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Citations for Becton, Dickinson and Company :
251 - 300 of 354 citations for Peptide B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Immunology 2024Quote: Splentocyte samples were either aliquoted in mFACS Buffer for staining or resuspended in mR10 and stimulated ex vivo with LCMV peptide DbGP33 (0.2 μg/mL) in the presence of Golgi Plug and Golgi Stop (BD Bioscience) for 5 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: Lymphocytes isolated from the spleen or siIEL compartment of naïve QFLTg_WT or OT-1_WT chimera mice were cultured in vitro with 2µM of FL9 or SL8 peptide respectively together with GolgiPlug (BD Biosciences) for 4.5h ...
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bioRxiv - Microbiology 2020Quote: ... the isolated cells were incubated with mixtures of consecutive 15mer peptides (overlapping by 11AA) of the Mtb Tb6Ag in the presence of the costimulatory molecules CD28 and CD49d (BD Biosciences) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with short peptides for 5h in the presence of brefeldin A or with long peptides for 8h of which the last 6h in presence of brefeldin A (Golgiplug; BD Pharmingen). Flow cytometric acquisition was performed on a BD Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2022Quote: Lymphocytes were isolated from the lungs of VSV-OVA-challenged mice and stimulated ex vivo with OVA peptide 1 mM) in the presence of Golgi-Plug (BD Biosciences) for 5 hours ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Plant Biology 2021Quote: ... The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information) was cloned into the bait vector pGBKT7 (BD-AtCGL160), whereas the coding sequences of CF1-α ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: ... or with stimulation by a total PCS peptide pool (PCS1-12, Table S11) were transferred to 12 × 75mm3 polystyrene tubes (BD Falcon) and washed with FACS wash (PBS supplemented with 2% FBS) ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2020Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 μM Bl-Eng2 or calnexin peptide and 1μg anti-mouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2020Quote: ... stimulated with 1 μg/ml LCMV NP118-126 peptide for 1 hour and then in the presence of GolgiPlug (BD Biosciences) and peptide for an additional 4 hours ...
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bioRxiv - Immunology 2021Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 µM Bl-Eng2 peptide and 1µg antimouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Immunology 2019Quote: ... Half of the enriched B cells were stained with anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) conjugated to PE Cy7 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed and permeabilized for 16 hours according to manufacturer’s instructions for staining with anti-FOXP3 (236A/E7, PE, eBioscience) and anti-Granzyme-B (GB11, BV421, BD Biosciences). Cells were then fixed for subsequent analysis on a Fortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Naive and germinal center B cell purity (>90%) was determined by flow cytometry analysis of surface IgD (BD Biosciences 555778), CD77 (BioRad MCA579 ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Microbiology 2020Quote: ... B cell Ca2+ influx was monitored by measuring the 379/450 nm emission ratio on a LSR Fortessa (BD Biosciences). Following 30 s of baseline measurement ...
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bioRxiv - Microbiology 2021Quote: ... CD21 BV786 (Clone B-LY4, #740969), HLA DR BB700 (Clone G46-6, #566480), CD27 APC R700 (Clone M-T271, #565116) all from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2019Quote: ... and 9g4-naïve B cells (CD27− IgD+ 9g4−) were obtained by FACS sorting on a BD FACSAria II (BD Biosciences). Purified samples were pelleted and stored at −80°C.
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bioRxiv - Genomics 2019Quote: ... Dendritic cells and basophils were sorted as CD3−CD19−CD11c+HLA-DR+ (DCs) and CD3−CD19−CD123+ (basophils) using the following antibodies: CD11C-PECy7 (Clone B-ly6, BD), HLA-DR-APCCy7 (Clone G46-6 ...
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bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We quantified the bias as the proportion of droplets containing pure (A, B, C or D) and compared to mixed populations (AB, AC, AD, BC, BD, CD ...
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bioRxiv - Immunology 2020Quote: ... CD19+CD27+IgG+ memory or CD19+CD27-IgG- naive B cells were isolated using an Aria II sorter (BD Biosciences). The sorted B cells were then added to the irradiated CD40L+ 3T3 cells at a density of 500-25000 cells/well ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... DOT cells were incubated after membrane labelling with anti-perforin-BV241 (dG9) and anti-granzyme B-BV510 (GB11) from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Live Naïve B cells were obtained by sorting the stained cells using a FACS (fluorescence-activated cell sorting) Aria (BD Biosciences) as 7-AAD-CD19+CD38-IgD+CD27- ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg/ml of anti-CD28 (clone 37.51) and 1 μg/ml of anti-CD49d (clone 9C10-MFR4.B) mAbs (BD Biosciences). During the last 3h of incubation ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
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bioRxiv - Immunology 2023Quote: ... transitional (CD19+CD3- B220+CD93+) and mature B cells (CD19+CD3-B220+CD93-) on BD FACSAria Fusion and FACSAria II (BD), before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2 ...
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bioRxiv - Biophysics 2023Quote: The FRET A/D values were mapped onto the thermodynamic model using one fluorescence coefficient (C1) to describe monomeric and low-FRET forms of BRAF in solution (B, BD), and one fluorescence coefficient (C2 ...
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bioRxiv - Bioengineering 2024Quote: ... the B cells were subjected to single-cell sorting using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ, USA) and collected into 96-well plates pre-loaded with lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
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bioRxiv - Microbiology 2020Quote: ... cells were restimulated for 5 hours with the same peptide pool in the presence of brefeldin A (BD Biosciences, San Diego, CA). Cells were then tested for IFN-γ ...
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bioRxiv - Cell Biology 2021Quote: ... C-peptide and Glucagon labelled cells were detected on an LSRII flow cytometer with FACSDiva™ Software (BD Biosciences, San Jose, CA) using the 405- ...
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bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...