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Citations for Becton, Dickinson and Company :
201 - 250 of 441 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... HH7-2tg-FoxP3hCD2 T cells were incubated with Golgi-Plug and Golgi-Stop (1:100, BD Biosciences) for 4h at 37°C.
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bioRxiv - Immunology 2023Quote: RBD-specific T cell responses were investigated using the mouse IFN-γ ELISPOT Set (551083; BD Biosciences), as per manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were activated with 3 μg/ml plate-bound anti-CD3 (BD Biosciences, USA, 555329) in the presence of different concentrations of glucose (2.8 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell phenotypes and T cell degranulation were measured by flow cytometry (Accuri C6Plus, Canto II, Symphony, BD). Cytolytic activity was performed by 51Cr assay in 2D models and by videomicroscopy in 3D models using an Incucyte® (Sartorius ...
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bioRxiv - Immunology 2024Quote: Dimensionality reduction was performed using the DownSample and t-SNE Plugins in FlowJo™ v10.8.1 (BD Biosciences). For the generation of t-SNE plots ...
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bioRxiv - Immunology 2024Quote: ... Passenger T cells were identified as CD4+ or CD8+ (BUV805 or BV605, clone 53-6.7, BD Biosciences) and HLA-A2+ (PE ...
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bioRxiv - Bioengineering 2023Quote: ... Flow cytometry of stained T lymphocytes was then performed using a BD LSRFortessa Cell Analyzer (BD Biosciences) and the data were analyzed using FlowJo v10 (BD) ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of Leuko Act Cktl with GolgiPlug (BD) plus 50 IU/mL IL-2 ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies for flow cytometry were: rat anti-C-peptide-Alexa Fluor 647 (1:200, 565831, BD Pharmigen) and mouse anti-Glucagon-BV421 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of GolgiPlug and GolgiStop (BD Cat#550583) plus 50 IU/mL IL-2 ...
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bioRxiv - Genomics 2020Quote: ... and PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13-11, BD Biosciences, San Jose, CA, USA), and analyzed on a BD LSRFortessa Cell Analyzer together with negative control samples of iPSCs ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve CD8+ T cells from C57BL/6 splenocytes were sorted using anti-mouse CD8a particles (BD Biosciences, 551516) and cultured under CD3 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells (CD45RA+CD45RO-) were isolated by FACS with a FACSAria III Cell Sorter (BD Biosciences). Naïve CD8+ T cells were activated for two days with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... To assess intracellular cytokine levels T cells were similarly stimulated in the presence of Brefeldin A (BD GolgiPlug) and surface-stained with CD3 BUV395 (BD clone SK7) ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ CD44high T cells from dLN were sorted using FACS Aria II or Aria III (BD Biosciences). Nonviable cells were excluded from the analysis based on forward and side scatter profiles and propidium iodide staining ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T-cells were stained with rat anti-mouse CD8α PE-conjugated antibody (Clone 53-6.7, BD biosciences) and Notch1 with rabbit anti-Notch1 (D6F11 ...
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bioRxiv - Microbiology 2020Quote: ... T lymphocyte staining was performed using CD3 clone 17A2 conjugated with fluorescein isothiocyanate (FITC) (1:200; BD Biosciences), CD4 clone RM4-5 conjugated with phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: Viability of γδ T cells was assessed using the FITC Annexin V Apoptosis Detection Kit I (BD Bioscience).
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bioRxiv - Immunology 2019Quote: ... Absolute numbers of ova-specific CD8+ T cells were calculated from FACS analysis using fluorescing beads (BD Biosciences). All stained cells were analysed on an LSRII or a FACSFortessa (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... The T cells were isolated from the BM aspirates and sorted using the BD FACSAria IIITM (BD Biosciences) with anti-CD4 antibodies (BD Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... Effector function of CD8+ T cells was assessed on day 6 on a Fortessa flow cytometer (BD Biosciences) and quantified according to the CFSE peaks with FlowJo (Tree Star)29 ...
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bioRxiv - Immunology 2021Quote: ... and the universal T cell surface marker CD3 V450 (Mouse Anti-Human CD3, BD Biosciences; catalogue number: 560365) was used at a concentration of 5μL in a 50µL final staining volume per section ...
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bioRxiv - Immunology 2020Quote: Samples were acquired on a BD LSRII (core facility instrument QC-ed daily with BD CS&T beads); PMT voltages normalized per run to pre-determined/optimized ‘Peak-6’ (Spherotech ...
