aria II; BD Bioscience).
-
bioRxiv - Physiology 2022Quote: The geometric mean fluorescence intensities (MFI) corresponding to the probes/target protein levels were determined by flow cytometry acquired on Aria II (BD Biosciences) or CytoFLEX (Beckman Coulter ...