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Citations for Becton, Dickinson and Company :
301 - 350 of 354 citations for Peptide B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% bovine serum antigen) and stained with either 1:20 anti-HLA-A,B,C-PE (clone W6/32, BD Biosciences), 1:100 anti-β2m-FITC (clone 2M2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton-X (proliferating abl pre-B cells) or 0.5% Triton-X (MCF10A cells) in PBS and fixed with BD Cytofix/Cytoperm solution (BD Biosciences, #554722) containing 4.2% formaldehyde ...
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bioRxiv - Immunology 2019Quote: ... IgGpos B cells (100,000 cells from each donor) were sorted on a FACSAria II cell sorter (BD Biosciences, San Jose, CA) and collected for scBCR-seq (Supplementary Fig ...
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bioRxiv - Immunology 2019Quote: ... IgM-negative B cells were purified from lymphocytes using biotinylated anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) and magnetic separation (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Spike-specific memory B cells were stained and single-cell-sorted from PBMCs using a three-laser FACSAria cell sorter (BD Bioscience) by gating on live ...
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bioRxiv - Immunology 2020Quote: ... A maximum of 10,000 live HA-specific mature B cells (CD3-B220+IgD- rHA+) were sorted and collected in a BD FACSAria fusion or BD FACSAria III (BD Biosciences) cell sorter and processed immediately.
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bioRxiv - Immunology 2020Quote: ... naïve splenic B cells were enriched by the depletion of CD43 expressing cell using biotinylated anti-CD43 antibody (Clone S7, BD Biosciences), BD IMag Streptavidin Particles Plus and the IMag® system (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We selected cells by their yellow fluorescence intensity according to the selection criteria described in Figures 2A-B with an Aria III cell sorter (BD Biosciences). Specifically ...
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bioRxiv - Immunology 2022Quote: Fentanyl-reactive B cells were analyzed on a LSR II instrument and isolated using an ARIA II cell sorter (BD Bioscience). Cells were stained using fentanyl-PE (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... B and T-lymphocytes and CD11c+ phagocytes were sorted on a FACSAria IIu Flow Cytometry Sorter (BD Biosciences, San Jose, CA) and into 0.22 µm Spin X centrifuge tube filters (Corning ...
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bioRxiv - Immunology 2020Quote: ... 5×106 cells were stained for 30 min at 4°C with the fluorescent probes, a viability marker (LiveDead-eF780, eBiosciences) and the following B cell-specific antibodies: anti-CD20-PE-CF594 (2H7; BD Biosciences), anti-IgG-PE-Cy7 (G18-145;BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... BCR-expressing B cells were FACS sorted on IgG and GFP double-positivity using a FACS Aria-II SORP (BD biosciences). B cells were then expanded and cultured indefinitely.
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bioRxiv - Immunology 2020Quote: ... The RBD+ frequency of sorted B cells was analyzed post-sort using the index file data in Flow Jo version 9.9.4 (Becton, Dickinson and Company).
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bioRxiv - Immunology 2021Quote: For Reverse Transcription PCR demonstrating 3BNC117 gene integration into the IgH locus, RNA was extracted from sorted engineered B cells (3BNC117+, CD4-, CD19+) on a FACS BD AriaIII (BD Biosciences) using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... PD-L1low and non-B cells from the splenocytes population was carried out by cell sorting in a FACSAria II (BD Biosciences) using different gates strategies (see Supplementary Fig.1).
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bioRxiv - Immunology 2020Quote: ... CD138 (plasma cell marker) and other B cell surface molecules on a flow cytometer (BD LSR II™ or Celesta™). To analyzed immune cells in vivo ...
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bioRxiv - Immunology 2022Quote: ... all cells were stained for L/D staining and acquisition was performed on the Fortessa LSR flow cytometer (BD B ioscience) and analyzed with Kaluza (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... and the antigen-specific B cell population was identified by flow cytometry using a BD ACCURI C6 flow cytometer (BD Biosciences). The non-parametric Kruskal–Wallis test was used to compare the medians (H statistic ...
