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Citations for Becton, Dickinson and Company :
451 - 500 of 765 citations for Rcombinant Cynomolgus PDCD1LG2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with Fc block solution (purified anti-mouse CD16/CD32, clone 2.4G2, BD Biosciences) for 15 min at room temperature to prevent non-specific binding ...
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bioRxiv - Cancer Biology 2020Quote: ... RBCs were lysed and samples were subsequently incubated with anti-mouse Fc block (BD Pharmingen, 553142) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with Fc block (1:500 dilution, Cat. # 553142, clone 2.4G2, BD Biosciences) diluted in FACS buffer for 5 minutes to block nonspecific binding of antibodies to cellular Fc receptors ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes followed by an Fc block (catalog number 553142, BD Pharmingen, Franklin Lakes, NJ) at 1:1,000 for 15 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Flow Cytometry Standard (FCS) 3.0 files were analyzed with FlowJo software version X 0.7 (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Barcoded cells were then combined into a single tube prior to Fc receptor blocking (BD Biosciences) and staining with a cocktail of metal-conjugated antibodies against cell-surface markers for 30 minutes at RT with frequent gentle shaking ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were then stained with directly labeled antibody against CD5 (APC ...
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bioRxiv - Immunology 2022Quote: Cells for B cell staining panels were resuspended in 50uL Human Fc-Block (BD Cat#564220) diluted to1:500 in PBS + 1%FBS and incubated for 30 minutes at 4°C prior to washing and surface staining ...
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bioRxiv - Immunology 2022Quote: Splenocyte cell suspensions (~2 million cells in HBSS) were incubated with Fc block (553142, BD Biosciences) for 10 min on ice followed by the direct addition of the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Final gating analysis was conducted using FCS Express software (De Novo) or FlowJo V10.8.1 (BD Biosciences). Control wells containing no chemokine in the bottom well were averaged and normalized as 100% migration ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked with rat anti-mouse CD16/CD32 Fc block (2.4G2, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions of BAL and lung digests were preincubated with Fc Block antibody (BD PharMingen) in PBS + 2% heat-inactivated FBS for 10 min at room temperature before staining ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS at 37 °C with either PMA/ionomycin (Leukocyte activation cocktail, BD Biosciences) for 3 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked with anti-FcR (anti-CD16 CD-32 at 5μg/ml, BD Pharmingen). After 2 washes in PBS 2% FCS ...
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bioRxiv - Immunology 2021Quote: ... and with Mouse BD Fc Block™ (≤ 1 μg/million cells in 100 μl, BD Biosciences) at 4°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block, BD Biosciences) antibody for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Myelin-depleted cell suspensions were incubated with an anti-CD16/CD32 Fc-blocking antibody (BD Biosciences) for 30 minutes at 4 °C to avoid Fc receptor binding and then washed once with FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked in FACS buffer supplemented with anti-CD16/CD32 antibody (Fc Block, BD Pharmingen).
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... and cells were blocked in FACS buffer containing Fc Block anti-CD16/32 (2.4G2) (BD Biosciences), 5% normal hamster serum ...
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bioRxiv - Immunology 2021Quote: ... Unspecific binding by the Fc receptor was prevented by incubation of anti-mouse CD16+CD32 (BD) for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... resuspended in Fc Block (clone 2.4G2, Becton, Dickinson, and Company [BD, Franklin Lakes, NJ], cat. 553142) and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD4 (#560181), CD11b (#553312), 2.4G2 (Fc block, #553142) and Perm/Wash Buffer (#554723) were from BD Pharmingen (San Diego ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down and resuspended in 50μL of human Fc block (BD Biosciences Cat. 564220) in DPBS and incubated for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... samples were blocked with Human BD Fc Block™ solution (1 µL/200,000 cells) (BD Biosciences) for 10 minutes at room temperature to reduce any non-specific antibody staining by Fc Receptors expressed on macrophages ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated in a blocking solution containing Human BD Fc Block (564220; BD Biosciences) diluted 1:25 (v/v ...
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bioRxiv - Cancer Biology 2022Quote: Immune cells were first incubated with Fc block (anti-CD16/CD32 1:100; BD Biosciences #553142) and then stained with one of two immunophenotyping panels diluted in 1x FACS buffer (2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pre-incubated with mouse Fc blocker (BD 553142, 1 μg/million cells in 100μl) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with appropriate antibodies for 25 minutes on ice in the dark ...
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bioRxiv - Cancer Biology 2023Quote: Anti-CD16/CD32 Rat anti-mouse Fc Block (BD Pharmingen, #553142, 0.5 mg/mL, 1:400)
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bioRxiv - Neuroscience 2023Quote: ... and blocked for 15 minutes with 1:20 human FC block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Cell Biology 2024Quote: Freshly thawed human and rhesus CD34+ cells were incubated with human Fc BlockTM (Becton Dickinson (BD), Franklin Lakes ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with fluorescent dye-labeled antibodies after incubation with Fc block reagent (BD Biosciences), and analyzed on the FACSCanto Ⅱ (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: MLN cell suspensions were incubated with Fc-block (anti-CD16/CD32, BD Biosciences, cat no. 553142) followed by staining with antibodies against surface and intranuclear markers ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells stably expressing YFP-tagged BBSome subunits were sorted using the 488 nm laser on FACSAria IIu (BD Biosciences).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
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bioRxiv - Cell Biology 2021Quote: Expression of the various GPR116 constructs tagged with V5 or FLAG was verified by immunocytochemistry (Cytofix/Cytoperm, BD 51-2091KZ). Cells were plated in 8-chamber slides (Ibidi ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... left 24 hours before addition of drug and cell death was assessed following live cell staining with FITC-tagged AnnexinV (BD Biosciences) and addition of PI (Sigma ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II, 70 uM nozzle, BD Biosciences) and brought to a concentration of 1000 nuclei/uL ...
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bioRxiv - Immunology 2021Quote: The PBMCs were first resuspended in FACS buffer (PBS + 2% FBS) containing Fc blocking antibodies (BD, 564765) and incubated for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... cells were spun down and resuspended in 50 μL of human Fc block (BD Biosciences Cat. 564220) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2021Quote: ... After pre-incubation of samples with purified rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block) (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated for 10 min with rat anti-mouse CD16/CD32 Fc Block™ (BD Pharmingen) in Stain Buffer (BD Pharmingen ...