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Citations for Becton, Dickinson and Company :
551 - 600 of 765 citations for Rcombinant Cynomolgus PDCD1LG2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 15 min at 4°C with Fc-block (anti-CD16/CD32, 2.4G2, BD biosciences) before staining ...
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bioRxiv - Cell Biology 2021Quote: ... and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences, USA). Samples were analyzed by Guava® easyCyte™ Flow Cytometer (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: The following anti-mouse antibodies were used following manufacturer’s instructions: anti-CD16/CD32 Fc blocking antibody (BD Biosciences), anti-CD24-FITC (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in FC stain buffer prior to data collection via an LSR Fortessa flow cytometer (BD). Analysis was done using FlowJo software (version 10.7.1) ...
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bioRxiv - Immunology 2022Quote: For analysis of various thymus stromal cell populations human samples were blocked with human Fc-block (BD Bioscience) for 10 minutes at 4°C and then stained with Lineage cocktail-FITC ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked with 100 mL solution containing anti-mouse CD16/CD32 (1:100, BD Pharmingen, 553141) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2023Quote: ... Aspecific epitope binding was blocked with 0.5 mg/mL human Fc Block (BD Biosciences, San Jose, CA, USA). Mononuclear cells were stained for extracellular epitopes for 1h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were Fc-blocked with anti-mouse CD16/CD32 (1:100, Cat# 553141, BD Biosciences, San Jose, CA) followed by surface marker staining with antibodies to MerTK (2B10C42 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with FACS buffer (PBS + 0.1% BSA) and incubated with Human Fc block (5µl per 2×106 cells; BD) at room temperature for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 15 mins) and resuspended in 3mL DPBS supplemented with 30 µL Human BD Fc Block (BD Pharmingen, 564220). The sample was gently rocked at room temperature for 30 mins ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and suspended in 50 μL of FCM buffer (0.5% BSA and 0.05% sodium azide in PBS) with 2.5-10 μg of Human BD Fc Block (BD). Cells were stained using the antibodies listed in Supplementary Table 1 for 15 minutes in the dark at room temperature and washed three times with FCM buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were from BD Pharmingen and Santa Cruz Biotechnology Inc. ...
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bioRxiv - Immunology 2022Quote: Flow cytometry of peritoneal cells was performed using anti-mouse CD16/32 (Fc Block, BD Biosciences, San Jose, CA) before staining with primary antibody or isotype controls ...
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bioRxiv - Immunology 2019Quote: ... The cells were resuspended in FACS buffer (HBSS/ 1% FBS) then treated with Fc block (anti-mouse clone 2.4G2 and anti-human clone 3G8) purchased from BD and stained with specific antibodies ...
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bioRxiv - Microbiology 2021Quote: ... 1×106 cells were blocked with mouse BD FC block™ (5 μg/mL, BD Pharmingen, Cat No. 553141) and then stained using fluorescent-labeled monoclonal antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... for 15 minutes to block Fc receptors and then stained using with antibodies to CD45-APC-Cy7 (BD 557659) and SYTOX Red Dead Cell Stain (Life Technologies S34859 ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in perm/wash solution and resuspended in 0.5% PFA-FC stain buffer prior to running on a Symphony A5 flow cytometer (BD). Analysis was performed using FlowJo software ...
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bioRxiv - Systems Biology 2022Quote: Citrated blood collected from infected and control mice was diluted with HEPES buffer containing mouse Fc-block (BD Pharmingen). Platelets ...
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bioRxiv - Immunology 2019Quote: ... FCS in PBS and were stained for 1 hour at 37°C with Vγ5 TCR-FITC (clone 536; BD), MHCII-AF647 (I-A/IE ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IgG1 Fc control and anti-human HLA class II (anti-DQ, -DR-DP) (clone Tu39, BD Biosciences) were also used at 2.5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... and 10% (v/v) FCS (from herein referred to as DC culture media. The antibodies were purchased from BD, Miltenyi ...
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bioRxiv - Cancer Biology 2021Quote: ... dissociated tumor cells were Fc receptor blocked by incubating 1*106 cells/50-100 µl of staining buffer with 2.5 µg of Human Fc Block (Cat # 564220; BD) for 10 minutes.
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:100, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed and blocked with blocking buffer plus Fc receptor blocking antibody (Clone Fc1.3216, BD Biosciences, United Kingdom). Cells were incubated at RT for 10 min then centrifuged (800 g ...
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bioRxiv - Immunology 2022Quote: Cells were incubated with purified anti-mouse CD16/CD32 clone 2.4G2 (Fc block; BD Biosciences, San Jose, CA, USA), and stained as described with H-2k(d ...
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bioRxiv - Cell Biology 2023Quote: ... Data collection was done using BD FACSDiva™ software and .fcs files were formally analyzed with FlowJo (FlowJo, BD). All flow cytometry experiments contained single color controls for compensation and gating.
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:200, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... All data were converted to FCS 3.0 using NF Profession (Version 1.0, NanoFCM) and analyzed with FlowJo (Version 10.8.1, BD, Franklin Lakes, NJ).
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bioRxiv - Pathology 2023Quote: ... single cells were sorted into an eppendorf containing PBS with 5% FCS using the FACSAria III sorter (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Cells were washed in PBS+2% FCS and permeabilised for 10 minutes in 70ul of Cytofix/Cytoperm (BD Biosciences). After washing in 2mL of PermWash ...
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bioRxiv - Microbiology 2023Quote: ... Fc receptor blockade and viability staining were simultaneously performed using Rat anti-mouse FcψRIII/II (CD16/32; BD Biosciences) and ZombieNIR (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from tumors or spleens were incubated in Fc block (BD Biosciences, 553142, vol/vol 1/50) at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... 1% FCS and 2mM EDTA) and stained for extracellular molecules with the following antibodies: anti-CD8a (53-6.7, BD), anti-CD4 (GK1.4 ...
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bioRxiv - Immunology 2024Quote: ... and Fc-receptors were blocked (2.4G2) before staining with monoclonal antibodies before collection on a Fortessa flow cytometer (BD). Fluorochrome-conjugated antibodies to the following cell surface molecules were used ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... with all differentiations performed in biological replicates. Samples were tagged or “cell hashed” (Stoeckius et al. 2018) with distinct BD sample tags (BD Bioscience, cat#PN 633780) to enable demultiplexing of cells ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...