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bioRxiv - Genomics 2022Quote: ... single GC Light Zone B cells (B220+ GL7+ Fas+ CD86+ CXCR4-) were sorted into in lysis buffer using Aria III flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Single-cell sorting of 7AAD−PfCSP+CD19+ B cells gated positive for either IgG or CD27 as PfCSP memory B cells and 7AAD−CD38+CD27+CD19+ cells as plasmablasts was performed using a FACS Aria II (BD Biosciences) with FACSDiva software (version 8.0.1 ...
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bioRxiv - Immunology 2023Quote: ... FO B (CD19+B220hiCD43− CD23+CD21lo) cells and ABCs (CD19+B220hiCD43−CD23−CD21loCD11b+CD11c+) were purified by FACS Melody (BD Biosciences) as shown in Fig ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... ProB (34+38+19+) and V.) PreB/Mature B-cells (34-19+) were purified by flow cytometry (FACS Aria, BD-Pharmingen). Data acquisition and analysis were done with CellQuest (Beckon Dickinson ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: Mb1-YFP+ B-pDC (B220+ PDCA1+YFP+) and Mb1-YFP- pDCs (B220+PDCA1+YFP-) subsets were sorted on a FACS Aria (BD Biosciences) by and cultured in 96-well round bottom plates (n=3 wells in triplicates ...
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bioRxiv - Immunology 2019Quote: ... Mice were immunized subcutaneously with 50 μg of MOG35-55 peptide emulsified in complete Freund’s adjuvant (CFA, BD Difco, Franklin Lakes, NJ, USA) containing 500 μg of killed Mycobacterium tuberculosis (Strain H37a ...
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bioRxiv - Immunology 2020Quote: ... Isolated LNMCs were incubated with SIV peptide pools covering gag and env in the presence of co-stimulatory αCD28 and αCD49d (BD, 10 μg/mL) on goat-α-mouse IgG F(ab)2-coated (KPL ...
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bioRxiv - Immunology 2020Quote: ... and subsequently PBMCs were stimulated in R10 media with specific peptide pools or medium alone (DMSO control) in the presence of 1 μg/ml of α-CD28 (BD bioscience 555725) and α-CD49d (BD bioscience 555501 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... To detect sheep RBC-induced GC, DZ and LZ B cells, splenocytes were incubated with directly conjugated monoclonal antibodies (Anti-CD19-PE, (BD Bioscience, 7073631), anti-B220-BV510 (BioLegend ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for IFNγ and granzyme B was conducted in Perm/Wash buffer after permeabilization in Fix/Perm buffer (BD Pharmingen, USA) according to kit instructions.
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 1 h with primary antibodies to RAD51 (B-bridge International, #70-001) and to cyclin A (BD Biosciences, # 611268) diluted in 1% BSA ...
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bioRxiv - Immunology 2020Quote: ... and 3-Iodo-L-tyrosine-treated B cells were labeled by using the Annexin V Fitc Apoptosis detection kit (BD, Heidelberg, Germany) according to manufacturer`s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Microbiology 2024Quote: ... Stained memory B cells (MBCs; CD3/CD14/CD56- CD19+ CD27+ IgD- IgM-) were single-cell sorted with a BD FACSAria™ Fusion (BD Biosciences) into 384-well microplates containing 3T3-CD40L feeder cells and were incubated with IL-2 and IL-21 for 14 days71.
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bioRxiv - Neuroscience 2020Quote: EAE was induced in 7-week-old female C57BL/6J mice by subcutaneous flank administration of 200 μg MOG35-55 peptide emulsified with complete Freund’s adjuvant (CFA) (MOG35-55/CFA) (BD Biosciences, San Jose, CA, USA) supplemented with inactive ...
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for IFN-γ and granzyme B was conducted in Perm/Wash buffer after fixation and permeabilization in Fix/Perm buffer (BD Biosciences, USA) according to kit instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... FGF10 expression was induced by the addition of isopropyl-1-thio-B-D-galactopyranoside to RosettaTM2(DE3)pLysS carrying pET21d-FGF10 in 2x YT medium (BD Biosciences, Cat#244020) with Carbencillin (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...