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Citations for Becton, Dickinson and Company :
401 - 441 of 441 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Human cytokines expression (hTNFα and hIFNγ) in human T cells were analyzed using BD LSRFortessa™ cell analyzer - flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (Dako).
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bioRxiv - Immunology 2020Quote: ... High-dimensional analysis by Fast Fourier Transform-accelerated Interpolation-based t-distributed stochastic neighbor embedding (Fit-SNE) was performed using FlowJo (BD, Version 10.6.1). Heatmaps were generated using a web-enabled tool (Heatmapper 14) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were allowed to dry and then hydrated and blocked for 1h at RT with 5%milk/TBS-T and incubated overnight at 4ºC with the primary antibody mouse monoclonal anti-α-Syn 1:1000 (BD Biosciences, 610787) in 5%milk/TBS-T ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified—10 cycles for resting cells and 9 cycles for in vitro-stimulated cells using the BD Rhapsody™ T Cell Expression Panel (BD Biosciences) combined with a supplementary panel developed in-house targeting a total of 1,066 different genes (table S4) ...
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bioRxiv - Immunology 2022Quote: ... cells were spun at 500xG and resuspended in Flt3L DC Buffer (T-cell buffer + 10% B16-Flt3L conditioned RP10G medium + 1000U/mL Mouse GM-CSF [BD Biosciences, #554586]). Dendritic cells were plated in a total volume of 75 μL and rested overnight ...
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... and naïve (CD45+Lin-CD3+CD4-CD8+CD62L+CD69-) and activated (CD45+Lin-CD3+CD4-CD8+CD69+) CD8+ T cells were analysed with LSRFortessa X-20 (BD Biosciences, USA) based on the gating strategy in Fig ...
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bioRxiv - Immunology 2023Quote: ... T cells and RP KD tumor cells were then co-cultured for 6 hours in the presence of GolgiPlug (BD Biosciences 555029) and GolgiStop (BD Biosciences 554724 ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Neuroscience 2020Quote: EAE was induced in 7-week-old female C57BL/6J mice by subcutaneous flank administration of 200 μg MOG35-55 peptide emulsified with complete Freund’s adjuvant (CFA) (MOG35-55/CFA) (BD Biosciences, San Jose, CA, USA) supplemented with inactive ...
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Immunology 2019Quote: ... counted cells and stained with directly conjugated antibodies for T cell markers (CD4-Pacific Blue, CD8-AlexaFluor® 488, CD62L-PE, CD44-AlexaFluor® 647) in the presence of FcBlock (BD Biosciences). We also stimulated 106 splenocytes per well with custom synthesized HBV peptides (Genscript ...
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bioRxiv - Microbiology 2020Quote: ... and end (F1, t = h when cells reached stationary phase) of the competition experiment using an LSRII flow cytometer (BD, Franklin Lakes, NJ). mCherry was excited at 561 nm and measured at 620/40 nm ...
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bioRxiv - Immunology 2022Quote: ... 20,000 BMDMs were plated in a 96 well plate with 50,000 purified naive OT-I T cells labeled with Violet Proliferation Dye 450 (BD Biosciences, Franklin Lakes, NJ). Following 72 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... T cell activation was measured with a PE-conjugated anti-hCD69 on CD3+ gated viable cells by flow cytometry (BD LSRFortessa, BD Biosciences). For T cell proliferation ...
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bioRxiv - Microbiology 2021Quote: Cryopreserved PBMCs were thawed and CD4 T cell enrichment was carried out with negative magnet-activated cell sorting (Beckton Dickinson, BD IMag™, #557939). CD4 T cells were stained with the following monoclonal antibodies ...
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bioRxiv - Microbiology 2020Quote: ... The flow cytometer was operated with Milli-Q water (MerckMillipore, Belgium) as sheath fluid and instrument performance was verified daily using CS&T RUO Beads (BD Biosciences, Erembodegem, Belgium). For the feed samples a modified protocol was used (see Supplementary Experimental Procedures).
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Immunology 2021Quote: Absolute numbers of B cells and T cells in peripheral blood were measured using BD Multitest™ 6-color TBNK reagents and BD Trucount™ Tubes (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Bioengineering 2024Quote: ... the level of CFSE in proliferating T cells was assessed using the BD Accuri™ C6 Plus Personal Flow Cytometer (BD Biosciences, USA).
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD4+ and CD8+ T cells from PBMCs were sorted with the strategies above and the addition of CCR7+CD45RA+ gates (BD 562381 and Biolegend 304134).
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Proliferation of CPD-labeled T cells measured by dilution of the CPD signal and EGFP expression were analyzed using a FACSVerse system (BD Biosciences, Franklin Lakes, NJ) and FlowJo software (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... CD8+ and CD4+ T cells from the tumor and CD8+ CD44hi and CD4+ CD44hi T cells from the dLN were sorted using FACS Aria II or Aria III (BD Biosciences, San Jose, CA). Nonviable cells were excluded from the sorting gate based on forward and side scatter profiles and propidium iodide staining ...
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bioRxiv - Immunology 2020Quote: ... the transduced T cells were resuspended in 200 μl of FACS buffer and tetramer-positive T cells were sorted at the UW Department of Immunology Flow Cytometry Core using a FACS Aria II (BD Biosciences, San Jose, CA) cell sorter equipped with blue (488 nm) ...
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bioRxiv - Immunology 2023Quote: ... and sorted for memory CD4+ T cells (CD4+CD44hiCD62Llo) that were either GFP+ (IL-10+) or GFP- (IL-10-) by a FACSAria flow sorter (BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2023Quote: CD8+ and CD4+ T-cells isolated from spleens of naïve mice using BD-IMAG anti-mouse CD4+ and CD8+ magnetic particles (BD Biosciences, San Jose, CA, USA) were cultured in 96-well plates (Nunc ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Bioengineering 2021Quote: ... The performance of the FACSVerse was verified by the FACSuite software performance quality check using CS&T research beads (BD Biosciences, San Jose, CA, USA). The raw flow cytometry data were exported in FCS format and imported into R (v3.6.1 ...
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bioRxiv - Immunology 2020Quote: ... The purified CD4+ T cells were seeded at 1 × 105 cells/well in the anti-CD3 monoclonal antibody (mAb) (10 μg/mL, BD Pharmingen, Franklin Lakes, NJ, USA) precoated 96-well tissue culture-treated plate ...
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Fixed/ permeabilized cells were incubated for 30 min on ice with: Alexa Fluor® 647-conjugated mouse anti-mouse T-bet (4B10; BD Biosciences cat no. 561264), PE-conjugated rat anti-mouse GATA3 (TWAJ ...