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bioRxiv - Immunology 2022Quote: ... Murine OT-I CD8+ T cells of ex vivo assays were stained with: aCD8a-PE (BD Biosciences, 553033), aCD8a-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... The proliferation of T effector cells was determined at day 4 on a BD Fortessa instrument (BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... T-cells were then stained with CD4 and CDRB and sorted on a FACS Aria Fusion (BD biosciences). CD4+ CDRBhi cells were collected and administered to TCRb-/- or TCRb-/-;Clec12a-/- mice intra-peritoneally (I.P.) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 and CD8 T cell depletion were confirmed using flow cytometry on the BD FACS CantoII (BD Biosciences) from the mouse spleen ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were then isolated by positive selection using magnetic beads coated with CD8 antibodies (BD, USA) and placed into the Transwell lower chamber in the presence of CD28 antibody (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then evaluated stained with fluorescently labeled antibodies recognizing T cell activation markers: CD69-BV711 (BD Biosciences) and CD107a-APC (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... while T cells were identified using CD4hi or CD8hi with the following antibodies: BUV395 CD90.2 - 565257 (BD, USA), BV711 CD4 - 100447 (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... Live CD11b−F4/80−B220−CD90.2+CD4+CD44hi CD4 T cells were sorted using FACSAria III (BD Biosciences), partitioned using a Chromium Controller (10x Genomics) ...
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bioRxiv - Immunology 2023Quote: ... CFSE fluorescence intensity was measured in CD3+CD4+DAPI- T cells with BD FACS CANTO II (BD Biosciences) to measure the proliferation of CD4+CD25- effector T cells with or without the presence of CD8+CD45RClow/-TNFR2+ Tregs or total CD8+CD45RClow/- Tregs.
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bioRxiv - Microbiology 2023Quote: ... followed by intracellular staining with Alexa Fluor 647– conjugated mouse anti-mouse T-bet (clone 4B10; BD Biosciences), PE-conjugated rat anti-mouse GATA3 (clone TWAJ ...
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bioRxiv - Systems Biology 2023Quote: ... Naïve T cells were activated with plate bound anti-CD3ε (5 µg/ml, clone: 145-2C11, BD Biosciences) and soluble anti-CD28 (0.5 µg/ml ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Immunology 2021Quote: ... no stimulation and a peptide pool spanning the spike protein at a concentration of 1 μg ml−1 of each peptide in the presence of 1 μg ml−1 of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Immunology 2021Quote: ... live cells were separated by density gradient and then restimulated by incubation with irradiated APCs from C57BL/6 mice that had been pre-loaded with indicated concentrations of SIINFEKL peptide or by PMA (10ng/ml) and ionomycin (1μg/ml) in the presence of GolgiPlug (BD Biosciences) for 4 hours.
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... aged 8 weeks received subcutaneous injections to both flanks and base of the tail of MOG35-55 peptide (125μg total, Mimotopes) emulsified 1:1 (vol/vol) in Freund’s complete adjuvant (BD Cat# 263810) containing additional Mycobacterium tuberculosis (5mg/mL ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Spleen and lung cells that were stimulated overnight with peptide pools (as described under ex-vivo stimulation and intracellular staining) were fixed and permeabilized with Cytofix/Perm solution (BD) and then stained for intracellular cytokines ...
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bioRxiv - Immunology 2021Quote: ... and I53-50B components of the NP-scaffold (1.2 µg/ml of each peptide) in the presence of 1 µg/ml of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Microbiology 2022Quote: EAE was induced by injecting 8 to 10-week-old female C57BL/6J mice with 150 mg MOG35-55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse subcutaneously in the flanks ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were stimulated with specific or irrelevant peptides at 5μg/mL (JPT Technologies) in the presence of Golgi Plug (BD Biosciences) in complete medium (RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Biochemistry 2023Quote: The coding sequences of SAP05 or SAP05 mutants excluding signal peptides were amplified and ligated into Gateway vector pDEST-GBKT7 (BD). Full length sequences of A ...
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bioRxiv - Microbiology 2023Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Immunology 2024Quote: Splentocyte samples were either aliquoted in mFACS Buffer for staining or resuspended in mR10 and stimulated ex vivo with LCMV peptide DbGP33 (0.2 μg/mL) in the presence of Golgi Plug and Golgi Stop (BD Bioscience) for 5 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: Lymphocytes isolated from the spleen or siIEL compartment of naïve QFLTg_WT or OT-1_WT chimera mice were cultured in vitro with 2µM of FL9 or SL8 peptide respectively together with GolgiPlug (BD Biosciences) for 4.5h ...